Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,203,926 G→T 12.9% L72L (CTG→CTT)  PP_4579 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,203,9260GT12.9% 100.6 / 2.5 31L72L (CTG→CTT) PP_4579LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (14/13);  new base T (2/2);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GCTTCGGTATCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGACCGAGCG  >  NC_002947/5203798‑5204054
                                                                                                                                |                                                                                                                                
gCTTCGGTATCCAGCCGCATTGCCGCACTGGAGGGCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTTCTTGAATACGCCGAACGCATGCTGGAAACCg                                                                                                                           <  1:204935/136‑1 (MQ=255)
gCTTCGGTATCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCg                                                                                                                           <  1:367464/136‑1 (MQ=255)
gCTTCGGTATCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCg                                                                                                                           <  1:364535/136‑1 (MQ=255)
 cTTCGGTATCCAGCCGCATTGCCGCACTGGAGGCAGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGc                                                                                                                          >  2:195441/1‑136 (MQ=255)
  ttCGGTATCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGcc                                                                                                                         <  1:347162/136‑1 (MQ=255)
       tATCCAGCCGCATTGCCGCACTGGAGGCAGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGc                                                                                                                    <  1:195441/136‑1 (MQ=255)
        aTCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGcc                                                                                                                   >  1:165982/1‑136 (MQ=255)
                         cACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGa                                                                                                  <  1:225042/136‑1 (MQ=255)
                             ggAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGc                                                                                              <  2:235952/136‑1 (MQ=255)
                             ggAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGc                                                                                              <  1:227519/136‑1 (MQ=255)
                                            cGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTc                                                                               >  2:102810/1‑136 (MQ=255)
                                            cGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTc                                                                               <  2:249338/136‑1 (MQ=255)
                                             gTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCg                                                                              >  1:211417/1‑136 (MQ=255)
                                                   ctgctgctgCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCa                                                                        >  1:33234/1‑136 (MQ=255)
                                                       tgctgcGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCg                                                                    >  2:371602/1‑136 (MQ=255)
                                                         ctgcGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCa                                                                  >  1:7366/1‑136 (MQ=255)
                                                             gcgACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCgg                                                              <  2:385696/136‑1 (MQ=255)
                                                                                ccTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATc                                           <  1:457709/136‑1 (MQ=255)
                                                                                       ccGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCt                                    >  1:197560/1‑136 (MQ=255)
                                                                                             ggCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGa                              >  1:349276/1‑136 (MQ=255)
                                                                                                   aaGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAgcgcgc                        >  1:167780/1‑136 (MQ=255)
                                                                                                        aCTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCtggtg                   <  2:487561/136‑1 (MQ=255)
                                                                                                                    cGAACGCATGCTTGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAgcgc                          >  1:399805/1‑117 (MQ=255)
                                                                                                                    cGAACGCATGCTTGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAgcgc                          >  1:397584/1‑117 (MQ=255)
                                                                                                                    cGAACGCATGCTTGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAgcgc                          <  2:397584/117‑1 (MQ=255)
                                                                                                                    cGAACGCATGCTTGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAgcgc                          <  2:399805/117‑1 (MQ=255)
                                                                                                                    cGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGAcc       >  1:184559/1‑136 (MQ=255)
                                                                                                                    cGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGAcc       >  2:387652/1‑136 (MQ=255)
                                                                                                                      aaCGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGACCGa     <  2:400085/136‑1 (MQ=255)
                                                                                                                       aCGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGACCGAg    >  1:245088/1‑136 (MQ=255)
                                                                                                                         gCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGACCGAgcg  >  1:191492/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                
GCTTCGGTATCCAGCCGCATTGCCGCACTGGAGGCCGACCTGGGCGTGAAGCTGCTGCTGCGCGACTCCCGCGGCGTCAGCCTGACCCCGGAAGGCAGCAAGGTACTGGAATACGCCGAACGCATGCTGGAAACCGCCAAGGCCATGAAGCAATCGCTGGACAGCGACCGGGCCAAGGTCGGGCGCATTCGCATCGGGGTGATGGATACTGTGATCCACACCTGGATGAGCGCGCTGGTGGCGGAGCTGACCGAGCG  >  NC_002947/5203798‑5204054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑