| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,274,792 | C→A | 23.5% | E182* (GAG→TAG) | radA ← | DNA repair enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,274,792 | 0 | C | A | 23.5% | 36.4 / 3.6 | 17 | E182* (GAG→TAG) | radA | DNA repair enzyme |
| Reads supporting (aligned to +/- strand): ref base C (5/8); new base A (2/2); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01 | |||||||||||
AGCCAGCGAGCCCTCCTTGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTGGGGTAGGCCCAGCCGCCGCGAGC > NC_002947/5274661‑5274926 | agccagcGAGCCCTCCTTGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTg < 1:65995/136‑1 (MQ=255) agcGAGCCCTCCTTGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGaaaa > 1:413859/1‑136 (MQ=255) cctTGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCTGTGAAAATGGTCTGGAt < 2:392919/136‑1 (MQ=255) tGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTAGGTGAAAATGGTCTGGATCGa > 1:128366/1‑136 (MQ=255) tGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTAGGTGAAAATGGTCTGGATCGa > 1:342981/1‑136 (MQ=255) tCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTc < 1:280254/136‑1 (MQ=255) ccGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCa > 1:26707/1‑136 (MQ=255) gcACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGt < 2:413859/136‑1 (MQ=255) cTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCgg < 2:254697/136‑1 (MQ=255) cTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCCCCATTACCCGCGGCTTCACGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTg > 1:164066/1‑136 (MQ=255) cTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTg < 1:366552/136‑1 (MQ=255) cTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTAGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACACGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTg < 2:128366/136‑1 (MQ=255) cTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTAGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACACGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTg < 2:342981/136‑1 (MQ=255) cccGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGCCCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTggg > 2:415881/1‑136 (MQ=255) gggCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTGGGGTAg > 2:45621/1‑136 (MQ=255) gCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTGGGGTAGGCCCAGCCGCCGCGa < 2:96803/136‑1 (MQ=255) tCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTGGGGTAGGCCCAGCCGCCGCGAgc < 1:45621/136‑1 (MQ=255) | AGCCAGCGAGCCCTCCTTGGTCACGTGGCCGACCAGGAAGATTGCCGTACCGCTTTGCTTGGCATAGCGCACCAGCAACGCTGTGCTTTCGCGCACCTGGGCCACGCCGCCCGGGGCCGATTGCAACTGCTCGGTGAAAATGGTCTGGATCGAGTCGATCACCATTACCCGCGGCTTCTCGACCCGCGCCGTGGCAATGATCGTCTCGATGCAGGTTTCGGTCATCACCTTGAGTTGGTCCTGGGGTAGGCCCAGCCGCCGCGAGC > NC_002947/5274661‑5274926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |