| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,455,074 | G→T | 25.0% | D316Y (GAC→TAC) | ybeZ → | nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,455,074 | 0 | G | T | 25.0% | 36.4 / 3.8 | 16 | D316Y (GAC→TAC) | ybeZ | nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base T (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCACCAAGTCGGGCCTGGCGCACGTCATCGAGGTGCTGAAGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGAATTGGCGTTGC > NC_002947/5454948‑5455204 | ggCACCAAGTCGGGCCTGGCGCACGTCATCGAGGTGCTGAAGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCg < 2:445751/136‑1 (MQ=255) cACCAAGTCGGGCCTGGCGCACGTCATCGAGGTGCTGAAGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGaa > 2:278625/1‑136 (MQ=255) cATCGAGGTGCTGAAGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCg > 1:151275/1‑136 (MQ=255) aaGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACTACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATg > 2:49709/1‑136 (MQ=255) aaGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATg > 2:385472/1‑136 (MQ=255) cGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTg > 1:222400/1‑136 (MQ=255) aTCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATc < 1:390409/136‑1 (MQ=255) tttACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACgg > 2:412681/1‑136 (MQ=255) aaCCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACTACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGAtgctg < 1:49709/136‑1 (MQ=255) aaCCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGAtgctg < 1:385472/136‑1 (MQ=255) ttCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGc < 1:472778/60‑1 (MQ=255) ttCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGc > 2:472778/1‑60 (MQ=255) ggTTCAGCGTATCGTCGAAGCCTACTACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGcgccg > 2:278724/1‑136 (MQ=255) ttCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCt < 2:101331/136‑1 (MQ=255) tcgAAGCCTACTACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGAATTgg < 1:278724/136‑1 (MQ=255) ccTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGAATTGGCGTTgc > 2:406155/1‑136 (MQ=255) | GGCACCAAGTCGGGCCTGGCGCACGTCATCGAGGTGCTGAAGGACGTTCCGGGGATCAGCTTTACCCATTTCCAACCCAAGGATGTGGTTCGTCACCCACTGGTTCAGCGTATCGTCGAAGCCTACGACCGCTTCGAAGCCCGCCAGCCCAAGCCCGAGGCGTCCGGCAAAGATGCTTGAACTTGATATCCAACGGGCCACGGATGCTGCCACTCCAGAAGACGCCGCCTTGCGCCGCTGGTGCGAATTGGCGTTGC > NC_002947/5454948‑5455204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |