| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,464,830 | C→A | 16.0% | P84P (CCC→CCA) | holA → | DNA polymerase III subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,464,830 | 0 | C | A | 16.0% | 69.1 / 2.6 | 25 | P84P (CCC→CCA) | holA | DNA polymerase III subunit delta |
| Reads supporting (aligned to +/- strand): ref base C (11/10); new base A (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CCGCGCGTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGACCAAGTGGGGCAAGGCATT > NC_002947/5464697‑5464964 | ccGCGCGTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTc > 1:72265/1‑136 (MQ=255) gTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCaa > 2:295419/1‑136 (MQ=255) gTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCATCGGGCaa > 1:318117/1‑136 (MQ=255) gTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCATCGGGCaa > 1:41073/1‑136 (MQ=255) tATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCcgctgcgct > 2:46152/1‑136 (MQ=255) ccGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATa > 1:384931/1‑136 (MQ=255) aCGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTg < 2:402590/136‑1 (MQ=255) aCGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTg > 2:287148/1‑136 (MQ=255) aCCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGaca < 2:286574/136‑1 (MQ=255) aCCCTGCTCCAGGCCGGTGCAAGCCTGACGCTGTTCGCCCAGCGCCGCCTCCAGGAAATGCGCCTGCCCTCGGGAAAGCCAGGTGACAAGGGTCCCGCTCCGCTGATGGAAAAGTGCGCCACCCCCGCCGAAGacc > 2:335832/1‑135 (MQ=255) gCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACgctg > 1:245975/1‑136 (MQ=255) cAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACgctgctgc > 2:458079/1‑136 (MQ=255) cGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATc < 1:313466/136‑1 (MQ=255) cAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCt > 1:42897/1‑136 (MQ=255) tGTCGCTGTTCGCCCAGCACCACCTGCTGGAACTCCGCCTACCCTCCCGCAACCCCGGTGACAAGAGTGCCGCTACGCTGATGGAATACTACACCAACCCCCCCGAAAACACACAGCAGCTGATCAACCTACCCaa > 2:265699/1‑136 (MQ=255) cTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTcc > 2:266144/1‑135 (MQ=255) cGCCCAGCGCCGCCTGCTGGAACTGCGCCGGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGAcagc < 2:336026/136‑1 (MQ=255) aGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTgc < 2:384931/136‑1 (MQ=255) aGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTgc < 1:287148/136‑1 (MQ=255) ctgctgGAACTGCGCCTGCCATCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCagaaga < 2:318117/136‑1 (MQ=255) ctgctgGAACTGCGCCTGCCATCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCagaaga < 2:41073/136‑1 (MQ=255) gctgGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGAcc < 1:474683/136‑1 (MQ=255) gctgGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGAcc < 1:46152/136‑1 (MQ=255) aCTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGACCAAGTgt < 1:458079/136‑2 (MQ=255) aCTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGACCAAGTgg < 2:355860/136‑1 (MQ=255) ccTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGACCAAGTGGGGCAAGGCAtt > 1:32708/1‑136 (MQ=255) | CCGCGCGTCAGCAAGGTTTCGATGAACGCCAGGTATTCAGTGCCGACGCCAACTTCGACTGGGGCACCCTGCTCCAGGCCGGTGCAAGCCTGTCGCTGTTCGCCCAGCGCCGCCTGCTGGAACTGCGCCTGCCCTCGGGCAAGCCCGGTGACAAGGGTGCCGCTGCGCTGATGGAATACTGCGCCAACCCCGCCGAAGACACGCTGCTGCTGATCAGCCTGCCCAAGCTCGACAGCAGTGCGCAGAAGACCAAGTGGGGCAAGGCATT > NC_002947/5464697‑5464964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |