Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,486,149	G→T	15.3%	S214I (AGC→ATC)	PP_4819 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,486,149	0	G	T	15.3%	75.2 / 2.9	26	S214I (AGC→ATC)	PP_4819	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (8/14);  new base T (2/2);  total (10/16)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

ACCTTTGGAGCTCGACCTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATGACGACGAGCCCGTAGA  >  NC_002947/5486015‑5486257                                                                                                                                       |                                                                                                             accTTTGGAGCTCGACCTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGc                                                                                                             <  2:310507/136‑1 (MQ=255)       ggAGCTCGACCTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCg                                                                                                       <  2:167614/136‑1 (MQ=255)             cGACCTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCTAACGCGATGACCAGCCCGCATTCAGCGAACCGCTgcgc                                                                                                 >  1:503598/1‑136 (MQ=255)                ccTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCATCGAACCGCTGCGCATc                                                                                              >  1:410247/1‑136 (MQ=255)                ccTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCATCGAACCGCTGCGCATc                                                                                              >  1:412575/1‑136 (MQ=255)                            aCCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGaa                                                                                  >  2:3216/1‑136 (MQ=255)                              ccGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAAc                                                                                >  1:76066/1‑136 (MQ=255)                                        cACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCATCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACg                                                                      <  2:412575/136‑1 (MQ=255)                                        cACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCATCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACg                                                                      <  2:410247/136‑1 (MQ=255)                                                        ggCAGCAACCTTGACCTGGACATCGATGGCGAGTCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCcgcg                                                      >  2:352371/1‑136 (MQ=255)                                                         gcagcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCTAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcga                                                     <  2:503598/136‑1 (MQ=255)                                                         gcagcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcga                                                     <  2:401234/136‑1 (MQ=255)                                                         gcagcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcga                                                     <  2:47971/136‑1 (MQ=255)                                                          cagcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcgac                                                    <  2:388848/136‑1 (MQ=255)                                                           agcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcgacg                                                   >  1:439943/1‑136 (MQ=255)                                                           agcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcgacg                                                   >  2:352173/1‑136 (MQ=255)                                                           agcaACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcgacg                                                   >  1:150544/1‑136 (MQ=255)                                                              aaCCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGcgacgacg                                                <  1:183665/136‑1 (MQ=255)                                                                                  tGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCGGGGccgg                            >  2:250743/1‑134 (MQ=255)                                                                                      gAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTTAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACgg                        <  1:219477/136‑1 (MQ=255)                                                                                             ccGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATga                 <  1:3216/136‑1 (MQ=255)                                                                                                tGCACTGTGCGGCCGAACGCGATGACCAGCCGGCATTCAGCGAACCGCTGCGCATCAGCGATGACTAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATgacga              <  1:178708/136‑1 (MQ=255)                                                                                                ggCACGGTGCGGCCGAACGCGATGACCTGCCCGCATTCAGCGACCCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATgacga              <  1:200874/135‑1 (MQ=255)                                                                                                ggCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATgacga              <  1:205210/135‑1 (MQ=255)                                                                                                            ccGAACGCGATGACCAGCCCGCATTCCGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAACCGGGCCTGCGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATGACGACGAGCCCGTaga  <  1:354147/136‑1 (MQ=255)                                                                                                            ccGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATGACGACGAGCCCGTaga  <  1:352371/136‑1 (MQ=255)                                                                                                                                       |                                                                                                             ACCTTTGGAGCTCGACCTGGAGCCTGCACCCGGCGAACGCACCGAGCCTACCCTGGGCAGCAACCTTGACCTGGACATCGATGGCGAGCCCCCCGTGCACGGTGCGGCCGAACGCGATGACCAGCCCGCATTCAGCGAACCGCTGCGCATCAGCGATGACGAAACCCCGGAAACGGGCCTGAGCGCCCGCGACGACGAGCCACTGGCGCTGCGCCTAACGGCGCATGACGACGAGCCCGTAGA  >  NC_002947/5486015‑5486257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑