Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 233,545 C→T 100% G548G (GGC→GGT)  PP_0180 → cytochrome c family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947233,5450CT100.0% 81.0 / NA 30G548G (GGC→GGT) PP_0180cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGCTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTG  >  NC_002947/233420‑233674
                                                                                                                             |                                                                                                                                 
gAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGc                                                                                                                         <  2:38964/136‑1 (MQ=255)
gAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGc                                                                                                                         <  2:177946/136‑1 (MQ=255)
      aTCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGc                                                                                                                   <  1:183385/136‑1 (MQ=255)
        ccTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTa                                                                                                                 <  1:227389/136‑1 (MQ=255)
          tGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCATGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTAta                                                                                                               >  1:159513/1‑135 (MQ=255)
          tGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATc                                                                                                               >  1:270898/1‑136 (MQ=255)
               tACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGt                                                                                                          >  2:181565/1‑136 (MQ=255)
                 cGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGttc                                                                                                        >  1:76449/1‑136 (MQ=255)
                              cTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcg                                                                                           >  1:419857/1‑136 (MQ=255)
                              cTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcg                                                                                           >  1:331614/1‑136 (MQ=255)
                                gcaggcagGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcgcc                                                                                         <  2:495462/136‑1 (MQ=255)
                                gcaggcagGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcgcc                                                                                         <  1:344988/136‑1 (MQ=255)
                                gcaggcagGCCCCGCCGGGCAGCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTCCTTCAGCATCAAcgcc                                                                                         <  1:107198/136‑1 (MQ=255)
                                 caggcaggCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAAcgccg                                                                                        >  1:289947/1‑136 (MQ=255)
                                           ccgccgGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCtgtg                                                                              >  2:382522/1‑136 (MQ=255)
                                            cgccgGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTgc                                                                             >  1:106424/1‑136 (MQ=255)
                                            cgccgGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTgc                                                                             <  1:204923/136‑1 (MQ=255)
                                            cgccgGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTgc                                                                             <  1:147413/136‑1 (MQ=255)
                                                                          cgctACGGCACTGGTGCTGTTGGTTGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGc                                               >  1:111682/1‑136 (MQ=255)
                                                                                       gTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGc                                  >  2:462487/1‑136 (MQ=255)
                                                                                        tGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCa                                 <  1:181565/136‑1 (MQ=255)
                                                                                            gTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAagag                             <  2:270898/136‑1 (MQ=255)
                                                                                             ttGGTGGGCCTGGCCTGGGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAgg                            >  1:375669/1‑136 (MQ=255)
                                                                                                     cctggcctggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGGGCTGTCGGTAGTATTCGCCGGGCATGGCGTGATGGGGTTGCAAGTGGCCGGtgtg                    <  2:106424/136‑1 (MQ=255)
                                                                                                          cctggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTggg               <  1:382522/136‑1 (MQ=255)
                                                                                                           gtggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGc              <  2:329259/135‑1 (MQ=255)
                                                                                                            tggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGca             >  2:224874/1‑136 (MQ=255)
                                                                                                            tggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGca             >  1:67862/1‑136 (MQ=255)
                                                                                                            tggGTGATTCTGCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGATGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGcc             >  2:366711/1‑135 (MQ=255)
                                                                                                                       gCGGGGTTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTg  <  1:111904/136‑1 (MQ=255)
                                                                                                                             |                                                                                                                                 
GAAGTGATCCTGTTCTACGAGACCCTGTGGCTGCAGGCAGGCCCCGCCGGGCATCAGGCGGTGCTGGCGGGTGGCGCGACGGCACTGGTGCTGTTGGTGGGCCTGGCCTGGGTGATTCTGCGGGGCTCGGCCAAGCTGCCGCTATCGCTGTTCTTCAGCATCAACGCCGCGCTGCTGTGCGCGCTGTCGGTAGTATTCGCCGGGCATGGCGTGAAGGCGTTGCAAGAGGCCGGTGTGCTGGGCACACGGCCAGTG  >  NC_002947/233420‑233674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑