| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 354,133 | G→T | 21.0% | S67* (TCG→TAG) | cbcV ← | choline/betaine/carnitine ABC transporter ATP binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 354,133 | 0 | G | T | 21.0% | 46.2 / 3.5 | 19 | S67* (TCG→TAG) | cbcV | choline/betaine/carnitine ABC transporter ATP binding protein |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base T (2/2); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
GCTTGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGCCGGTCTGCTTGAGGATCTGCTCGCGGGTCTGGCCTTGGTCC > NC_002947/354005‑354261 | gcTTGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGcc > 2:114692/1‑136 (MQ=255) ttGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACtt < 2:418884/131‑1 (MQ=255) ttGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACtt > 1:418884/1‑131 (MQ=255) ttGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCg > 1:414822/1‑136 (MQ=255) gcgcATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCg < 2:414822/136‑1 (MQ=255) catcTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACaac > 2:195067/1‑135 (MQ=255) catcTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAg > 2:187841/1‑136 (MQ=255) gCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAg > 1:199102/1‑136 (MQ=255) cgcgGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCTACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAg > 1:180298/1‑136 (MQ=255) cgcgGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCTACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAg > 1:413410/1‑136 (MQ=255) gtGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCtt < 1:78327/135‑1 (MQ=255) tCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCTACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCtttt < 2:180298/136‑1 (MQ=255) tCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCTACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCtttt < 2:413410/136‑1 (MQ=255) gaACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGAcacccacc > 1:226023/1‑136 (MQ=255) aacTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACccaccacca < 2:482756/136‑1 (MQ=255) tGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGcc < 2:226023/136‑1 (MQ=255) cagGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGCCGGTCTGCTTGAGGATCTGCTCGCGGGTCTGGCCTTGGTcc < 1:114692/136‑1 (MQ=255) cagGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGCCGGTCTGCTTGAGGATCTGCTCGCGGGTCTGGCCTTGGTcc < 1:195067/136‑1 (MQ=255) cagGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGCCGGTCTGCTTGAGGATCTGCTCGCGGGTCTGGCCTTGGTcc < 1:187841/136‑1 (MQ=255) | GCTTGGTGCGCATCATCTTCAGCTCGGCCGCGGAGCAGTTGGCAATGTCGATGTGCTTGCCTTCGTGTTCGACGAACAACTTGCCACGGCTGACGGTGTTGAGGCCGTTGATACAGCGCAGCAGGCTCGACTTGCCCGAGCCGGACAAGCCCATCAGCACGCAGATCTCGCCTTTGTTGATATCGAGGTTGGCCTTTTCGACACCCACCACCAGGCCGGTCTGCTTGAGGATCTGCTCGCGGGTCTGGCCTTGGTCC > NC_002947/354005‑354261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |