| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 435,616 | C→A | 13.8% | intergenic (‑414/‑36) | glcB ← / → PP_0357 | malate synthase G/ACT domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 435,616 | 0 | C | A | 13.8% | 92.0 / 2.6 | 29 | intergenic (‑414/‑36) | glcB/PP_0357 | malate synthase G/ACT domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (15/10); new base A (2/2); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
GGGAGTAGGTATGTTCGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTGGTAACTGGCT > NC_002947/435484‑435746 | gggAGTAGGTATGTTCGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTgc > 1:440396/1‑136 (MQ=255) aTGTTCGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGgcgc > 2:253054/1‑136 (MQ=255) tCGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGACCGGCGCGGTc > 1:57581/1‑136 (MQ=255) cGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTcc < 1:190581/136‑1 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCg > 1:253780/1‑136 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCg > 2:242165/1‑136 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTATGCCGCCCGGCGCGGTcc > 2:469491/1‑135 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTATGCCGCCCGGCGCGGTcc > 2:306857/1‑135 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTATGCCGCCCGGCGCGGTcc < 1:306857/135‑1 (MQ=255) gCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTATGCCGCCCGGCGCGGTcc < 1:469491/135‑1 (MQ=255) aaCAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGa < 2:253780/136‑1 (MQ=255) cAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAgg < 2:81678/136‑1 (MQ=255) aaTCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCtt < 2:418892/136‑1 (MQ=255) aGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGt < 1:253054/136‑1 (MQ=255) acGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAAttt > 1:397702/1‑135 (MQ=255) gtgCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACaa > 1:416687/1‑136 (MQ=255) gtgCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACaa > 1:332417/1‑136 (MQ=255) gATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAggccg < 1:12967/136‑1 (MQ=255) gCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAAc < 1:242165/136‑1 (MQ=255) cAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTAGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACg > 1:390676/1‑136 (MQ=255) cGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTAtt > 2:38496/1‑111 (MQ=255) cGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTAtt < 1:38496/111‑1 (MQ=255) cGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTg > 1:193963/1‑136 (MQ=255) cGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTg > 2:480293/1‑136 (MQ=255) gATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTgg < 2:332417/136‑1 (MQ=255) gATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTgg < 2:416687/136‑1 (MQ=255) gATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTgg > 2:430527/1‑136 (MQ=255) ccccGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTGGTAAc > 1:171441/1‑136 (MQ=255) aTTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTGGTAActggct > 2:348753/1‑136 (MQ=255) | GGGAGTAGGTATGTTCGCAAAAAACAGGTGGATTGTTCCAGATAAATCTTGAAACAGTACACGATTGGCTGTGCAGATTGCCCTGCGGCAGGTTGCCGCGCCTTGCCTATACTGACTCGATCCCCCGATTTTCTGCCGCCCGGCGCGGTCCGACACGAGGAAGGGCTTGTGGATCATCTTGTACTGACTGTAATTGCCCCTGACAAGGCCGGCCAGGTCGAACGTATTGCCCAGTGCATCGCCGACCACAGTGGTAACTGGCT > NC_002947/435484‑435746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |