Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	105,230	G→T	13.8%	K8N (AAG→AAT)	cynT →	carbonic anhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	105,230	0	G	T	13.8%	85.6 / 2.7	29	K8N (AAG→AAT)	cynT	carbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base G (13/12);  new base T (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

GTGTCATCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGCCACCGCGCAAACCC  >  NC_002947/105128‑105363                                                                                                       |                                                                                                                                      gtgtCATCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGAt                                                                                                      >  2:468101/1‑136 (MQ=255) gtgtCATCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGAt                                                                                                      >  1:415882/1‑136 (MQ=255) gtgtCATCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGAt                                                                                                      >  1:163723/1‑136 (MQ=255)       tCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGcc                                                                                                <  2:128861/136‑1 (MQ=255)               ccTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGca                                                                                        >  2:322949/1‑136 (MQ=255)                    aTAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAGGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACAtcg                                                                                   >  1:320232/1‑136 (MQ=255)                     tAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACAtcgt                                                                                  >  1:198652/1‑136 (MQ=255)                     tAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACAtcgt                                                                                  >  1:48062/1‑136 (MQ=255)                             aTCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCt                                                                          <  1:170685/136‑1 (MQ=255)                             aTCTATCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCt                                                                          <  1:173010/136‑1 (MQ=255)                                     ccGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGACAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCCCCGATGCTCCCCTCAAGCACATCCTCGACGGCTTCCTgcgc                                                                  >  1:480794/1‑136 (MQ=255)                                           aaaTGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCAc                                                            <  1:358813/136‑1 (MQ=255)                                            aacggATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCAcc                                                           >  1:497947/4‑136 (MQ=255)                                                         acacacCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTccc                                              <  1:251533/136‑1 (MQ=255)                                                             acCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTTAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGAt                                          >  2:423876/1‑136 (MQ=255)                                                                  cAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAATGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCa                                     >  1:28380/1‑136 (MQ=255)                                                                  cAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAATGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCa                                     >  1:25944/1‑136 (MQ=255)                                                                   aaGAGGATGAATCATGCCCGTCAAGGACCCATCCAATGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCaa                                    <  2:25944/136‑1 (MQ=255)                                                                   aaGAGGATGAATCATGCCCGTCAAGGACCCATCCAATGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCaa                                    <  2:28380/136‑1 (MQ=255)                                                                   aaGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCaa                                    <  2:27260/136‑1 (MQ=255)                                                                            aaTCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTTATCAGCAAGAGCTGTTc                           >  1:88360/1‑136 (MQ=255)                                                                            aaTCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTc                           >  2:237036/1‑136 (MQ=255)                                                                                 tGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCaagaa                      <  2:198652/136‑1 (MQ=255)                                                                                 tGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCaagaa                      <  2:48062/136‑1 (MQ=255)                                                                                       tCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGc                <  1:468101/136‑1 (MQ=255)                                                                                       tCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGc                <  2:48164/136‑1 (MQ=255)                                                                                          aGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGCCAc             >  1:447395/1‑136 (MQ=255)                                                                                                     aaGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGCCACCGCGCAAAccc  <  2:128398/136‑1 (MQ=255)                                                                                                     aaGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGCCACCGCGCAAAccc  <  2:471072/136‑1 (MQ=255)                                                                                                       |                                                                                                                                      GTGTCATCTAAAACCCTGCATAGCCTGAATCTTTCACCGGTAAAATGGATTTACCTACACACCGTCAAGAGGATGAATCATGCCCGTCAAGGACCCATCCAAGGTCGTACCGCAGGTCCCCGCGGAAAGCGCCGATGCTGCCCTCAAGCACATCGTCGACGGCTTCCTGCGCTTTCACCATGATGTTTTCCCTGATCAGCAAGAGCTGTTCAAGAAGCTCGCCACCGCGCAAACCC  >  NC_002947/105128‑105363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑