| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 976,913 | C→A | 16.6% | P207P (CCC→CCA) | trmJ → | tRNA (cytosine(32)/uridine(32)‑2'‑O)‑methyltransferase TrmJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 976,913 | 0 | C | A | 16.6% | 68.0 / 3.0 | 24 | P207P (CCC→CCA) | trmJ | tRNA (cytosine(32)/uridine(32)‑2'‑O)‑methyltransferase TrmJ |
| Reads supporting (aligned to +/- strand): ref base C (13/7); new base A (2/2); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
CGGTGAAGCACCGGGCAAAGTCGAGAAGGTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAGGTGGTCCGGGGC > NC_002947/976784‑977024 | cGGTGAAGCACCGGGCAAAGTCGAGAAGGTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCAc > 1:38598/1‑136 (MQ=255) aaaGTCGAGAAGGTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTgc > 2:416023/1‑136 (MQ=255) aaaGTCGAGAAGGTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTgc > 2:21833/1‑136 (MQ=255) ggTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTTCGCCGGCTGTAt > 1:136318/1‑136 (MQ=255) ggTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTAt < 2:433889/136‑1 (MQ=255) ggTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTAt < 2:101303/136‑1 (MQ=255) aGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCAGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGt < 2:47707/136‑1 (MQ=255) ctaCCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCg < 1:226752/134‑1 (MQ=255) aCCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGtt > 1:19658/1‑136 (MQ=255) gATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGaaa < 2:38598/136‑1 (MQ=255) aTGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCAAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAAt > 2:352367/1‑136 (MQ=255) aTGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCAAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAAt > 2:354946/1‑136 (MQ=255) cTACGACCACCTGGAGAAAACACTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGc > 1:253893/1‑136 (MQ=255) tACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCa > 1:245659/1‑136 (MQ=255) accTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCAc < 1:250100/136‑1 (MQ=255) accTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCAAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCAc < 1:354946/136‑1 (MQ=255) accTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCAAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCAc < 1:352367/136‑1 (MQ=255) tGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCgc > 1:336700/1‑135 (MQ=255) tGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCgc > 2:107830/1‑135 (MQ=255) tGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCga > 2:12089/1‑136 (MQ=255) tggGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAggtggt > 1:253150/1‑136 (MQ=255) tggGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAggtggt > 2:422802/1‑136 (MQ=255) cGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAGGTGGTCCGGGGc < 2:19658/136‑1 (MQ=255) cGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAGGTGGTCCGGGGc > 2:425144/1‑136 (MQ=255) | CGGTGAAGCACCGGGCAAAGTCGAGAAGGTTGACGCCGCTGAGCTGGCGACCATGGATGAAATGGAGCTGTTCTACGACCACCTGGAGAAAACCCTGGTGGGCATCGGCTTCCTCGACCCCGAAAAGCCCAAGCACCTGATGCCGCGCCTGCGCCGGCTGTATGGGCGGGTGGCGGTCGAGCGTTCGGAAATGAGCATTTTGCGCGGCATCCTCACCGAGACCCAGAAGGTGGTCCGGGGC > NC_002947/976784‑977024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |