| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,498,121 | A→T | 40.0% | L569Q (CTA→CAA) | PP_1306 ← | Pyocin S‑type killer domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,498,121 | 0 | A | T | 40.0% | 0.5 / 7.5 | 10 | L569Q (CTA→CAA) | PP_1306 | Pyocin S‑type killer domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base T (2/2); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GGTGATGATCAGCCGTTCCTGGTCCAGCAGATCAAGCGCGGGGTAACCCTCCTGCTCGGTGACGACAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAGCGCCAGGCTGTAGACCTGCCGCTCGCTGTCGAACGTTGCTGCGCGTACCGCCACCGGC > NC_002947/1498003‑1498255 | ggTGATGATCAGCCGTTCCTGGTCCAGCAGATCAAGCGCGGGGTAACCCTCCTGCTCGGTGACGACAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTTGGCTGGTGGAGTTTccc > 2:109676/1‑136 (MQ=255)ggTGATGATCAGCCGTTCCTGGTCCAGCAGATCAAGCGCGGGGTAACCCTCCTGCTCGGTGACGACAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTTGGCTGGTGGAGTTTccc > 2:111938/1‑136 (MQ=255) tAACCCTCCTGCTCGGTGACGACAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATcc > 1:314159/1‑136 (MQ=255) tAACCCTCCTGCGCGGTGACGACAGGGTTCTGGCGGCTGCGGGTGTCCACGACGGTACCAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATcc < 1:356935/136‑1 (MQ=255) gcgcGGTGACGACTGGGTTCTGGCGGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGAtt < 1:468378/133‑1 (MQ=255) acgacAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTTGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAgcg < 1:109676/136‑1 (MQ=255) acgacAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTTGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAgcg < 1:111938/136‑1 (MQ=255) cAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAGCGCCAGGCTGTAGACCTGCCGCTCGCTGTCGAACGTtgctgc > 2:398493/1‑136 (MQ=255) gCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAGCGCCAGGCTGTAGACCTGCCGCTCGCTGTCGAACGATGCTGCGCGTACCGCCAc > 2:231908/1‑136 (MQ=255) tAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAGCGCCAGGCTGTAGACCTGCCGCTCGCTGTCGAACGTTGCTGCGCGTACCGCCACCGGc < 2:314159/136‑1 (MQ=255) | GGTGATGATCAGCCGTTCCTGGTCCAGCAGATCAAGCGCGGGGTAACCCTCCTGCTCGGTGACGACAGGGTTCAGGCTGCTGCCGGTGTACACGACGGTACCAGGCGGGACTAGCGGTAGGCTGGTGGAGTTTCCCTCATCGCCACCCGGCGCGCTGGCGGGTGTCCAGGTCAGAATCCGTTGCGGATTATCCAGCGCCAGGCTGTAGACCTGCCGCTCGCTGTCGAACGTTGCTGCGCGTACCGCCACCGGC > NC_002947/1498003‑1498255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |