Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	154,906	C→A	19.0%	L130L (CTG→CTT)	yjbB ←	Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	154,906	0	C	A	19.0%	57.3 / 3.2	21	L130L (CTG→CTT)	yjbB	Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base C (9/8);  new base A (2/2);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

AGCATGATGTCGCCGGTCAGTGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCGGCAC  >  NC_002947/154778‑155033                                                                                                                                 |                                                                                                                                aGCATGATGTCGCCGGTCAGTGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCAAGTTggc                                                                                                                          >  2:39713/1‑136 (MQ=255) aGCATGATGTCGCCGGTCAGTGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCAAGTTggc                                                                                                                          >  2:287378/1‑136 (MQ=255)    atgatgTCGCCGGTCAGTGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACAATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTggccgg                                                                                                                       >  2:193322/1‑136 (MQ=255)                     tGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTgcgc                                                                                                      <  1:445110/136‑1 (MQ=255)                            aaCAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGga                                                                                               >  1:169690/1‑136 (MQ=255)                                                          aTCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCAAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTg                                                                 <  1:287378/136‑1 (MQ=255)                                                          aTCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCAAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTg                                                                 <  1:39713/136‑1 (MQ=255)                                                             ggCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCg                                                              >  1:445709/1‑136 (MQ=255)                                                                     tgcctgcACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAg                                                      <  1:25045/136‑1 (MQ=255)                                                                                  aGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAgg                                         >  1:9204/1‑136 (MQ=255)                                                                                          tGCCAGGATGATCAGGCCAAGCCCTATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCTAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTAcc                                 <  2:58670/136‑1 (MQ=255)                                                                                          tGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTAcc                                 <  2:84155/136‑1 (MQ=255)                                                                                           gCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTAccc                                >  1:388299/1‑136 (MQ=255)                                                                                                 atgatCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCTACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCa                          <  2:281330/136‑1 (MQ=255)                                                                                                                ccGATGCCCACCCGGCCCAGTTGGCCGGCCGGTGCCTGCTGGCGCGAAAGGAAGAAGATCACGCCAAGGACGAGCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCca           <  1:23165/136‑1 (MQ=255)                                                                                                                 cGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCcac          >  1:497733/1‑136 (MQ=255)                                                                                                                 cGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCcac          >  1:429690/1‑136 (MQ=255)                                                                                                                     gCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGATGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCg      <  2:238357/136‑1 (MQ=255)                                                                                                                      cccACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCgg     >  2:300643/1‑136 (MQ=255)                                                                                                                      cccACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCgg     >  2:166165/1‑136 (MQ=255)                                                                                                                         aCCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCGGCAc  <  2:9204/136‑1 (MQ=255)                                                                                                                                 |                                                                                                                                AGCATGATGTCGCCGGTCAGTGAGGCAAACAGCACTTTCACGCCTTGGGCTTGGGTGATCGGCGCCGCTGCCTGCACGATAAGTTCGAGTGCCAGGATGATCAGGCCAAGCCCGATGCCCACCCGGCCCAGTTGGCCGGCCCGTGTCTGCTTGCGCGAAAGGAAGAAGATCACGCCAAGGAAGATCAGCAGTGGCGACAGCCAGGACAGGTCGAAGGTCAGTACCCGGGCCATCAGTGCGGTACCCACATCGGCAC  >  NC_002947/154778‑155033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑