| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,092,669 | C→A | 14.8% | intergenic (+490/+428) | PP_1865 → / ← aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,669 | 0 | C | A | 14.8% | 67.3 / 2.5 | 27 | intergenic (+490/+428) | PP_1865/aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Reads supporting (aligned to +/- strand): ref base C (14/9); new base A (2/2); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGA > NC_002947/2092538‑2092769 | tGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGc > 1:194776/1‑136 (MQ=255)tGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGc < 1:85102/136‑1 (MQ=21) cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGt > 2:501/1‑136 (MQ=255) cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGc < 2:476737/126‑1 (MQ=21) cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGc > 1:476737/1‑126 (MQ=21) cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTAGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATAGAGACTACAGCAGGCCGACTGGAGGCATTGCggagggagaa > 1:82840/1‑134 (MQ=18) cGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCAGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGCTGCCCGTCAAgcg > 2:83679/1‑136 (MQ=255) ctcttgctcttgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAg > 1:278711/1‑136 (MQ=35) tgttttGGGTTTTGGTTCTATGCGGGGGGTTGTTCTGGGGACGCAGATTGCGTCTTGAGGTGTCCGAGCTCTGGGCTTGCGTTGGGTGGTGACGGGCATGGTTGCCCGTTAAGCGCTGGGCCCCAGGATGGGGCCt < 2:59300/133‑1 (MQ=2) tgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt < 1:178630/136‑1 (MQ=32) tgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt < 1:501/136‑1 (MQ=32) tttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCa < 2:194776/136‑1 (MQ=32) tGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAgc > 1:448209/1‑136 (MQ=32) tCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt > 2:278408/1‑120 (MQ=32) tCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt < 1:278408/120‑1 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATTGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg > 1:374551/1‑136 (MQ=17) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGcc < 1:254647/117‑1 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGcc > 2:254647/1‑117 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt < 2:403976/118‑1 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCt > 1:403976/1‑118 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg > 1:353761/1‑136 (MQ=32) tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg > 1:453919/1‑136 (MQ=32) aGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCt < 1:449711/97‑1 (MQ=33) aGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCt > 2:449711/1‑97 (MQ=33) aGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTg > 1:117333/1‑117 (MQ=32) aGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTg < 2:117333/117‑1 (MQ=32) aCGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGCGACGGGCATGGATGCCCGTCAAGCGCAGGGCCCCAGGATGGGGCCAGCAGCCCCGTCCTCCCGGGCCCAACCCCGGAGTGCggggc > 1:236517/1‑135 (MQ=11) | TGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGA > NC_002947/2092538‑2092769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |