| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,106,743 | A→C | 46.4% | L186R (CTG→CGG) | PP_1878 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,106,743 | 0 | A | C | 46.4% | ‑6.4 / 3.2 | 13 | L186R (CTG→CGG) | PP_1878 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (2/4); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG > NC_002947/2106617‑2106871 | ggggCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGccc > 1:221046/1‑135 (MQ=255) tggCGGCCCAGTACGTGGGATTGGGCCGGGGCCAACAGTTTCTCAAGAAACGTCAGCTGCTCTACATACCCCTTCGGCGCCGGCGGCTGGCGAAAATACTCCACGCCGCGCTCGACGGCCCGGCGCgccgggccgg < 2:164988/134‑1 (MQ=255) tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGGCCGGCggggcggtt > 2:233763/1‑130 (MQ=255) cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGCCGTTTTTCAgggg > 1:453898/1‑135 (MQ=255) cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCCGGGTGGGCGTTTTTAAgggg > 1:106737/1‑135 (MQ=255) cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCCCCTGGGCGCGCTGGGGGTTTTGCTGGGc > 1:34842/1‑136 (MQ=255) cccAACATTTTCTCATGAGACGTCTGCTGGTCTTCAAACCGCTTCGGCTCGGGCTGGTGGCTCAAATCCGGCCCGCCGCGCTCGTCGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGa < 2:427752/135‑1 (MQ=255) ttttCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGgttgtt < 1:161682/135‑1 (MQ=255) caaaGATCCGTCAGCTGCTCTTCATCCACCTGCGGCGCGGGCGGCGTGCTCAAATACGCCCCGCCGCGCTCGCAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga < 1:427819/134‑1 (MQ=255) gctCTACATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag < 2:336477/136‑1 (MQ=255) gccgcGCTCGAAGGCCAGGCGCGCCTGTCCGGGCTGGCCTTTTTTCAGGGCCTGTTGGCCGAGGGTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTTGCCTCGAcc > 1:254234/1‑135 (MQ=255) tCGAAGGCCAGGCGCGCCTTGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCa > 2:386647/1‑136 (MQ=255) taaGGCCCGGCGCGGCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag < 2:453898/135‑1 (MQ=255) | GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG > NC_002947/2106617‑2106871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |