| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,729,230 | G→T | 16.0% | T118T (ACC→ACA) | azlC ← | branched‑chain amino acid transport protein AzlC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,729,230 | 0 | G | T | 16.0% | 77.8 / 2.9 | 25 | T118T (ACC→ACA) | azlC | branched‑chain amino acid transport protein AzlC |
| Reads supporting (aligned to +/- strand): ref base G (15/6); new base T (2/2); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TGGCCCAGCAGTATGCCGGCCAGGGTGAACAGGTTCCAGGCGATGTAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGTCAGCA > NC_002947/2729123‑2729348 | tGGCCCAGCAGTATGCCGGCCAGGGTGAACAGGTTCCAGGCGATGTAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTcagca > 2:309055/1‑136 (MQ=255) cAGGCGATGTAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCggctggc > 2:334620/1‑136 (MQ=255) tAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATa > 1:383263/1‑136 (MQ=255) tAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATa < 2:361355/136‑1 (MQ=255) aGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATAc > 1:450447/1‑136 (MQ=255) aGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCa > 1:274595/1‑136 (MQ=255) gCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATc > 1:111073/1‑136 (MQ=255) gCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATc > 1:271232/1‑136 (MQ=255) gCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATc > 2:399884/1‑136 (MQ=255) gCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATc > 1:472770/1‑136 (MQ=255) cccACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCg > 1:251415/1‑136 (MQ=255) cACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGAc < 1:210020/136‑1 (MQ=255) cACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGAc < 2:383263/136‑1 (MQ=255) ccAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTTGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGcc > 1:459688/1‑136 (MQ=255) ccAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTTGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGcc > 1:301067/1‑136 (MQ=255) ccAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATtcgtcg > 1:472174/1‑65 (MQ=255) ccAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATtcgtcg < 2:472174/65‑1 (MQ=255) ggCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCAttc < 2:271232/136‑3 (MQ=255) ggCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCAtta < 2:111073/136‑3 (MQ=255) cAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGt > 1:406588/1‑136 (MQ=255) cAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGt > 1:295691/1‑136 (MQ=255) aTTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGt > 2:346928/1‑136 (MQ=255) aTTGCTGCCGGTCGTACTGGCTGGTCAGGGCCAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGt > 2:31342/1‑136 (MQ=255) tgcCGGTCGTACTGGCTTGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGTcagca < 2:301067/136‑1 (MQ=255) tgcCGGTCGTACTGGCTTGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGTcagca < 2:459688/136‑1 (MQ=255) | TGGCCCAGCAGTATGCCGGCCAGGGTGAACAGGTTCCAGGCGATGTAGAAGGTCAGGCCCACCCCCAAGGCATACCAGCGGTTGAATTGCTGCCGGTCGTACTGGCTGGTCAGGGCGAAGAATTCGTCGGTCAGCAGAAAGCCCAGGCCCAGGCGCCAGCGCAGCGGCTGGCTGGACAATACCGGGCGCATCGACAGGCCATACAGCAGGTGCTGCGAGGTCAGCA > NC_002947/2729123‑2729348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |