| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,592,118 | G→A | 30.8% | A440A (GCG→GCA) | benK → | benzoate MFS transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,592,118 | 0 | G | A | 30.8% | 13.5 / 5.8 | 13 | A440A (GCG→GCA) | benK | benzoate MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (3/6); new base A (2/2); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
GCTGATGGGCATCAACCTGCCACTGCAACTCAATTTCATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGTTCTTCGAGCAGGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAAC > NC_002947/3591992‑3592250 | gcTGATGGGCATCAACCTGCCACTGCAACTCAATTTCATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCACAGGCCTGa > 2:368472/1‑136 (MQ=255)gcTGATGGGCATCAACCTGCCACTGCAACTCAATTTCATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCACAGGCCTGa > 2:475665/1‑136 (MQ=255) tGCCACTGCAACTCAATTTCATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGCCCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGAc > 1:455223/1‑136 (MQ=255) cATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCg > 2:91993/1‑136 (MQ=255) tCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTg < 1:476081/136‑1 (MQ=255) cttttGCCATCCGTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAAc < 1:66494/134‑1 (MQ=255) cgcCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCACAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCg < 1:368472/136‑1 (MQ=255) cgcCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCACAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCg < 1:475665/136‑1 (MQ=255) ccATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGttcttc < 2:15228/136‑1 (MQ=255) ccATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGttcttc < 2:19960/136‑1 (MQ=255) cGTGCACCTGGCTCTTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGTTCTTCGAGCAgg < 1:405117/136‑1 (MQ=255) cccACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGTTCTTCGAGCAGGCCGCAGGCTTTTGTAATGcggcc < 1:91993/135‑1 (MQ=255) cccTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGTTCTTCGAGCAGGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGc > 2:371027/1‑136 (MQ=255) ggcgCTGGCCTGAACCGAAGAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGTGGTACAGCAGTTCTTCGAGCAGGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAAc < 1:182262/136‑1 (MQ=255) | GCTGATGGGCATCAACCTGCCACTGCAACTCAATTTCATCGCCTTTGCCATCCCTGGCGCCATCGCTGCGCTGGCCATGGCCGTGCACCTGGCCAGTGGTCGGCGCCACGCCCAGACCCTGGCGGCGCAGGCCTGAACCGAACAGAACAAGACGAGGTAACCCCCATGACCGTGAACATTTCCCATACTGCCGAGGTACAGCAGTTCTTCGAGCAGGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAAC > NC_002947/3591992‑3592250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |