Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,675,665	C→A	28.5%	D154Y (GAC→TAC)	PP_5591 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,675,665	0	C	A	28.5%	25.9 / 3.4	14	D154Y (GAC→TAC)	PP_5591	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base A (2/2);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGGGTGCTACGCCTGTTTGCCAGCACAGCTGGGCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGCAGCCAAC  >  NC_002947/3675530‑3675763                                                                                                                                        |                                                                                                   gcTGGGGTGCTACGCCTGTTTGCCAGCACAGCTGGGCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTc                                                                                                    <  1:34899/136‑1 (MQ=255)                                cTGGGCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACaa                                                                     >  2:455459/1‑136 (MQ=255)                                   ggCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCg                                                                  >  2:348390/1‑136 (MQ=255)                                   ggCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCg                                                                  >  2:407014/1‑136 (MQ=255)                                        ccTGGCGCGCCACCTGTGACGGTGCGCAGAAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTgg                                                             <  1:25871/136‑1 (MQ=255)                                             cgcgcCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGgcagca                                                        >  2:413144/1‑136 (MQ=255)                                                gcCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTAACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGtt                                                     >  2:296200/1‑136 (MQ=255)                                                gcCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTAACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGtt                                                     >  2:452357/1‑136 (MQ=255)                                                                                            ggTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGc         >  2:444747/1‑136 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGAc                              <  1:423126/108‑1 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGAc                              >  2:423126/1‑108 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGCAGCCAAc  >  1:285264/1‑136 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGCAGCCAAc  <  1:348390/136‑1 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTAACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGCAGCCAAc  <  1:296200/136‑1 (MQ=255)                                                                                                   aGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTAACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGAAGCCAAc  <  1:452357/136‑1 (MQ=255)                                                                                                                                        |                                                                                                   GCTGGGGTGCTACGCCTGTTTGCCAGCACAGCTGGGCTACCTGGCGCGCCACCTGTGACGGTGCGCAGCAGTACACCGTGTCCGGGTGAAAGGTGATCAGCAAGCGCCTGGTTTGCCACCAGCCACTGCTGCTGTCACGCGGCAGTAGCGACAGCCAGGTTCGACAATCGTCTGGCAGCAGTTCAACTGCAATGCCGTCATGTGACAGCTGCAATTGTATCCAGGGCAGCCAAC  >  NC_002947/3675530‑3675763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑