| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,783,039 | C→T | 22.1% | S367S (AGC→AGT) | PP_3340 → | TonB‑dependent receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,783,039 | 0 | C | T | 22.1% | 33.6 / 3.7 | 18 | S367S (AGC→AGT) | PP_3340 | TonB‑dependent receptor |
| Reads supporting (aligned to +/- strand): ref base C (6/8); new base T (2/2); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TTCACAGCAGGAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTCGAATCGCTGAAGATCGTG > NC_002947/3782904‑3783162 | ttCACAGCAGGAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGc < 1:307489/136‑1 (MQ=255)ttCACAGCAGGAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGc < 2:439563/136‑1 (MQ=255) gAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGTGAACGCTAcc > 1:183969/1‑136 (MQ=255) gAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGTGAACGCTAcc > 1:186257/1‑136 (MQ=255) aCGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGgc > 2:483442/1‑136 (MQ=255) cATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGacc > 1:417755/1‑135 (MQ=255) aCCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACAcgcg < 1:233553/136‑1 (MQ=255) ccTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACAcgcgc > 2:49056/1‑135 (MQ=255) tGGACTGGCTGCTCGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACAcggt < 1:278766/136‑1 (MQ=255) tGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGTGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACAcggt < 2:183969/136‑1 (MQ=255) tGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGTGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACAcggt < 2:186257/136‑1 (MQ=255) gACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTAc > 1:17318/1‑91 (MQ=255) gACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTAc < 2:17318/91‑1 (MQ=255) gCACGCGTTTGCGCTGGATGGGGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGACCGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTAt < 1:62349/136‑1 (MQ=255) gCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTAt < 1:381665/136‑1 (MQ=255) gCACGCCTTTGCGCTGGAAGGGGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTAt < 1:33589/136‑1 (MQ=255) cAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTc > 2:292566/1‑136 (MQ=255) cAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTc < 1:483442/136‑1 (MQ=255) cAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTCGAATCGCTGa < 2:279575/136‑1 (MQ=255) aCAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTCGAATCGCTGAAGATCGTg > 2:51232/1‑136 (MQ=255) | TTCACAGCAGGAACGCGACACCTACGAGGATCAGTACGGTGCCATCACCTCGCTGACCTGGCGACCACAGGTGGACTGGCTGCACGCCTTTGCGCTGGAAGGTGGCAGCGATATGCAGAAGCAACAGAACCGCAGCGAACGCTACCGCACCGTGGAGCGTGTACGCCAGGCGCAGACACGCGACCAGGACTTCGACTTCAACACGGTCGGTGCCTATGTACAGGCGGAGATCGAGCCGTTCGAATCGCTGAAGATCGTG > NC_002947/3782904‑3783162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |