Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,916,115 C→T 11.7% D144D (GAC→GAT)  PP_3451 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,916,1150CT11.7% 96.3 / 2.5 34D144D (GAC→GAT) PP_3451hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (13/17);  new base T (2/2);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGACGAGCTGGATGGCGAGGGCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGACGCCGCGGGTC  >  NC_002947/3915980‑3916241
                                                                                                                                       |                                                                                                                              
ccTCGACGAGCTGGATGGCGAGGGCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGgac                                                                                                                                >  2:320707/1‑136 (MQ=255)
ccTCGACGAGCTGGATGGCGAGGGCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTAGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGgac                                                                                                                                >  2:40527/1‑136 (MQ=255)
                     gggCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGAcc                                                                                                           <  1:169154/136‑1 (MQ=255)
                     gggCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGAcc                                                                                                           <  1:171398/136‑1 (MQ=255)
                     gggCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGAcc                                                                                                           <  1:424452/136‑1 (MQ=255)
                       gCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGa                                                                                                         <  2:6129/136‑1 (MQ=255)
                       gCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGa                                                                                                         >  2:429399/1‑136 (MQ=255)
                          tGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     <  1:497656/121‑1 (MQ=255)
                          tGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     >  2:497656/1‑121 (MQ=255)
                          tGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     <  2:17379/121‑1 (MQ=255)
                          tGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     >  1:17379/1‑121 (MQ=255)
                               aGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGcgcg                                                                                                 <  2:244918/136‑1 (MQ=255)
                                 cTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGAt                                                                                               <  1:16302/136‑1 (MQ=255)
                                 cTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGAt                                                                                               <  1:279308/136‑1 (MQ=255)
                                    cTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATgcg                                                                                            >  1:476146/1‑136 (MQ=255)
                                    cTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATgcg                                                                                            >  1:83850/1‑136 (MQ=255)
                                     tGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATgcgc                                                                                           <  2:123427/136‑1 (MQ=255)
                                         aaGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTa                                                                                       >  2:366268/1‑136 (MQ=255)
                                                         ctcgctcAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGcc                                                                       >  2:164491/1‑136 (MQ=255)
                                                            gctcAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGATGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTc                                                                    >  1:200473/1‑136 (MQ=255)
                                                                            cAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACa                                                    <  1:429399/136‑1 (MQ=255)
                                                                               cTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCCTCTGCGCGCGACCGACCGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGGTGGACAAGc                                                 >  1:250386/1‑136 (MQ=255)
                                                                               cTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGc                                                 >  1:383113/1‑136 (MQ=255)
                                                                                   gcCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGtt                                             >  2:358238/1‑136 (MQ=255)
                                                                                   gcCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGtt                                             >  2:392456/1‑136 (MQ=255)
                                                                                     cAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     <  2:249280/62‑1 (MQ=255)
                                                                                     cAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCt                                                                                                                     >  1:249280/1‑62 (MQ=255)
                                                                                                    ccAGTGGACGGCTGGTACCTGCCCATGGAGCTGGATGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCa                            <  2:200473/136‑1 (MQ=255)
                                                                                                        tGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGcac                        <  1:320707/136‑1 (MQ=255)
                                                                                                              gCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCaa                  >  1:128466/1‑136 (MQ=255)
                                                                                                                     ccTGCGCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGAcg           <  1:40527/136‑1 (MQ=255)
                                                                                                                     ccTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGAcg           <  1:40088/136‑1 (MQ=255)
                                                                                                                     ccTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGAcg           <  1:338523/136‑1 (MQ=255)
                                                                                                                            aTGGAGCTGGATGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTAc                                                                                      <  1:446902/54‑1 (MQ=255)
                                                                                                                            aTGGAGCTGGATGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTAc                                                                                      >  2:446902/1‑54 (MQ=255)
                                                                                                                              ggAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGACGCCGCGGGTc  <  2:51289/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                              
CCTCGACGAGCTGGATGGCGAGGGCCTGAACAGCTACTGGAAAGCGCAGCTGGGGCGCTCGCTCAGCCAGTACCAGCAACTGCGCCAGGCCTGGCAGTTGCCAGTGGACGGCTGGTACCTGCCCATGGAGCTGGACGACCAGCATCTGCGCGAGACCGAACGGCGCGATGCGCTGTACAGCCAGTTGCAGGCCTTCAACCGCCAGTTGGACAAGCCGTTGCATATCAGCGCCTTCAGCACAGGCAAGCTGACGCCGCGGGTC  >  NC_002947/3915980‑3916241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑