| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,061,752 | G→T | 17.4% | F301L (TTC→TTA) | PP_3575 ← | ferric siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,061,752 | 0 | G | T | 17.4% | 56.7 / 3.1 | 23 | F301L (TTC→TTA) | PP_3575 | ferric siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base G (11/8); new base T (2/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01 | |||||||||||
AGAGACGCAGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAGCGGCTCGCTTGGGCTGCACGTTGAT > NC_002947/4061637‑4061877 | agagaCGCAGGCGCTCGCCTCGAAAATCCAGGCCCATCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCaaa > 2:90011/1‑136 (MQ=255)agagaCGCAGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCaaa > 2:271644/1‑136 (MQ=255)agagaCGCAGGCGCTCGCCTCGAAAATCCAGGCACAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCaaa > 1:392571/1‑136 (MQ=255) gCAGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACg < 2:343735/136‑1 (MQ=255) gCAGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACg > 2:297395/1‑136 (MQ=255) aGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGc > 1:224770/1‑136 (MQ=255) cTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATg > 1:229874/1‑136 (MQ=255) aGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCTAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAg > 1:118597/1‑136 (MQ=255) aGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCTAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAg > 1:445359/1‑136 (MQ=255) aGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAg > 1:296685/1‑136 (MQ=255) gCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGcc > 2:242938/1‑136 (MQ=255) cAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTgg > 1:326402/1‑136 (MQ=255) tttCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCAGAGGc > 2:402828/1‑136 (MQ=255) ggtCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGt < 2:229874/136‑1 (MQ=255) tCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCTAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAg < 2:118597/136‑1 (MQ=255) tCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCTAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAg < 2:445359/136‑1 (MQ=255) tCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAg < 2:296685/136‑1 (MQ=255) tCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAg < 2:446239/136‑1 (MQ=255) tCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCAGAGGCGTAGCTGCCGGTGAATTAGGTGATCGGCTTGTCAg < 1:402828/136‑1 (MQ=255) ttGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAGCGGCTCGCTTGGGCTGCACGt < 2:224770/136‑1 (MQ=255) aGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTTGGTGATCGGCTTGTCAGCGGCTCGCTTGGGCTGCACGTTGAt < 2:392571/136‑1 (MQ=255) cGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGt < 2:230174/88‑1 (MQ=255) cGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGt > 1:230174/1‑88 (MQ=255) | AGAGACGCAGGCGCTCGCCTCGAAAATCCAGGCCCAGCACCGCCATTTCGCGGTCGACGCGCTGGTGGTCCCATTCGGTATCACCGGCCTGCTTCACGCCATTGAAGCGCACGCCGAACTGCTGGCCTTCACCAAAGCGACGGCCGATGTCCACTGCGCCGCCAGCCTGGCCGGCCGAGGCGTAGCTGCCGGTGAATTCGGTGATCGGCTTGTCAGCGGCTCGCTTGGGCTGCACGTTGAT > NC_002947/4061637‑4061877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |