| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,254,683 | G→T | 14.8% | Q113K (CAG→AAG) | PP_3724 ← | acyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,254,683 | 0 | G | T | 14.8% | 84.6 / 2.7 | 27 | Q113K (CAG→AAG) | PP_3724 | acyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (11/12); new base T (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
ACCCCCTTCTCCACTTGCTGGCCACCATGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGTGGCGATGAACACCGCGT > NC_002947/4254553‑4254814 | aCCCCCTTCTCCACTTGCTGGCCACCATGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACt > 2:376461/1‑136 (MQ=255) ggCCACCATGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCt > 1:379687/1‑136 (MQ=255) ggCCACCATGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCt > 1:467635/1‑136 (MQ=255) accaTGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCg < 1:119105/136‑1 (MQ=255) aTGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCa > 2:103747/1‑136 (MQ=255) cGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAg < 2:466574/136‑1 (MQ=255) ccACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCa < 1:337316/136‑1 (MQ=255) cACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGgc > 1:7984/1‑136 (MQ=255) cGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGATGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGcc > 2:470983/1‑136 (MQ=255) gTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACt > 2:47539/1‑74 (MQ=255) gTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACt < 1:47539/74‑1 (MQ=255) gTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGcacca < 2:497054/136‑1 (MQ=255) gAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGt < 1:263506/136‑1 (MQ=255) gCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTTCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGc < 1:273339/136‑1 (MQ=255) gCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTTCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGc < 1:270614/136‑1 (MQ=255) gCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTTCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGc > 2:273339/1‑136 (MQ=255) gCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTTCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGc > 2:270614/1‑136 (MQ=255) gTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCa < 1:338509/136‑1 (MQ=255) ttGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCaa < 2:7984/136‑1 (MQ=255) gCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACCCAc > 1:400934/1‑136 (MQ=255) aaGGCCATGTTGCCTGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAggg > 1:308045/1‑136 (MQ=255) tGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAg > 1:490851/1‑124 (MQ=255) tGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAg < 2:490851/124‑1 (MQ=255) tGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACg > 1:497648/1‑136 (MQ=255) gTCGAGCAGGCGTTGCTGCAACTGGCAGCCATCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGTGGCg < 1:376461/136‑1 (MQ=255) gTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGTGGCGATGAACACcgc < 2:129895/136‑1 (MQ=255) ttGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGGGGCGATGAACACcgcg < 1:470983/136‑1 (MQ=255) tgctgcAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGTGGCGATGAACACCGCGt < 2:497648/136‑1 (MQ=255) | ACCCCCTTCTCCACTTGCTGGCCACCATGCGCCACCGGCCACAGGGCGGCACCGTGCAGGTAGTCGAGCAAGCCATAGCAGTTGGGGCCGAGCAAGGCCATGTTGCCGGCGACGTCGAGCAGGCGTTGCTGCAACTGGCAGCCCTCCTCACCGCTTTCGGCAAAGCCCGAGGCATAGCAGATGGCGCCACCGGCACCACGTGCAGCCAGCTCGGCAACGCACTGCAGGGTCAGCTCACGGTTGGTGGCGATGAACACCGCGT > NC_002947/4254553‑4254814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |