| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,300,295 | C→T | 19.4% | C24Y (TGT→TAT) | opdN ← | outer‑membrane porin D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,300,295 | 0 | C | T | 19.4% | 43.2 / 4.9 | 22 | C24Y (TGT→TAT) | opdN | outer‑membrane porin D |
| Reads supporting (aligned to +/- strand): ref base C (11/6); new base T (2/2); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTTCGCGCTTGGATGCGCCCTGGCTGGAACTGCTGTAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACGTGTTGGCCACGGTACTGCGGCAGG > NC_002947/4300161‑4300423 | ctTCGCGCTTGGATGCGCCCTGGCTGGAACTGCTGTAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGcaca > 2:408510/1‑136 (MQ=255) aTGCGCCCTGGCTGGAACTGCTGTAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAg > 1:221322/1‑136 (MQ=255) ctggctggAACTGCTGTAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTa < 2:467661/136‑1 (MQ=255) ttAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCgggg < 1:408510/135‑1 (MQ=255) tAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCggtgg > 1:169434/1‑136 (MQ=255) tAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCggggg > 1:421997/1‑136 (MQ=255) ggAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGggggaa > 2:128668/1‑136 (MQ=255) ggAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGggggaa > 2:312863/1‑136 (MQ=255) ggAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGggggaa > 1:452727/1‑136 (MQ=255) ggAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAAAAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGggggaa > 2:293953/1‑136 (MQ=255) gAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGT‑ACACGGCCGCCGGGGGGCGAGggggaaa > 2:286661/1‑136 (MQ=255) aTTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAAc < 2:421997/136‑1 (MQ=255) gATCTTGGCACTGGCTTCTTCAAGAAAAAAGGAAGCCTGTGTGTTTAGTCAGGCACACATTAGAAAGAGGCCTTTAACACGT‑CGCCGGGGTGCGATGTGGAAAGGGGAAAACATGCGAGGTAACATCCAGAATGaa < 2:999/136‑1 (MQ=255) cGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTaacaac > 1:421345/1‑136 (MQ=255) cGTCTTCAAGAAACCAGGCAGCCTCGGCGTTGACAACAGCAAACAATAGAAACAGGCCGGTAACAATG‑CTCCCGGGGGCGAGGGCGAAAGCGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTAaacaac > 1:312515/1‑136 (MQ=255) tCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAgggg < 1:479092/80‑1 (MQ=255) tCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAgggg > 2:479092/1‑80 (MQ=255) aaGAAACCCGGCAGCCTGGGCGTTGACACCGGCATACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGc > 2:130180/1‑136 (MQ=255) aaGAAACCCGGCAGCCTGGGCGTTGACACCGGCATACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGc > 2:393589/1‑136 (MQ=255) aaCCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACgt < 1:389073/136‑1 (MQ=255) ggCAGCCTGGGCGTTGACACCGGCATACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACGTGTTgg < 1:130180/136‑1 (MQ=255) ggCAGCCTGGGCGTTGACACCGGCATACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACGTGTTgg < 1:393589/136‑1 (MQ=255) acCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACGTGTTGGCCACGGTACTGCGGCAgg > 2:489095/1‑136 (MQ=255) | CTTCGCGCTTGGATGCGCCCTGGCTGGAACTGCTGTAACCGTCCCGGAAGTCGCGGTTGAAATAGACATTGCGTGCTTCGATCTTGGCACTGGCGTCTTCAAGAAACCCGGCAGCCTGGGCGTTGACACCGGCACACAATAGAAACAGGCCGGTAACACGG‑CGCCCGGGGGCGAGGGGGAAAGGGGAAAACATGCGAGGAAACATCCAGAATGAAGACGAGTTAACAACCTGTGGCACGTGTTGGCCACGGTACTGCGGCAGG > NC_002947/4300161‑4300423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |