| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,641,787 | G→T | 25.0% | S122* (TCG→TAG) | PP_4102 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,641,787 | 0 | G | T | 25.0% | 33.0 / 3.8 | 16 | S122* (TCG→TAG) | PP_4102 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (4/8); new base T (2/2); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
CAGGTCGAATTCACGGTAGTAGGGCAACATGGCGCGGCGCGTAAGGTCGCGGGCGAAGGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTCCCCTTCCTGGCCAGGTCCAGCA > NC_002947/4641656‑4641913 | cagGTCGAATTCACGGTAGTAGGGCAACATGGCGCGGCGCGTAAGGTCGCGGGCGAAGGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACt < 1:21756/136‑1 (MQ=255) cGAATTCACGGTAGTAGGGCAACATGGCGCGGCGCGTAAGGTCGCGGGCGAATGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAg < 1:427383/136‑1 (MQ=255) tGGCGCGGCGCGTAAGGTCGCGGGCGAAGGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGtttt > 1:211458/1‑136 (MQ=255) cgcgGGCGAAGGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCa < 1:113592/136‑1 (MQ=255) gggATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCATGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGc < 1:109043/135‑1 (MQ=255) aTGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAggg > 1:321356/1‑76 (MQ=255) aTGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAggg < 2:321356/76‑1 (MQ=255) ttGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTc > 1:82899/1‑136 (MQ=255) tcgcatcgcaCTCATTGGGCATAACAAACTGGCTAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCg > 2:16068/1‑136 (MQ=255) tcgcatcgcaCTCATTGGGCATAACAAACTGGCTAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCg > 2:434391/1‑136 (MQ=255) tcgcaCTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTcccc < 2:470944/136‑1 (MQ=255) ttGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTCCCCTTCCTGGcc < 2:81992/136‑1 (MQ=255) cAAACTGGCTAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTCCCCTTCCTGGCCATGTCcagca < 1:16068/136‑1 (MQ=255) cAAACTGGCTAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTCCCCTTCCTGGCCATGTCcagca < 1:434391/136‑1 (MQ=255) aaCTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATaagca < 1:301902/100‑4 (MQ=255) aaCTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATaagca > 2:301902/1‑97 (MQ=255) | CAGGTCGAATTCACGGTAGTAGGGCAACATGGCGCGGCGCGTAAGGTCGCGGGCGAAGGTGCAGTGGATGTCTTGGGCGGGAATCAACTGTAAGAACATCGCATCGCACTCATTGGGCATAACAAACTGGCGAACTGCCAGGGCCACTCCTGTCCAGTTCGTTTTCCAACCAGTCGGCCAGCACCCGGGCATTGTTGTGCTCGCTGTCCTTGCCGGCATAAAGCAATGTCAGCGTCCCCTTCCTGGCCAGGTCCAGCA > NC_002947/4641656‑4641913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |