| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,719,906 | G→T | 36.5% | G48W (GGG→TGG) | PP_4173 → | two‑component system sensor histidine kinase/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,719,906 | 0 | G | T | 36.5% | 9.4 / 4.5 | 11 | G48W (GGG→TGG) | PP_4173 | two‑component system sensor histidine kinase/response regulator |
| Reads supporting (aligned to +/- strand): ref base G (3/4); new base T (2/2); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
GCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGCACGT > NC_002947/4719775‑4720015 | gCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCa < 1:400204/136‑1 (MQ=255)gCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCa > 2:363890/1‑136 (MQ=255) gCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCTGGCAACTGAt > 2:118034/1‑136 (MQ=255) gCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCTGGCAACTGAt > 2:478919/1‑136 (MQ=255) caGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCATACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAatat < 2:191666/136‑1 (MQ=255) ttGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCTGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGc < 1:118034/136‑1 (MQ=255) ttGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCTGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGc < 1:478919/136‑1 (MQ=255) ttGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGc > 1:51000/1‑136 (MQ=255) aCCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGCACGt < 1:363890/136‑1 (MQ=255) cAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGCAc > 1:275816/1‑126 (MQ=255) cAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGCAc < 2:275816/126‑1 (MQ=255) | GCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGCACGT > NC_002947/4719775‑4720015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |