Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947699,7250AG41.9% 13.0 / 35.3 31intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (9/9);  new base G (7/6);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAG  >  NC_002947/699593‑699789
                                                                                                                                    |                                                                
tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTg                                                               >  1:355329/1‑136 (MQ=37)
tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTg                                                               >  1:92257/1‑136 (MQ=37)
tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTg                                                               <  1:324800/136‑1 (MQ=37)
 cccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGa                                                              <  1:7963/136‑1 (MQ=37)
 cccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGa                                                              <  1:410787/136‑1 (MQ=37)
  ccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa                                                             <  2:355329/136‑1 (MQ=37)
   cGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAac                                                            <  2:268255/136‑1 (MQ=37)
    gcatagCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAaca                                                           <  2:545472/131‑1 (MQ=255)
          cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGACTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGtt                                                     <  1:450003/136‑1 (MQ=17)
          cTCCACCACTATCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTATTTAAATATTCGGATATGTGATAGATATAGACTGAACACCAGtt                                                     <  1:122187/136‑1 (MQ=255)
            ccaccaCTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGTCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                                                   <  1:440506/136‑1 (MQ=21)
               ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAcc                                                <  1:401628/136‑2 (MQ=37)
                      cGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtggtg                                         <  2:329217/136‑1 (MQ=37)
                        ccGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa                                       >  2:480809/1‑136 (MQ=37)
                                aGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTa                               >  1:418970/1‑136 (MQ=37)
                                aGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACAAGTTTCACTGCTGGTGGATCAGGCTa                               >  2:317769/1‑136 (MQ=255)
                                             aaCTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                  >  1:416920/1‑136 (MQ=34)
                                              aCTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgtg                 >  1:61865/1‑136 (MQ=34)
                                               cTTAGAAATGTACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                >  1:117383/1‑136 (MQ=21)
                                               cTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                <  1:20686/136‑1 (MQ=21)
                                               cTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                >  2:107122/1‑136 (MQ=34)
                                                    aaaTGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt           <  1:289651/136‑1 (MQ=21)
                                                          aCATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa     >  2:437852/1‑136 (MQ=21)
                                                          aCATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa     >  2:440634/1‑136 (MQ=21)
                                                          aCATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa     <  2:568051/136‑1 (MQ=34)
                                                           cATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaa    >  1:360165/1‑136 (MQ=34)
                                                           cATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaa    >  1:425875/1‑136 (MQ=34)
                                                            aTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa   >  1:540205/1‑136 (MQ=21)
                                                            aTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa   >  1:384184/1‑136 (MQ=34)
                                                            aTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACAAGTTTCACTGCTGGTGGATCAGGATAAGGTAAAATTTGTGAGTTCTGCTCGaaa   >  1:304958/1‑136 (MQ=21)
                                                             ttCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAag  <  1:250583/136‑1 (MQ=34)
                                                                                                                                    |                                                                
TCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAG  >  NC_002947/699593‑699789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑