Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,856,513	A→G	D203G (GAT→GGT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,856,513	0	A	G	100.0%	114.3 / NA	31	D203G (GAT→GGT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/17);  total (14/17)

GCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAC  >  NC_002947/1856385‑1856640                                                                                                                                 |                                                                                                                                gcTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATc                                                                                                                          <  1:490919/136‑1 (MQ=255)     aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                      >  2:506214/1‑136 (MQ=255)     aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                      <  1:188419/136‑1 (MQ=255)     aTCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGt                                                                                                                      <  1:5445/136‑1 (MQ=255)             ggCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTg                                                                                                              >  1:6878/1‑136 (MQ=255)                  aatgCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAgc                                                                                                         >  2:495414/4‑135 (MQ=255)                  aaCGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGa                                                                                                         >  2:28003/1‑136 (MQ=255)                           gcgcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCtt                                                                                                <  1:354122/136‑1 (MQ=255)                           gcgcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCtt                                                                                                <  1:584407/136‑1 (MQ=255)                                 cccGGGCGGTCATGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTa                                                                                          <  1:466219/135‑1 (MQ=255)                                    gggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCt                                                                                                      >  2:502236/1‑121 (MQ=255)                                    gggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCt                                                                                                      <  1:502236/121‑1 (MQ=255)                                           cAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGccc                                                                                <  1:167131/136‑1 (MQ=255)                                           cAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGccc                                                                                <  1:168113/136‑1 (MQ=255)                                            aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCg                                                                               <  1:445089/136‑1 (MQ=255)                                            aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCg                                                                               <  2:123433/136‑1 (MQ=255)                                              gTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGAc                                                                             >  2:315285/1‑136 (MQ=255)                                                      aTGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACaa                                                                     >  1:340/1‑136 (MQ=255)                                                       tGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAg                                                                    >  2:108113/1‑136 (MQ=255)                                                        gACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGc                                                                   <  2:149848/136‑1 (MQ=255)                                                            agaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGAt                                                               >  2:204933/1‑136 (MQ=255)                                                             gaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATc                                                              <  2:213594/136‑1 (MQ=255)                                                             gaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATc                                                              <  1:315285/136‑1 (MQ=255)                                                                       cGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGt                                                    <  1:418519/136‑1 (MQ=255)                                                                               gcgTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAg                                            <  2:6878/136‑1 (MQ=255)                                                                                 gTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAgcg                                          >  1:169425/1‑136 (MQ=255)                                                                                            gACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGAcc                               >  1:40052/1‑136 (MQ=255)                                                                                            gACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGAATGAGCGGCGGCTGGAcc                               >  2:346497/1‑136 (MQ=255)                                                                                                acTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGa                           <  1:391064/135‑1 (MQ=255)                                                                                                    cgcgCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAgcgc                       >  2:373562/1‑136 (MQ=255)                                                                                                                         gggTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAc  >  1:162911/1‑136 (MQ=255)                                                                                                                                 |                                                                                                                                GCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAAC  >  NC_002947/1856385‑1856640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑