| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,011,323 | +T | intergenic (‑258/+192) | PP_0871 ← / ← SBRG_Ecoli_galM | glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,011,323 | 1 | . | T | 91.7% | 32.7 / ‑2.7 | 12 | intergenic (‑258/+192) | PP_0871/SBRG_Ecoli_galM | glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (6/5); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGT > NC_002947/1011207‑1011422 | cGGGTTGCAGGGTAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTTTTAATCGCGGTTTTGAGGGTTTTTTGTAGAGAAAAACAGCGACTTGATTT‑CTAAAATGATCTTTTAAt > 1:152327/1‑136 (MQ=255)cGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAAt > 1:383172/1‑136 (MQ=255) ggggAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATaataat < 1:422396/136‑1 (MQ=255) ggAGGGGACGGCTCCGTGGGCGTAAAGGTGCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATtc < 2:479372/136‑1 (MQ=255) tcCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGc > 2:312804/1‑136 (MQ=255) tcCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGc > 2:385932/1‑136 (MQ=255) aGGTTCCCATCTGAGGTGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCaa < 1:243892/136‑1 (MQ=255) gTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACa < 1:385932/136‑1 (MQ=255) gTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACa > 1:60064/1‑136 (MQ=255) tCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg < 1:156559/136‑1 (MQ=255) tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgtgt > 1:474036/1‑136 (MQ=255) tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgtgt > 2:120669/1‑136 (MQ=255) | CGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGA‑TG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGT > NC_002947/1011207‑1011422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |