Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,018,201	G→A	intergenic (+144/‑36)	mvaB → / → PP_mr44	hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,018,201	0	G	A	100.0%	45.8 / NA	15	intergenic (+144/‑36)	mvaB/PP_mr44	hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

TCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGGTTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACAC  >  NC_002947/4018070‑4018309                                                                                                                                    |                                                                                                              tCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGAtttt                                                                                                           >  2:403213/1‑136 (MQ=255)            tgGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGgc                                                                                                <  1:105514/136‑1 (MQ=255)              ggTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGgcac                                                                                              <  1:267516/136‑1 (MQ=255)              ggTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGgcac                                                                                              <  1:29129/136‑1 (MQ=255)              ggTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGgcac                                                                                              <  1:302483/136‑1 (MQ=255)                            tCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTatat                                                                                >  1:370920/1‑136 (MQ=255)                                  acaTTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTgg                                                                          >  1:94954/1‑136 (MQ=255)                                    aTTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTa                                                                        >  1:157067/1‑136 (MQ=255)                                                                      aaTCGGCATAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAACAAAAAAAAACGCGATACCCAATaaaaa                                      <  1:238225/136‑1 (MQ=255)                                                                      aaTCGGCAGAATTTCAGTCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAACAAAAAAAAACGCGATACCCAATaaaaa                                      <  1:235776/136‑1 (MQ=255)                                                                                            gtggtgTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATa               >  1:26191/1‑136 (MQ=255)                                                                                            gtggtgTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATa               >  2:279077/1‑136 (MQ=255)                                                                                            gtggtgTCGACCAAAAATCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATa               >  2:584383/1‑136 (MQ=255)                                                                                                        aaaaaTCTTAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGTGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAacaaca   >  1:341303/1‑136 (MQ=255)                                                                                                         aaaaTCTAAACTCATTAATTTTAAAGGATTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAacac  <  2:230671/136‑1 (MQ=255)                                                                                                                                    |                                                                                                              TCACATTGGTGTGGGTGAAGTGTTACCTCCCGCACATTTCACAGCAAAAAATCGGGTAACAGGGAAACAAATCGGCAGAATTTCAGGCTGGGTGGTGTCGACCAAAAATCTTAACTCATTAATTTTAAAGGGTTTTCAAAAGTTGGCACGCACCCTGCTATATCTCTGGTACAACAAC‑AAAAAAAACGCGATACCCAATAAAAACAACAGGTAACGGCTCTGACATAACAAGAACAACAC  >  NC_002947/4018070‑4018309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑