Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,011,323	+T	intergenic (‑258/+192)	PP_0871 ← / ← SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,011,323	1	.	T	100.0%	22.8 / NA	8	intergenic (‑258/+192)	PP_0871/SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (3/5);  total (3/5)

AACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATT  >  NC_002947/1011199‑1011440                                                                                                                              |                                                                                                                      aaCCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCTGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAatcat                                                                                                             <  1:397116/136‑1 (MQ=255)                                       gCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGcgacg                                                                       >  2:105007/1‑136 (MQ=255)                                                   ccATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCaa                                                           <  2:298813/136‑1 (MQ=255)                                                                                 tCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                             <  2:366367/136‑1 (MQ=255)                                                                                 acGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                             <  2:223529/135‑1 (MQ=255)                                                                                          tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                             >  1:79389/1‑127 (MQ=255)                                                                                          tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                             <  2:79389/127‑1 (MQ=255)                                                                                                            aaaaaCAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATAtt  >  1:208259/1‑136 (MQ=255)                                                                                                                              |                                                                                                                      AACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATATT  >  NC_002947/1011199‑1011440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑