| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,993,916 | 0 | A | C | 26.3% | 26.6 / 13.7 | 19 | intergenic (+205/‑341) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base A (11/3); new base C (5/0); total (16/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAT > NC_002947/5993828‑5994047 | aCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgggcgg > 1:199313/1‑136 (MQ=255)aCGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGGTTACCCGCGAAGCGCCCCgcgggcgg > 1:396299/1‑136 (MQ=255) aCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGa > 2:271285/1‑85 (MQ=255) aCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGa < 1:271285/85‑1 (MQ=255) acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAgg > 1:198531/1‑59 (MQ=255) acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAgg < 2:46706/59‑1 (MQ=255) acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAgg < 2:198531/59‑1 (MQ=255) acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAgg > 1:46706/1‑59 (MQ=255) aaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGaa > 2:84010/1‑136 (MQ=255) gTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGc > 2:202196/1‑136 (MQ=255) ccTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTAATGGGCGCCCAAAAACAATCGCCACGCACCAGGAAGCCCTCCTGCAATTTCCTGa > 1:387790/1‑136 (MQ=25) ataactGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGCTCTCATGGGCGCCCAAAAACTATAGCCAGGCCCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc > 1:279044/6‑136 (MQ=25) ataactGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCCAAAACTATCGCCAGGCACCTGGAAGCCCTCCTGGAATTTCCTGCCGAGCACCTcgg > 1:224639/6‑135 (MQ=21) ataactGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc > 1:166266/6‑136 (MQ=255) ataactGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATAGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc > 2:270158/6‑136 (MQ=255) ataactGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATAACCTGGGCGCCCAAAAACTATCGCCAGGCACCTGGCAGCCAACCTGCAATTTCCTGACGATCACCAcgc > 2:283576/6‑136 (MQ=25) aactGGCCCGAAACAAACGTAGGAGCGGGTTTACCAGCGAAGCGCCGCGCGGGCGGCGCGCGATCTCCTGGGCGCCCAAAAACTATCCCCAGGCCCCAGGCAGCCCTCCTGCAATTTACTGACGATAACCTcgccg > 1:277857/4‑136 (MQ=21) ggCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAt > 2:350441/1‑136 (MQ=255) ggCCAGAAACAAACGTAGGAGCGGGTTTACACGCGAAGCGCCGCGCCGCCGGCGCTCGAAAACAAGGGCGCCCAAAAAATATCGCCAGGCACCTGGAAGCCAACCTGCAATTTCCTGACGATCACCTCGCCCCCAt > 2:334359/1‑136 (MQ=25) | ACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAT > NC_002947/5993828‑5994047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |