Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	476,133	A→T	Q141L (CAG→CTG)	tsaD →	tRNA N6‑adenosine(37)‑threonylcarbamoyltransferase complex transferase subunit TsaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	476,133	0	A	T	100.0%	34.3 / NA	11	Q141L (CAG→CTG)	tsaD	tRNA N6‑adenosine(37)‑threonylcarbamoyltransferase complex transferase subunit TsaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/7);  total (4/7)

GCTGGCCTTTGCCTGGGACATCCCGGCGATCGGTGTGCACCACATGGAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCAGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGATGCTGCCGGTGAAGCGTTCGACAAGACCGCCAAGCTGATC  >  NC_002947/476002‑476230                                                                                                                                    |                                                                                                  gcTGGCCTTTGCCTGGGACATCCCGGCGATCGGTGTGCACCACATGGAGGGTCACCTGCTGGCACGCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTTGTTTCCGGTGGACATACCctgctg                                                                                               <  2:291852/136‑1 (MQ=255)              tGGGACATCCCGGCGATCGGTGTGCACCACATGGAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATg                                                                                  <  1:165127/136‑1 (MQ=255)                               cGGTGTGCACCACATGGAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGaa                                                                 <  2:200163/136‑1 (MQ=255)                               cGGTGTGCACCACATGGAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGaa                                                                 <  2:80631/136‑1 (MQ=255)                                           caTGGTGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGaa                                                     <  2:403733/136‑1 (MQ=255)                                              ggtggttCACCTGCGGGCACCCATGCTGGTAGAAAACCCGCCAGAGTTTCCGTTCGTCGGTTTGTTGGTTTCGGGTGGTCTTAGCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGc                                                  <  2:331891/130‑1 (MQ=255)                                              ggAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGACAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGc                                                  >  1:381659/1‑136 (MQ=255)                                                 gggTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTg                                               >  2:256787/1‑136 (MQ=255)                                                      aCCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGa                                          >  2:312963/1‑136 (MQ=255)                                                                aCCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGATGCTGCCGGt                                >  1:243013/1‑136 (MQ=255)                                                                                              tCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGATGCTGCCGGTGAAGCGTTCGACAAGACCGCCAAGCTGATc  <  1:131045/136‑1 (MQ=255)                                                                                              tCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCTGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGATGCTGCCGGTGAAGCGTTCGACAAGACCGCCAAGCTGATc  <  1:256787/136‑1 (MQ=255)                                                                                                                                    |                                                                                                  GCTGGCCTTTGCCTGGGACATCCCGGCGATCGGTGTGCACCACATGGAGGGTCACCTGCTGGCACCCATGCTGGAAGAAAACCCGCCAGAGTTTCCGTTCGTCGCTTTGTTGGTTTCCGGTGGACATACCCAGCTGGTTCGGGTCGATGGCATCGGTCAGTACGAACTGTTGGGCGAAAGCCTGGACGATGCTGCCGGTGAAGCGTTCGACAAGACCGCCAAGCTGATC  >  NC_002947/476002‑476230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑