Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,163,732 A→G N304D (AAC→GAC)  gtsA → mannose/glucose ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,163,7320AG99.7% 1102.4 / ‑5.4 314N304D (AAC→GAC) gtsAmannose/glucose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (133/179);  total (133/181)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 317 total aligned reads displayed.

CTGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTT  >  NC_002947/1163585‑1163878
                                                                                                                                                   |                                                                                                                                                  
cTGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTacgac                                                                                                                                                  >  2:6411010/1‑150 (MQ=255)
  ggAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTCCTGACAAGTCCTTCCTGTACGACAt                                                                                                                                                >  2:9432455/1‑150 (MQ=255)
   gAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATc                                                                                                                                               <  2:1617330/150‑1 (MQ=255)
         gccAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCg                                                                                                                                         <  1:1575050/150‑1 (MQ=255)
           cAAGGTCATTACCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGtt                                                                                                                                       <  1:8257573/150‑1 (MQ=255)
                tCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTCGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtggtgg                                                                                                                                  >  1:8208601/1‑150 (MQ=255)
                     aaCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTc                                                                                                                             >  2:7199113/1‑150 (MQ=255)
                      aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                            >  1:497479/1‑150 (MQ=255)
                      aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                            >  1:6295165/1‑150 (MQ=255)
                      aCGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCa                                                                                                                            >  1:2179487/1‑150 (MQ=255)
                       cGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCaa                                                                                                                           <  2:6618979/150‑1 (MQ=255)
                       cGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCaa                                                                                                                           <  2:1735392/150‑1 (MQ=255)
                         gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                         <  2:7641174/150‑1 (MQ=255)
                         gCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                         >  2:7750617/1‑150 (MQ=255)
                          cAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCa                                                                                                                        <  1:8774080/150‑1 (MQ=255)
                             ggCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaa                                                                                                                     >  2:8940757/1‑150 (MQ=255)
                              gCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaac                                                                                                                    >  2:5439501/1‑150 (MQ=255)
                              gCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaac                                                                                                                    >  2:2457649/1‑150 (MQ=255)
                              gCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaac                                                                                                                    >  1:34011/1‑150 (MQ=255)
                                  gCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTggtggt                                                                                                                                 >  2:957461/1‑133 (MQ=255)
                                     tGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTg                                                                                                             <  1:4348725/150‑1 (MQ=255)
                                        aGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCa                                                                                                          >  1:11590/1‑150 (MQ=255)
                                          aTCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCAcc                                                                                                        <  2:6869211/150‑1 (MQ=255)
                                              tGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctg                                                                                                    >  1:9280168/1‑150 (MQ=255)
                                              tGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctg                                                                                                    >  1:4571749/1‑150 (MQ=255)
                                               gggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgc                                                                                                   <  1:9108665/150‑1 (MQ=255)
                                                ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgct                                                                                                  <  2:5911390/150‑1 (MQ=255)
                                                ggCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgct                                                                                                  <  1:8319944/150‑1 (MQ=255)
                                                 gCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTctgctg                                                                                                 <  2:4780984/150‑1 (MQ=255)
                                                       gggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagc                                                                                           >  2:1341193/1‑150 (MQ=255)
                                                        ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaaca                                                                                                                   >  1:222739/1‑125 (MQ=255)
                                                        ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGaaca                                                                                                                   <  2:222739/125‑1 (MQ=255)
                                                        ggCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                          <  2:2434713/150‑1 (MQ=255)
                                                         gCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagcag                                                                                         >  1:1825237/1‑150 (MQ=255)
                                                         gCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagcag                                                                                         <  2:11590/150‑1 (MQ=255)
                                                         gCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagcag                                                                                         <  1:9544173/150‑1 (MQ=255)
                                                          ccAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGCGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAgg                                                                                        >  2:8781798/1‑150 (MQ=255)
                                                           cAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGa                                                                                       >  1:3768917/1‑150 (MQ=255)
                                                               agCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATc                                                                                   <  2:8255442/150‑1 (MQ=255)
                                                                          ccTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTcagca                                                                                          <  2:2330823/132‑1 (MQ=255)
                                                                          ccTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgt                                                                        >  1:7332969/1‑150 (MQ=255)
                                                                            tGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGgtgt                                                                      <  1:2942707/150‑1 (MQ=255)
                                                                              gCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                    >  2:9630714/1‑150 (MQ=255)
                                                                              gCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTg                                                                    <  1:6755388/150‑1 (MQ=255)
                                                                               cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTgg                                                                   <  2:7497006/150‑1 (MQ=255)
                                                                               cGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTgg                                                                   <  1:8556462/150‑1 (MQ=255)
                                                                                gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                  <  1:2816342/150‑1 (MQ=255)
                                                                                gaagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTggg                                                                  >  1:1027845/1‑150 (MQ=255)
                                                                                 aagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGt                                                                 <  2:3769277/150‑1 (MQ=255)
                                                                                 aagaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGt                                                                 >  2:1346012/1‑150 (MQ=255)
                                                                                  agaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGc                                                                                                                         <  2:8223554/93‑1 (MQ=255)
                                                                                  agaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTg                                                                >  2:5566650/1‑150 (MQ=255)
                                                                                  agaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGACcagcagag                                                                                       >  2:6236439/1‑125 (MQ=255)
                                                                                  agaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGACcagcagag                                                                                       <  1:6236439/127‑3 (MQ=255)
                                                                                   gaaAACTGCTGGCAAGGATTACCAGTGGGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                               <  1:3788865/150‑1 (MQ=255)
                                                                                   gaaAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTCCGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGa                                                               <  2:4325810/150‑1 (MQ=255)
                                                                                     aaaCTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAgg                                                             <  1:7489019/150‑1 (MQ=255)
                                                                                       aCTGCTGGCAAGGATTACCAGTGCGTGCCGGTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGAc                                                           <  2:1292167/150‑1 (MQ=255)
                                                                                        ctgctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACt                                                          <  1:1461121/150‑1 (MQ=255)
                                                                                          gctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTc                                                        <  1:5128012/150‑1 (MQ=255)
                                                                                          gctgGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTc                                                        <  1:8914314/150‑1 (MQ=255)
                                                                                              gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                    <  2:1958934/150‑1 (MQ=255)
                                                                                              gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                    >  1:5934015/1‑150 (MQ=255)
                                                                                              gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGa                                                    <  2:9094892/150‑1 (MQ=255)
                                                                                              gCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGACAGCAGGa                                                                                       >  2:2078509/1‑115 (MQ=255)
                                                                                               cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                   <  1:205047/150‑1 (MQ=255)
                                                                                               cAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGaa                                                   <  1:3522715/150‑1 (MQ=255)
                                                                                                aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  <  1:3216797/150‑1 (MQ=255)
                                                                                                aaGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  <  1:8106547/150‑1 (MQ=255)
                                                                                                 aGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAgg                                                 >  2:2698009/1‑150 (MQ=255)
                                                                                                  ggATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGt                                                >  1:1389211/1‑150 (MQ=255)
                                                                                                     ttACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTtgg                                                                                                                                     <  1:9601743/62‑1 (MQ=255)
                                                                                                      tACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTc                                            <  1:8639663/150‑1 (MQ=255)
                                                                                                        ccAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAg                                          >  1:1711541/1‑150 (MQ=255)
                                                                                                             gcgtgcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATca                                     <  1:263199/150‑1 (MQ=255)
                                                                                                                 gcCGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAg                                                  <  1:2325783/133‑1 (MQ=255)
                                                                                                                  ccGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAg                                >  2:8582117/1‑150 (MQ=255)
                                                                                                                   cGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAgg                               <  1:9013008/150‑1 (MQ=255)
                                                                                                                   cGTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAgg                               <  2:5805375/150‑1 (MQ=255)
                                                                                                                    gTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAggg                              >  2:7724146/1‑150 (MQ=255)
                                                                                                                    gTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAggg                              >  1:5293403/1‑150 (MQ=255)
                                                                                                                    gTTCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAggg                              >  1:4747552/1‑150 (MQ=255)
                                                                                                                     ttCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTc                                            <  1:7723174/135‑1 (MQ=255)
                                                                                                                      tCCCCGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGtt                            <  2:298245/150‑1 (MQ=255)
                                                                                                                       ccccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTc                           <  2:8141463/150‑1 (MQ=255)
                                                                                                                         ccGGTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGa                         >  2:8843874/1‑150 (MQ=255)
                                                                                                                           ggTACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATc                       <  1:7750617/150‑1 (MQ=255)
                                                                                                                             tACTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATccc                     <  2:6996472/150‑1 (MQ=255)
                                                                                                                              tcTGACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCg                    <  2:7079637/149‑1 (MQ=255)
                                                                                                                                 gACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTa                 >  1:3623140/1‑150 (MQ=255)
                                                                                                                                 gACAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTa                 >  2:3594989/1‑150 (MQ=255)
                                                                                                                                  aCAAGTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTAc                >  1:1267160/1‑150 (MQ=255)
                                                                                                                                      gTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCgg                   <  1:5667340/143‑1 (MQ=255)
                                                                                                                                      gTCCTTCCTGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCaa            <  2:3226610/150‑1 (MQ=255)
                                                                                                                                       tccttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAAc           >  1:2533121/1‑150 (MQ=255)
                                                                                                                                         cttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGa         >  1:708219/1‑150 (MQ=255)
                                                                                                                                          ttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGAc        <  1:523946/150‑1 (MQ=255)
                                                                                                                                          ttcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGAc        >  1:5008575/1‑150 (MQ=255)
                                                                                                                                           tcctGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACa       >  2:4477998/1‑150 (MQ=255)
                                                                                                                                              tGTACGACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGc    >  2:854675/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGAAGCCGCCAAGGTCATTAACGGCAAGGCCGGCATGCAGATCATGGGCGACTGGGCCAAGAGCGAATGGACCCTGGCGAAGAAAACTGCTGGCAAGGATTACCAGTGCGTGCCGTTCCCCGGTACTGACAAGTCCTTCCTGTACAACATCGACTCGTTGGTGGTGTTCAAGCAGAACAACGCTGGCACCTCTGCTGGTCAGCAGGACATCGCCCGCAAGGTGTTGGGTGAGGACTTCCAGAAGGTCTTCAGCATCAACAAGGGTTCGATCCCGGTACGCAACGACATGCTT  >  NC_002947/1163585‑1163878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑