BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,231	Δ1 bp	8.2%	intergenic (+225/+67)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,231	0	C	.	8.2%	462.1 / 37.5	147	intergenic (+225/+67)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (80/54); new base . (2/10); total (82/65)
Fisher's exact test for biased strand distribution p-value = 5.28e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 147 total aligned reads displayed.

AAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATC  >  NC_002947/5102084‑5102378

aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                     <  1:1734015/150‑1 (MQ=255)

  aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAAGGCGGGAGGTCAGGGGACAGcg                                                                                                                                                   >  2:5051086/1‑149 (MQ=255)

    tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                <  2:4947621/150‑1 (MQ=255)

       tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                             <  2:4914340/150‑1 (MQ=255)

       tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                             <  1:6114887/150‑1 (MQ=255)

       tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                             <  1:6114791/150‑1 (MQ=255)

         aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                           <  1:5936365/150‑1 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTgta                                                                                                                                         >  2:3163541/1‑148 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTGAGGGGACAGCT‑TAGCCTGaa                                                                                                                                         >  2:3163627/1‑150 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                         <  2:5148412/150‑1 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                         <  2:2275505/150‑1 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                         <  2:6264366/150‑1 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                         >  2:760574/1‑146 (MQ=255)

           tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTCCGGGGCCGGTGACAGAACAGTAACCGATGACAGACGGGTAACAATGCAATTGCGGCCGTTCCCGCGAAAGCCTGAGGGCCCGGGACAGCT‑AAGccataa                                                                                                                                         >  2:4034613/1‑146 (MQ=255)

                tGCACCGAAACGGGCCGGACTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  2:5444305/150‑1 (MQ=255)

                tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  2:244196/150‑1 (MQ=255)

                tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  1:1470676/150‑1 (MQ=255)

                tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  1:5790312/150‑1 (MQ=255)

                tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  2:4340414/150‑1 (MQ=255)

                tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                    <  2:4340418/150‑1 (MQ=255)

                   aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCa                                                                                                                                 <  2:3896620/150‑1 (MQ=255)

                        aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                                                                                                                            <  1:2381229/150‑1 (MQ=21)

                         aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                           <  1:3005047/150‑1 (MQ=21)

                         aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                           <  1:6001794/150‑1 (MQ=21)

                         aCGGGCCGGAATTGGGTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                           <  2:4766572/150‑1 (MQ=21)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                          >  2:3578442/1‑150 (MQ=255)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                          <  2:3673350/150‑1 (MQ=255)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                          <  2:6639158/150‑1 (MQ=255)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                          <  1:6425583/150‑1 (MQ=255)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                          <  1:4371051/150‑1 (MQ=21)

                          cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                          <  1:2184440/150‑1 (MQ=21)

                           gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                         >  2:5997961/1‑150 (MQ=255)

                            ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGc                                                                                                                        <  1:5015328/150‑1 (MQ=21)

                            ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGATGTAAGGGGCCAGCG‑TAGCCGGAACTGACGCAATAGCGGGc                                                                                                                        >  1:353528/1‑150 (MQ=255)

                             gCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGGGTGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCa                                                                                                                       >  1:2175859/1‑150 (MQ=255)

                              ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                      <  2:1996520/150‑1 (MQ=255)

                              ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCaa                                                                                                                      <  1:342006/150‑1 (MQ=21)

                                ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAA‑Gc                                                                                                                   >  2:1450492/1‑150 (MQ=255)

                                 gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑Gcc                                                                                                                  <  2:1870415/150‑1 (MQ=255)

                                       tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGAAGCATTCGCGGGCAA‑GCCCGCGcc                                                                                                            >  2:699231/1‑150 (MQ=255)

                                       tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCAATTCGGGAGGTGAGGGGACAGCT‑TATCCGGAACGTACGCAATCGCGGGCAA‑GCCCGCGcc                                                                                                            >  1:330212/1‑150 (MQ=255)

                                             ggCGCCCCGAACCTGGGGGGGGCCGGTGACAGAACAGTACCCGATGATTGTGGGGTAACAATGCAAACGGGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTGGCGGGCAA‑GCCCGCTCCTGCAgg                                                                                                      <  2:6738777/150‑1 (MQ=255)

                                                gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACGGACGCATTCGCGGGCCA‑GCCCGCTCCTGCAggggg                                                                                                   >  1:1125420/1‑150 (MQ=255)

                                                gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑GAGCCGGAACTTAAGCAGTCGCGGGCAA‑GCCCCCACCAGCAggggg                                                                                                   >  1:3922413/1‑150 (MQ=255)

                                                   ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGCAAGGGGGAGGTCCGGGGCCAGCG‑TAGGCTGAACAGAACCATTAGCGGGCAA‑GCCCGCGCCAGCCggggggcg                                                                                                >  1:621663/1‑147 (MQ=255)

                                                        ccTGTGCGGGGCGGGTGACAGAACATTAACCGATGATTGTCGGTTAAAAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCg                                                                                           <  2:6229551/150‑1 (MQ=39)

                                                         cgggggggggCCGGTCACACAACATTAACCTTGGATGGTCGGTTAACACTGCAAATGGGGGGTGGCCGGCTATGGCTTGTGGTCCGGGGACAGGT‑TCGCCTGAACTGACGCATCCGGGGGCAC‑CCCCGCTCCTGCAGGGGGTATTGCCgg                                                                                          <  2:2175859/144‑1 (MQ=255)

                                                            tgCGGGGCCGGTGACAGAACAGTACCCGATGTTTGGCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGAGGTCAGGGGACAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                       <  2:3952833/150‑1 (MQ=255)

                                                            tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAAAGCGTGGGGTGAGGGGACAGCT‑TTGGCGGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCGGGCGGGGGGGTTGCCggggc                                                                                       >  1:4624211/1‑148 (MQ=255)

                                                                gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTGCCGCGAATGCGTGAGGGGCGGGGGCAGCG‑TAGGCGGGACTGACGCAGTCGCGGGGAA‑GCCCCCGCCGGCCGGGGGGGTTGCCGGGGAgggg                                                                                   >  1:3626055/1‑149 (MQ=255)

                                                                ggcccGGTTAAAGAACCTTAACCTATTATTGTCGGTAAACAATGAAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTATGGGGACAGAT‑TACCCTGAACTGACGCATTCGCGGGAAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGt                                                                                   <  2:1612100/147‑1 (MQ=255)

                                                                   cgggTGACATAACATTAACCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGTGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                <  2:2740430/148‑1 (MQ=255)

                                                                   ccGGTTACACAACAGTACCCGATGATTGTCGGTTAAAAATGCAAACGGGGGCTGGCCCGCTAATGCTTGAGGTCAGGGGACAGCT‑TCGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                <  2:2740326/150‑1 (MQ=255)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCCAATTCGTGAGGTCAGGGGGCACCT‑TATCCGGAACGGACGCAATAGCGGGCCA‑CCCCCCGCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAg                                                                        >  1:111352/1‑150 (MQ=255)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGACGGGTTTACGCCGAAg                                                                        >  1:5057228/1‑150 (MQ=255)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAg                                                                        >  2:1560024/1‑150 (MQ=255)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGCAGGGGTTACGCCGAAg                                                                        >  2:6501092/1‑150 (MQ=255)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTCTTGCCGGGCAGGGTTTAAGCCGTAg                                                                        >  2:6500934/1‑150 (MQ=39)

                                                                           aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCAGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTTCCGGGAAGGGTTTAAGCCGAAg                                                                        >  2:24955/1‑150 (MQ=255)

                                                                               ctgTACCCTATGTTTGTCGGGTAACAATGCAAACGCGGGCGTGCCCGCGATTGCGTGCGTTCAGGTTACAGCT‑TCGCCTGACCTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                    <  2:1057310/148‑1 (MQ=255)

                                                                               ctgTAACCGATGATGGTCGGTTAACAATGCAAATGCGGGCTGGCCCGCGATTGCGTGTGGCCTGGGGACAGCT‑TAGCCTGAACTGAGGCATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                    <  2:1057323/148‑1 (MQ=255)

                                                                                  tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                 >  2:3837473/1‑150 (MQ=255)

                                                                                  tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAATCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                 >  2:518363/1‑150 (MQ=255)

                                                                                  tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAATGCCTGAGGTCAGGGGACACCT‑TAGCCGGAACTGACGCAATCGCCGGCAA‑GCCCCCGCCAGCACGGGGTAGTGCCCGGCAGGGTTTACGCCGAAGCCacggt                                                                 >  2:4620677/1‑146 (MQ=255)

                                                                                     ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCCCTCCTGCAGGGGGTATTGCCGGGAAGGGGTTAAGCCGCAGCCAGGTTCaa                                                              >  2:1834126/1‑149 (MQ=255)

                                                                                       gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCg                                                            >  2:1484756/1‑150 (MQ=255)

                                                                                       gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCGAAGCCAGGTTAAtag                                                            >  2:229483/1‑148 (MQ=255)

                                                                                       gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGGGGGCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCAGGTTCaaag                                                            >  2:229852/1‑147 (MQ=255)

                                                                                           ttggTCGGTAAACACTGCAACCGGGGGCTGGCCCGCTATTGGGTGCGGTCCGGGGACCGCT‑TCGCCTTACCTGCCGCATTCGGGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                        <  2:6062555/147‑1 (MQ=255)

                                                                                           atTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCCGGTTCATCGCCtt                                                        >  2:4092368/1‑150 (MQ=255)

                                                                                              gTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGTCAGCT‑TAGCCTGAACGGTCGCGTTCGCGGGCAA‑GCCCGCTGCGGCAGGGGGTGTTGCCGGGCAGGGTTTACGCCGAAGCCAGGGTAATCGCCTtgtg                                                     >  2:5357049/1‑150 (MQ=255)

                                                                                               tCGGTTAACATTGCAAACGCGGGCTTGCCCGCTAATGCGTTAGGTCTGGGTACAGAT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                    <  2:2119238/150‑1 (MQ=255)

                                                                                               tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                    >  2:6791274/1‑150 (MQ=255)

                                                                                                  gttaaataTGCAAAAGGGGGTTTGCCCGGTTTTGCTTGTGTTCGGGTTAATGAT‑TCGCCTCAAATGCCGAATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                 <  1:5711540/143‑1 (MQ=255)

                                                                                                  ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                 >  2:2202833/1‑150 (MQ=255)

                                                                                                             attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                      >  2:6105863/5‑150 (MQ=255)

                                                                                                                       ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                            >  1:966987/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  2:4581113/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:1980240/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  2:5131755/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:2707981/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:4213730/1‑150 (MQ=255)

                                                                                                                          cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:5444030/1‑150 (MQ=255)

                                                                                                                           ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  1:5949125/1‑150 (MQ=255)

                                                                                                                           ccGCGAAGCCGTGAGGTCAGGGGACCGCT‑CACCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        <  2:5318345/150‑1 (MQ=255)

                                                                                                                             gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                      >  1:1639150/1‑150 (MQ=255)

                                                                                                                             gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                      >  1:3963743/1‑150 (MQ=255)

                                                                                                                              gttttGCTTGTGGTCGGGGGCCTGCT‑TCGCCCCAAGTGCCGC‑TTCCGGGGCAACCCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                     <  1:1061555/146‑1 (MQ=255)

                                                                                                                                   gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                     >  1:550024/1‑81 (MQ=255)

                                                                                                                                   gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                     <  2:550024/81‑1 (MQ=255)

                                                                                                                                    cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa               >  2:2893058/1‑150 (MQ=255)

                                                                                                                                           tctggggACATCT‑TAGCCTTAACTGACGAATTCGCTGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCATGTGCTGTACCACACCCGTGTCAGCCAAGGtata        <  2:5054101/147‑4 (MQ=255)

                                                                                                                                           tccgggcACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga        <  1:6778825/147‑1 (MQ=255)

                                                                                                                                           tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga        >  2:6100636/1‑150 (MQ=255)

                                                                                                                                           tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga        >  1:4518366/1‑150 (MQ=255)

                                                                                                                                           tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga        >  2:2965814/1‑150 (MQ=255)

                                                                                                                                           tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCAAAGGTCga        >  2:4840930/1‑150 (MQ=255)

                                                                                                                                               gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata    >  1:1727633/1‑150 (MQ=255)

                                                                                                                                                 gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc  >  1:1855729/1‑150 (MQ=255)

                                                                                                                                                 gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc  >  2:762627/1‑150 (MQ=255)

AAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATC  >  NC_002947/5102084‑5102378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`