BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,235:1	+G	7.9%	intergenic (+229/+63)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,235	1	.	G	7.9%	649.6 / 47.9	151	intergenic (+229/+63)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base . (85/54); new base G (2/10); total (87/64)
Fisher's exact test for biased strand distribution p-value = 4.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

Only 100 of 157 total aligned reads displayed.

AATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAG  >  NC_002947/5102086‑5102384

aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGGCAGCt                                                                                                                                                         >  2:1704489/1‑150 (MQ=255)

  tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                      <  2:4947621/150‑1 (MQ=255)

     tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                   <  1:6114887/150‑1 (MQ=255)

       aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                 <  1:5936365/150‑1 (MQ=255)

       aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                 <  1:5936346/150‑1 (MQ=255)

         tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTgta                                                                                                                                               >  2:3163541/1‑148 (MQ=255)

         tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                               <  2:5148412/150‑1 (MQ=255)

         tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                               <  2:6264366/150‑1 (MQ=255)

         tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                               >  2:760574/1‑146 (MQ=255)

         tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTCCGGGGCCGGTGACAGAACAGTAACCGATGACAGACGGGTAACAATGCAATTGCGGCCGTTCCCGCGAAAGCCTGAGGGCCCGGGACAGCT‑AAGccataa                                                                                                                                               >  2:4034613/1‑146 (MQ=255)

              tGCACCGAAACGGGCCGGACTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  2:5444305/150‑1 (MQ=255)

              tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  2:4340418/150‑1 (MQ=255)

              tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  1:1470676/150‑1 (MQ=255)

              tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  1:1470480/150‑1 (MQ=255)

              tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  2:244196/150‑1 (MQ=255)

              tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                          <  2:4340414/150‑1 (MQ=255)

                 aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCa                                                                                                                                       <  2:3896620/150‑1 (MQ=255)

                 aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCa                                                                                                                                       >  2:6778825/1‑150 (MQ=255)

                      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                                                                                                                                  <  1:2381229/150‑1 (MQ=21)

                       aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                 <  1:6001794/150‑1 (MQ=21)

                       aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                 <  1:6001761/150‑1 (MQ=21)

                       aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                 <  1:3005047/150‑1 (MQ=21)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  2:6639158/150‑1 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  1:6425583/150‑1 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                >  2:3578442/1‑150 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  2:4732994/150‑1 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  2:3338779/150‑1 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  1:3416542/150‑1 (MQ=255)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                <  1:2184440/150‑1 (MQ=21)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                <  1:4371051/150‑1 (MQ=21)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                <  2:2122742/150‑1 (MQ=21)

                        cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                <  1:1706720/150‑1 (MQ=255)

                         gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                               >  2:5997961/1‑150 (MQ=255)

                          ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGATGTAAGGGGCCAGCG‑TAGCCGGAACTGACGCAATAGCGGGc                                                                                                                              >  1:353528/1‑150 (MQ=255)

                            ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                            <  2:2287833/150‑1 (MQ=255)

                              ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAA‑Gc                                                                                                                         >  2:1450492/1‑150 (MQ=255)

                               gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑Gcc                                                                                                                        <  2:1870415/150‑1 (MQ=255)

                                        ccTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGc                                                                                                               >  2:5610052/1‑150 (MQ=255)

                                          tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAg                                                                                                             >  2:738482/1‑150 (MQ=255)

                                              gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑GAGCCGGAACTTAAGCAGTCGCGGGCAA‑GCCCCCACCAGCAggggg                                                                                                         >  1:3922413/1‑150 (MQ=255)

                                                 ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGGGAGGTCAGGGGACCCCG‑TAGCCGGCACAGACGCAATCCCGGGCCA‑GCCCGCGCCAGCAGGGGGTAt                                                                                                      >  1:6162449/1‑150 (MQ=255)

                                                      ccTGTGCGGGGCGGGTGACAGAACATTAACCGATGATTGTCGGTTAAAAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCg                                                                                                 <  2:6229551/150‑1 (MQ=39)

                                                          tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAAAGCGTGGGGTGAGGGGACAGCT‑TTGGCGGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCGGGCGGGGGGGTTGCCggggc                                                                                             >  1:4624211/1‑148 (MQ=255)

                                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGGTTGCCGGGAAggg                                                                                          >  2:1893176/1‑150 (MQ=255)

                                                              gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTGCCGCGAATGCGTGAGGGGCGGGGGCAGCG‑TAGGCGGGACTGACGCAGTCGCGGGGAA‑GCCCCCGCCGGCCGGGGGGGTTGCCGGGGAgggg                                                                                         >  1:3626055/1‑149 (MQ=255)

                                                                 cgggTGACATAACATTAACCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGTGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                      <  2:2740430/148‑1 (MQ=255)

                                                                         aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCCAATTCGTGAGGTCAGGGGGCACCT‑TATCCGGAACGGACGCAATAGCGGGCCA‑CCCCCCGCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAg                                                                              >  1:111352/1‑150 (MQ=255)

                                                                         aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGACGGGTTTACGCCGAAg                                                                              >  1:5057228/1‑150 (MQ=255)

                                                                         aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAg                                                                              >  2:1560024/1‑150 (MQ=255)

                                                                         aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTCTTGCCGGGCAGGGTTTAAGCCGTAg                                                                              >  2:6500934/1‑150 (MQ=39)

                                                                             ctgTACCCTATGTTTGTCGGGTAACAATGCAAACGCGGGCGTGCCCGCGATTGCGTGCGTTCAGGTTACAGCT‑TCGCCTGACCTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                          <  2:1057310/148‑1 (MQ=255)

                                                                             ctgTAACCGATGATGGTCGGTTAACAATGCAAATGCGGGCTGGCCCGCGATTGCGTGTGGCCTGGGGACAGCT‑TAGCCTGAACTGAGGCATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                          <  2:1057323/148‑1 (MQ=255)

                                                                                tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAATCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                       >  2:518363/1‑150 (MQ=255)

                                                                                tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAATGCCTGAGGTCAGGGGACACCT‑TAGCCGGAACTGACGCAATCGCCGGCAA‑GCCCCCGCCAGCACGGGGTAGTGCCCGGCAGGGTTTACGCCGAAGCCacggt                                                                       >  2:4620677/1‑146 (MQ=255)

                                                                                   ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCCCTCCTGCAGGGGGTATTGCCGGGAAGGGGTTAAGCCGCAGCCAGGTTCaa                                                                    >  2:1834126/1‑149 (MQ=255)

                                                                                     gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAtag                                                                  >  2:933672/1‑148 (MQ=255)

                                                                                     gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCg                                                                  >  2:1484756/1‑150 (MQ=255)

                                                                                     gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCGAAGCCAGGTTAAtag                                                                  >  2:229483/1‑148 (MQ=255)

                                                                                         ttggTCGGTAAACACTGCAACCGGGGGCTGGCCCGCTATTGGGTGCGGTCCGGGGACCGCT‑TCGCCTTACCTGCCGCATTCGGGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                              <  2:6062555/147‑1 (MQ=255)

                                                                                         atTGTCGGGTACCACCCCAAACGCGGGCTGGCCCGCTATTGGGTGCGGTCAGGGGACAGCT‑TCGCCTGAACTCCCCCATTCGGGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                              <  2:2639282/150‑1 (MQ=255)

                                                                                         atTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCCGGTTCATCGCCtt                                                              >  2:4092368/1‑150 (MQ=255)

                                                                                           tGTCGGGAAACAATGAAAACGCGGGCTGGCCGGCGATTGCGTGTGTTCCGGGGACCGGT‑TCGCCTGACCTGACGAATGCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgt                                                            <  2:3922413/150‑1 (MQ=255)

                                                                                            gTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGTCAGCT‑TAGCCTGAACGGTCGCGTTCGCGGGCAA‑GCCCGCTGCGGCAGGGGGTGTTGCCGGGCAGGGTTTACGCCGAAGCCAGGGTAATCGCCTtgtg                                                           >  2:5357049/1‑150 (MQ=255)

                                                                                             tCGGTTAACATTGCAAACGCGGGCTTGCCCGCTAATGCGTTAGGTCTGGGTACAGAT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          <  2:2119238/150‑1 (MQ=255)

                                                                                             tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          >  2:6791274/1‑150 (MQ=255)

                                                                                                gttaaataTGCAAAAGGGGGTTTGCCCGGTTTTGCTTGTGTTCGGGTTAATGAT‑TCGCCTCAAATGCCGAATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                       <  1:5711540/143‑1 (MQ=255)

                                                                                                ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                       >  2:2202833/1‑150 (MQ=255)

                                                                                                           attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                            >  2:6105863/5‑150 (MQ=255)

                                                                                                           aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                            >  2:3707831/1‑150 (MQ=255)

                                                                                                                     ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                                  >  1:966987/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  1:5444030/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  1:2707981/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  2:5131755/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  1:1980240/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  1:4213730/1‑150 (MQ=255)

                                                                                                                        cccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaacaag                               >  1:4882449/1‑145 (MQ=18)

                                                                                                                         ccGCGAAGCCGTGAGGTCAGGGGACCGCT‑CACCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              <  2:5318345/150‑1 (MQ=255)

                                                                                                                          cgcgAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                             <  2:6588364/150‑1 (MQ=255)

                                                                                                                           gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                            >  1:3963743/1‑150 (MQ=255)

                                                                                                                            gttttGCTTGTGGTCGGGGGCCTGCT‑TCGCCCCAAGTGCCGC‑TTCCGGGGCAACCCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                           <  1:1061555/146‑1 (MQ=255)

                                                                                                                             gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                          >  1:2014613/1‑150 (MQ=255)

                                                                                                                                 gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                           >  1:550024/1‑81 (MQ=255)

                                                                                                                                  cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa                     >  2:2893058/1‑150 (MQ=255)

                                                                                                                                        gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg               >  2:2404806/1‑150 (MQ=255)

                                                                                                                                         tctggggACATCT‑TAGCCTTAACTGACGAATTCGCTGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCATGTGCTGTACCACACCCGTGTCAGCCAAGGtata              <  2:5054101/147‑4 (MQ=255)

                                                                                                                                         tccgggcACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              <  1:6778825/147‑1 (MQ=255)

                                                                                                                                         tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              >  2:6100636/1‑150 (MQ=255)

                                                                                                                                         tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              >  2:4841083/1‑150 (MQ=255)

                                                                                                                                         tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              >  2:2965814/1‑150 (MQ=255)

                                                                                                                                         tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCAAAGGTCga              >  2:4840930/1‑150 (MQ=255)

                                                                                                                                             gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata          >  1:1727633/1‑150 (MQ=255)

                                                                                                                                                  aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc     >  2:2506915/1‑150 (MQ=255)

                                                                                                                                                   gCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          >  1:3106697/1‑96 (MQ=255)

                                                                                                                                                   gCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          <  2:3106697/96‑1 (MQ=255)

                                                                                                                                                    cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa   >  1:5118258/1‑150 (MQ=255)

                                                                                                                                                    cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa   >  1:2864388/1‑150 (MQ=255)

                                                                                                                                                    cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa   >  1:2465942/1‑150 (MQ=255)

                                                                                                                                                     t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          >  1:446711/1‑94 (MQ=255)

                                                                                                                                                     t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                          <  2:446711/94‑1 (MQ=255)

                                                                                                                                                     t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg  >  2:4982820/1‑150 (MQ=255)

AATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAG  >  NC_002947/5102086‑5102384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`