BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,360,429:1	+T	6.0%	coding (5/264 nt)	PP_1180 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,360,429	1	.	T	6.0%	1424.5 / 79.3	334	coding (5/264 nt)	PP_1180	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (177/137); new base T (8/12); total (185/149)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 353 total aligned reads displayed.

GGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCAT  >  NC_002947/1360281‑1360578

ggcCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAatgca‑t                                                                                                                                                      <  2:3688318/150‑1 (MQ=255)

ggcCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGC‑‑GGATGCgc‑atgca‑tt                                                                                                                                                     <  1:1202338/150‑2 (MQ=255)

 gcCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAatgca‑tg                                                                                                                                                     >  2:5489419/1‑150 (MQ=255)

    tGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCGATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCC‑Tgcgc                                                                                                                                                  >  1:5914999/1‑150 (MQ=255)

       ggCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATc                                                                                                                                               <  1:2007851/150‑1 (MQ=255)

       ggCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGC‑ATGCATTGCGCATc                                                                                                                                               <  2:2731832/150‑1 (MQ=255)

        gCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGC‑ATGCATTGCGCATcc                                                                                                                                              <  1:1364533/150‑1 (MQ=255)

        gCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGC‑ATGCATTGCGCATcc                                                                                                                                              <  2:557449/150‑1 (MQ=255)

            cTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgccc                                                                                                                                          >  1:2810959/1‑150 (MQ=255)

             tGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACCGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgcccg                                                                                                                                         >  2:2224536/1‑150 (MQ=255)

             tGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgcccg                                                                                                                                         >  2:4122184/1‑150 (MQ=255)

             tGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATccgcccg                                                                                                                                         >  2:4720201/1‑150 (MQ=255)

                 ccTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGCGGATGCGC‑ATGCATTGCGCATccccgcgag                                                                                                                                       <  2:6236364/150‑8 (MQ=38)

                  cTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCgg                                                                                                                                    >  1:3104045/1‑150 (MQ=255)

                   tGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCggg                                                                                                                                   <  2:3940584/150‑1 (MQ=255)

                   tGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCggg                                                                                                                                   <  2:3731729/150‑1 (MQ=255)

                    gCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGc                                                                                                                                  >  2:4189540/1‑150 (MQ=255)

                         ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCggttatcga                                                                                                                             <  2:1726223/150‑8 (MQ=255)

                         ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTAcc                                                                                                                             <  1:3373934/150‑1 (MQ=255)

                            tcGCGAGTCGTGTCGGTTTTCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTAAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCACGCCG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGAc                                                                                                                          >  1:1853769/1‑150 (MQ=255)

                              gcgAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAatgca‑tgc                                                                                                                                                    <  1:1750146/122‑1 (MQ=255)

                              gcgAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACtt                                                                                                                        >  1:5516168/1‑150 (MQ=255)

                              gcgAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACtt                                                                                                                        >  1:6593541/1‑150 (MQ=255)

                                gAGTCGTGTCGGTTTGCAAGGGTGGGTGTCTCCACAGTTTCTATATTTTTAAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACATGCTCGATAACCACGCGG‑‑GGATGCGCAAAGCA‑TGCGCATCCGCCCCCGCGGGCGAACCGAcatag                                                                                                                      >  1:2548229/1‑146 (MQ=255)

                                   tCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGa                                                                                                                   <  1:1627177/150‑1 (MQ=255)

                                   tCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGa                                                                                                                   >  2:6830508/1‑150 (MQ=255)

                                   tCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCGTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGa                                                                                                                   <  1:4066131/150‑1 (MQ=255)

                                        tCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGAc                                                                                                              >  2:2921734/1‑150 (MQ=255)

                                           gTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCa                                                                                                           >  1:6645550/1‑150 (MQ=255)

                                             ttGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAgg                                                                                                         <  1:3782056/150‑1 (MQ=255)

                                             ttGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAgg                                                                                                         >  2:3583080/1‑150 (MQ=255)

                                                   gggtgggtGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAg                                                                                                   >  1:3598190/1‑150 (MQ=255)

                                                    ggtgggtgTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAgg                                                                                                  <  1:522315/150‑1 (MQ=255)

                                                     gtgggtgTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAggg                                                                                                 >  2:5014441/1‑150 (MQ=255)

                                                      tgggtgTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAgggg                                                                                                <  2:1318936/150‑1 (MQ=255)

                                                       gggtgTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGc                                                                                               >  2:3298567/1‑150 (MQ=255)

                                                           gtCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGACTAAGGGGCAATg                                                                                           >  2:6013399/1‑150 (MQ=255)

                                                           gtCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATg                                                                                           <  2:5154872/150‑1 (MQ=255)

                                                            tctcCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATgg                                                                                          <  1:4878177/150‑1 (MQ=255)

                                                              tcCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCt                                                                                        >  2:4365241/1‑150 (MQ=255)

                                                                cacaGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTg                                                                                      >  2:1663889/1‑150 (MQ=255)

                                                                cacaGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTg                                                                                      >  1:5539168/1‑150 (MQ=255)

                                                                cacaGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTg                                                                                      <  2:4683384/150‑1 (MQ=255)

                                                                  caGTTTCTATAGTGGTCAGGCGCCTGACTGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCACTGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGcc                                                                                    <  2:6297886/150‑1 (MQ=255)

                                                                       tCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTcc                                                                               <  1:2148624/150‑1 (MQ=255)

                                                                         tataTTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCt                                                                             >  1:3465168/1‑150 (MQ=255)

                                                                         tataTTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCt                                                                             <  1:6825853/150‑1 (MQ=255)

                                                                           tatTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTgg                                                                           >  1:6275094/1‑150 (MQ=255)

                                                                             ttGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGcc                                                                         >  2:438015/1‑150 (MQ=255)

                                                                             ttGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGcc                                                                         <  2:5302107/150‑1 (MQ=255)

                                                                                ttCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGaa                                                                      >  2:1564152/1‑150 (MQ=255)

                                                                                      cgcCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCaaa                                                                <  2:1485155/150‑1 (MQ=255)

                                                                                       gcCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAg                                                               >  2:2594344/1‑150 (MQ=255)

                                                                                       gcCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAg                                                               <  2:6555919/150‑1 (MQ=255)

                                                                                          ttATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCaa                                                                 >  1:2743588/1‑145 (MQ=255)

                                                                                          ttATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGtt                                                            >  2:4430134/1‑150 (MQ=255)

                                                                                           tATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTc                                                           >  1:3007108/1‑150 (MQ=255)

                                                                                            aTGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCt                                                          <  1:970968/150‑1 (MQ=255)

                                                                                            aTGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCt                                                          >  1:4296280/1‑150 (MQ=255)

                                                                                             tGGGGGCCCACCGTTCGGTCCACTTGCTCGTTAAGCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTg                                                         <  1:1462305/150‑1 (MQ=255)

                                                                                              gggCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTgg                                                        >  2:849247/1‑150 (MQ=255)

                                                                                                    ccACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAg                                                  >  2:1887663/1‑150 (MQ=255)

                                                                                                    ccACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAg                                                  <  1:4190825/150‑1 (MQ=255)

                                                                                                     cACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGc                                                 <  1:3309792/150‑1 (MQ=255)

                                                                                                     cACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGc                                                 <  2:4050968/150‑1 (MQ=255)

                                                                                                     cACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGc                                                 >  2:2045449/1‑150 (MQ=255)

                                                                                                     cACCGGTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGCTGCGCACTGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCCAGGGCTTGCCGCTCCCTGGCCGAAGTCACAGGTTCTGGGCCGAGc                                                 >  2:3040040/1‑150 (MQ=255)

                                                                                                          ttCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCaaa                                            <  1:4703927/150‑1 (MQ=255)

                                                                                                          ttCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCaaa                                            >  2:422410/1‑150 (MQ=255)

                                                                                                            ccGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCaaaaa                                          <  1:1543379/150‑1 (MQ=255)

                                                                                                            ccGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCaaaaa                                          <  2:1154565/150‑1 (MQ=255)

                                                                                                             cGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAAc                                         <  1:5723925/150‑1 (MQ=255)

                                                                                                                ccACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGt                                      >  1:6395889/1‑150 (MQ=255)

                                                                                                                  aCTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTg                                    >  2:4578661/1‑150 (MQ=255)

                                                                                                                   cTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGc                                   <  1:5394192/150‑1 (MQ=255)

                                                                                                                      gCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGa                                >  1:6334416/1‑150 (MQ=255)

                                                                                                                      gCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGa                                >  1:1044856/1‑150 (MQ=255)

                                                                                                                       cTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGTGACAGACTCAGTAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGAt                               <  2:2883921/150‑1 (MQ=255)

                                                                                                                         cGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATgg                             <  1:1419764/150‑1 (MQ=255)

                                                                                                                          gATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATggg                            >  1:3421187/1‑150 (MQ=255)

                                                                                                                                cTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAg                      >  2:3888634/1‑150 (MQ=255)

                                                                                                                                cTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAg                      >  1:4021409/1‑150 (MQ=255)

                                                                                                                                 tCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAgg                     >  2:4656978/1‑150 (MQ=255)

                                                                                                                                 tCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAgg                     >  1:4453595/1‑150 (MQ=255)

                                                                                                                                     gg‑‑ggATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAGTGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg                 >  2:73376/1‑150 (MQ=255)

                                                                                                                                     gg‑‑ggATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctg                 <  1:3885297/150‑1 (MQ=255)

                                                                                                                                          gATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctggcc              <  2:4821467/150‑1 (MQ=255)

                                                                                                                                           aTGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCACAAACTGTTGCTGATGGGTGCCAggcctggcct             >  2:4437593/1‑150 (MQ=255)

                                                                                                                                           aTGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctggcct             <  2:5818273/150‑1 (MQ=255)

                                                                                                                                           aTGCGC‑ATGCATTGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctggcct             >  2:2095881/1‑150 (MQ=255)

                                                                                                                                           aTGCGC‑ATGCATTGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAggcctggcct             >  1:5003252/1‑150 (MQ=255)

                                                                                                                                            tGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGgcctggcctg            <  1:2924829/150‑1 (MQ=255)

                                                                                                                                            cgcgcAATGCA‑TGCGCAGCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGgcctggcctg            <  2:6168893/149‑1 (MQ=255)

                                                                                                                                              cgcAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTcc                                                                               >  1:746462/1‑81 (MQ=255)

                                                                                                                                               gcAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCaa         >  2:1758294/1‑150 (MQ=255)

                                                                                                                                                  atgca‑tgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAacac      >  1:527220/1‑150 (MQ=255)

                                                                                                                                                  atgca‑tgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAacac      <  2:6655012/150‑1 (MQ=255)

                                                                                                                                                   tgca‑tgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACAcc     <  1:6172014/150‑1 (MQ=255)

                                                                                                                                                     ca‑tgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCa   >  1:5431502/1‑150 (MQ=255)

                                                                                                                                                      a‑tgcGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCAt  >  2:2545049/1‑150 (MQ=255)

GGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGG‑‑GGATGCGCAATGCA‑TGCGCATCCGCCCGCGCGGGCGTACCGACTTCGGGACAGACTCAGGAATAAGGGGCAATGGCTTGCCGCTCCCTGGCCGAAGTCAAAGGTTCTGGGCCGAGCCCAAAAACTGTTGCTGATGGGTGCCAGGCCTGGCCTGCAACACCCAT  >  NC_002947/1360281‑1360578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`