BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,146,499:1	+A	5.0%	coding (424/972 nt)	PP_2757 →	sugar‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,146,499	1	.	A	5.0%	1135.3 / 49.7	260	coding (424/972 nt)	PP_2757	sugar‑binding protein
Reads supporting (aligned to +/- strand): ref base . (125/122); new base A (4/9); total (129/131)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 269 total aligned reads displayed.

ATGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCC  >  NC_002947/3146350‑3146647

 tGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCCCCTCGGACACCGTCGCGGCCGGCcgcg‑c                                                                                                                                                     <  2:5275058/150‑1 (MQ=255)

    ggTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGtt                                                                                                                                                  <  1:883800/150‑1 (MQ=255)

     gTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCG‑CGGTg                                                                                                                                                 >  1:5957046/1‑150 (MQ=255)

      tAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACACCACCACCTCGGACAACG‑CGCGGCCGGCCGCGACGTTgt                                                                                                                                                <  1:5538146/150‑2 (MQ=255)

      tAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACCCCG‑CGCGGCCGGCCGCGACGTTgt                                                                                                                                                <  1:4789766/150‑2 (MQ=255)

       aaTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGc                                                                                                                                               >  2:4188098/1‑150 (MQ=255)

        aTTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGcc                                                                                                                                              <  2:4999257/150‑1 (MQ=255)

         ttCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCg                                                                                                                                             >  1:4590746/1‑150 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCCGCAGGTATCCCGCTTTTCACCCTTGATGTGCCCTCGCAGTACCGCCTCAACAACACCACCACGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGa                                                                                                                                            <  2:2199595/150‑1 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTgtc                                                                                                                                               <  1:6856048/147‑3 (MQ=255)

          tCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGa                                                                                                                                            <  1:1487352/150‑1 (MQ=255)

             gACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCACGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACCGCCTCAACAACCCCACCTCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGaggt                                                                                                                                         <  1:4443792/150‑4 (MQ=38)

                ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGACCAGCt                                                                                                                                      >  2:6746475/1‑150 (MQ=255)

                ccTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCt                                                                                                                                      >  2:5196779/1‑150 (MQ=255)

                 cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                     <  2:4533944/150‑1 (MQ=255)

                 cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                     <  2:4409099/150‑1 (MQ=255)

                 cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTg                                                                                                                                     <  2:3341603/150‑1 (MQ=255)

                  tCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTgg                                                                                                                                    <  2:2828653/150‑1 (MQ=255)

                    ggTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCTGGTAGCCCGCTTTTCACCATTGATGTGCCATCGCAGTACCGCCTCAACAACACCACCTCGGCCACCGTCGGGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTg                                                                                                                                  <  2:323024/150‑1 (MQ=255)

                     gTACCGTGAGCGGTTTCGACCCCTGGCTCAAGCGCATCCCGGCAGCAGTTATCCCGCTTTTCACCTTTTTTGTGCCATCGCAGTACCGCCACAACAACCCCCCCTCGGACACCGCCGCGGCGGGCGGCG‑CGTTGGCCGAACAGCTGGTgc                                                                                                                                 <  1:2672845/150‑1 (MQ=255)

                      tACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CTTTGGCCTAACAGCTGGTgcg                                                                                                                                <  2:2542235/150‑1 (MQ=255)

                      tACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTgcg                                                                                                                                <  2:1549624/150‑1 (MQ=255)

                       aCCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTgcgc                                                                                                                               <  2:5449767/150‑1 (MQ=255)

                           tGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGaca                                                                                                                           >  2:1489441/1‑150 (MQ=255)

                               cGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACCGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCAGGTGCGCGACACcgg                                                                                                                       >  1:2506865/1‑150 (MQ=255)

                                  gATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACcggcgg                                                                                                                    >  2:410307/1‑150 (MQ=255)

                                   aTCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACCCCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCggcggc                                                                                                                   <  2:5358502/150‑1 (MQ=255)

                                   aTCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGGATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCggcggc                                                                                                                   >  2:3827118/1‑150 (MQ=255)

                                     cGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGTTGGTGCGCGACACCGGCGGCaa                                                                                                                 >  1:1614107/1‑150 (MQ=255)

                                      gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAg                                                                                                                >  2:4654908/1‑150 (MQ=255)

                                       aCCCCTGGCTCACGCGCATCCAGGCTGCAGGTATCCCGATTTTCACATTTGTTGTGCCATCGCAGTACAGCATCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAgg                                                                                                               <  1:4775768/150‑1 (MQ=255)

                                       aCCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAgg                                                                                                               >  1:2788321/1‑150 (MQ=255)

                                        ccccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAggg                                                                                                              <  2:3292475/150‑1 (MQ=255)

                                         cccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGc                                                                                                             >  2:3999064/1‑150 (MQ=255)

                                          cgtGGCTCATGCGCATCCTGGCAGCTGGTATCCCGCTTTTCACCATTGTTGTGCCATCGCAGTACAGCCTCAACAACACCCCCTCGCACAACGGCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGcc                                                                                                            <  1:6104075/148‑1 (MQ=255)

                                          ccTGGCTCACGCGCACCCCGGCCGCCGGTACCCCGCTTTACACCATTGATGTGCAATCGCAGTCCAGCCTCAACAACCCCACCCCGGCCACCGCCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGcc                                                                                                            <  1:1045803/150‑1 (MQ=255)

                                            tGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGc                                                                                                                                               <  1:2133084/113‑1 (MQ=255)

                                                tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATcc                                                                                                      >  2:5935558/1‑150 (MQ=255)

                                                  acgcgcATCCAGGCAGCAGGTAGCCCGCTTTTCACCCTTGCTGTGCCATCGCAGTACAGCCTCACCAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTg                                                                                                    <  2:6398618/148‑1 (MQ=255)

                                                  aaGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTg                                                                                                    >  2:1978141/1‑150 (MQ=255)

                                                   cgcgcATCCAGGCAGCCGGTATCCCGCTTTTCACCATTTATGTGCCAGCGCCGTACAGCCTCAACAACCCCACCGCGGACACCGGCGGGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTgg                                                                                                   <  1:4265132/149‑1 (MQ=255)

                                                    gcgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                  >  2:387156/1‑150 (MQ=255)

                                                    gcgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgt                                                                                                  >  1:4985705/1‑150 (MQ=255)

                                                       cATCCTGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGtt                                                                                               <  2:1255019/150‑1 (MQ=255)

                                                         ccacggCAGCAGTTACCCCGCTTTTCACCTTTGATGTCCCATCGCAGTACCGCCACAACAACCCCCCCGCGCACCCCGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgtgtcgc                                                                                             <  1:2575581/146‑4 (MQ=255)

                                                           ctggCCGCCGGTATCCCGATTTCCACCTTTTTGGTCCCATCGCACAACACCACCAACACCCCCCCCCCGCACACCGGCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAAc                                                                                           <  1:2027538/148‑1 (MQ=255)

                                                            cggCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCACCACCACCCCCTCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACg                                                                                          <  2:1707957/149‑1 (MQ=255)

                                                            aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACg                                                                                          >  2:2116825/1‑150 (MQ=255)

                                                                    ggTATCCCGCTTTTCACCATTGATGTGCCATCGCTGTACAGCCTCAACAACACCACCGCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTAc                                                                                  <  1:5691587/150‑1 (MQ=255)

                                                                    ggTATCCCGATTTACACCATTGATGGGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTAc                                                                                  <  1:2226185/150‑1 (MQ=255)

                                                                       aTCCCGCTTTTCACCATTGATGTGCCATCGCCGTACCGCCTCACCAACACCCCCTCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACggg                                                                               <  2:6305443/150‑1 (MQ=255)

                                                                          ccGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTg                                                                            >  2:1154919/1‑150 (MQ=255)

                                                                          ccGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCCGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCGTCTACgggggg                                                                            >  1:6518543/1‑148 (MQ=255)

                                                                          ccGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCCTCCTGGTGTTCAACGGCTTCTACGGGGTg                                                                            >  1:5494273/1‑150 (MQ=255)

                                                                                 tCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCAGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTTCGGGGTGCCGGTCt                                                                     >  1:5734710/1‑150 (MQ=255)

                                                                                 tCACCATTGAGGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCt                                                                     <  2:390671/150‑1 (MQ=255)

                                                                                   aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                   >  2:470083/1‑150 (MQ=255)

                                                                                   aCCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                                   <  2:580974/150‑1 (MQ=255)

                                                                                    ccATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                                                  <  2:6253710/150‑1 (MQ=255)

                                                                                     cATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGc                                                                 >  2:4831268/1‑150 (MQ=255)

                                                                                      aTTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCg                                                                >  2:3532206/1‑150 (MQ=255)

                                                                                         taTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATc                                                             <  2:4309438/149‑1 (MQ=255)

                                                                                         gttgtcCCATCGCAGTACCCCCACAACAACCCCACCTCGGACACCGGGGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATc                                                             <  1:3643619/148‑1 (MQ=255)

                                                                                         gATGTGCCCTCGCAGTACAGCCTCCACACCCCCACCTCGGACACCGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATc                                                             <  2:4199234/150‑1 (MQ=255)

                                                                                          aTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATcc                                                            >  1:5222389/1‑150 (MQ=255)

                                                                                           tgtgCCATCGCCGTACACCCACAACACCCCCCCCCCGCACAACGGCGCGGCCGGCCGCG‑CGTTGGCCGCACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCg                                                           <  1:1270628/150‑1 (MQ=255)

                                                                                            ggcccATCCCAGTACCCCCACACCACCACCCCCGCGCACACCCGCGCGCCCGGCCGCG‑CGTGGGCCCAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGc                                                          <  1:3051435/147‑1 (MQ=255)

                                                                                               ccATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTgg                                                                                                                                                <  1:457461/61‑1 (MQ=255)

                                                                                                    gCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTAcgacga                                                  <  2:1778940/150‑1 (MQ=255)

                                                                                                        tACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                              >  2:584244/1‑150 (MQ=255)

                                                                                                          cAGCCTCAACAACACCCCCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaa                                            <  2:4227393/150‑1 (MQ=255)

                                                                                                          cAGCCTCAACAACACCCCCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaa                                            <  2:728109/150‑1 (MQ=255)

                                                                                                          cAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaa                                            >  1:3909901/1‑150 (MQ=255)

                                                                                                           aGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaag                                           >  2:2975555/1‑150 (MQ=255)

                                                                                                            gCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaaga                                          <  2:4955305/150‑1 (MQ=255)

                                                                                                             ccTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                         <  2:2109049/150‑1 (MQ=255)

                                                                                                              cacaacacCCCCCCCGCGGACACCGGCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                        <  1:5196779/148‑1 (MQ=255)

                                                                                                              cTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                        >  2:615357/1‑150 (MQ=255)

                                                                                                              cTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                        >  2:3022281/1‑150 (MQ=255)

                                                                                                              cTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGaagaag                                        >  2:1605788/1‑150 (MQ=255)

                                                                                                               tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAgg                                       >  2:1088569/1‑150 (MQ=255)

                                                                                                               tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAgg                                       >  2:1037119/1‑150 (MQ=255)

                                                                                                               tCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCAGCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGCGTTGAAGAAgg                                       >  1:5134502/1‑150 (MQ=255)

                                                                                                                  acaacaCCACCTCGGACAACGTCGCGGCCGGCCGCC‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGGGTTCAACGGCGTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTAc                                    >  1:3998526/1‑150 (MQ=255)

                                                                                                                       accaccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGcc                               >  2:3650021/1‑150 (MQ=255)

                                                                                                                         caccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGa                             >  1:5152674/1‑150 (MQ=255)

                                                                                                                         caccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCAACGACGAGTTGAAGAAGGTACTGGCCGa                             >  1:2963507/1‑150 (MQ=255)

                                                                                                                           ccTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatc                           <  2:4590746/150‑1 (MQ=255)

                                                                                                                            ttCGGCCAACG‑CGCGGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatca                          >  1:4192191/2‑150 (MQ=39)

                                                                                                                             tCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGGGTTCAACGGCTTCTACGGGGTGCAGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat                         >  2:3076354/1‑150 (MQ=255)

                                                                                                                               ggACACCGGCGCGGCCGGCCGCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATcc                       <  2:4284995/150‑1 (MQ=255)

                                                                                                                               ggACAACGGCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATcc                       <  2:516602/150‑1 (MQ=255)

                                                                                                                                 aCAACGCCGCGGCCGGCCGCG‑CTTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTTTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGc                     <  1:5875032/150‑1 (MQ=255)

                                                                                                                                       gcgcgGCCGGCCGCGACGTTGTCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTg                >  1:6285054/2‑150 (MQ=255)

                                                                                                                                       gcgcgGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGc               <  1:4712794/149‑1 (MQ=255)

                                                                                                                                        cgcgGCGGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCa              <  1:3442230/150‑1 (MQ=255)

                                                                                                                                           ggccggccGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAt           >  1:4132046/1‑150 (MQ=255)

                                                                                                                                           ggccggccGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAt           >  2:6377675/1‑150 (MQ=255)

                                                                                                                                            gccggccgCG‑CGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATc          <  1:101101/150‑1 (MQ=255)

                                                                                                                                                   gcg‑cgTTGACCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatc                           >  1:809101/1‑126 (MQ=255)

ATGCGGTAATTCAGACCCTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCG‑CGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGATCGTCCAGCC  >  NC_002947/3146350‑3146647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`