BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,497,082	A→T	9.0%	L110Q (CTG→CAG)	PP_3096 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,497,082	0	A	T	9.0%	619.8 / 21.5	220	L110Q (CTG→CAG)	PP_3096	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (115/76); new base T (5/18); total (121/99)
Fisher's exact test for biased strand distribution p-value = 6.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

CGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCA  >  NC_002947/3496933‑3497229

cGACGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTGTGCCAGGACTCGTAGAACCACGGGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCTGCATCGTCTCGCTACTGCACGTGGCGCTCGagcagca                                                                                                                                                     <  2:1464640/150‑1 (MQ=255)

cGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGagcagca                                                                                                                                                     <  1:100996/150‑1 (MQ=255)

  aaGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGagcagcagc                                                                                                                                                   <  1:5645065/150‑1 (MQ=255)

    gCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGGGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTc                                                                                                                                                 >  1:4855696/1‑150 (MQ=255)

     cccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCg                                                                                                                                                <  1:834603/150‑1 (MQ=255)

     cccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCg                                                                                                                                                <  1:4329575/150‑1 (MQ=255)

     cccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCg                                                                                                                                                <  1:4193989/150‑1 (MQ=255)

      ccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCTGCTGCTCga                                                                                                                                               <  1:3061210/150‑2 (MQ=255)

      ccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCgg                                                                                                                                               >  1:1380462/1‑150 (MQ=255)

      ccGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCgg                                                                                                                                               >  1:1430003/1‑150 (MQ=255)

       cGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGgt                                                                                                                                              <  2:2772147/150‑1 (MQ=255)

             gCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACgg                                                                                                                                        <  2:4611779/150‑1 (MQ=255)

               cATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGc                                                                                                                                      <  1:2694109/150‑1 (MQ=255)

                aTTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCg                                                                                                                                     >  2:4436988/1‑150 (MQ=255)

                 ttGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGt                                                                                                                                    <  1:6871774/150‑1 (MQ=255)

                     gcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgca                                                                                                                                >  2:1800741/1‑150 (MQ=255)

                     gcTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgca                                                                                                                                >  2:3648141/1‑150 (MQ=255)

                      cTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTgctgc                                                                                                                               <  1:477447/150‑5 (MQ=255)

                      cTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgcag                                                                                                                               >  2:122929/1‑150 (MQ=255)

                       tCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgcagc                                                                                                                              >  1:3850076/1‑150 (MQ=255)

                       tCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgcagc                                                                                                                              >  1:1652683/1‑150 (MQ=255)

                       tCGTGCTCGATGTGGAATTTGAATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGgcagc                                                                                                                              >  2:5474590/1‑150 (MQ=255)

                          tGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACg                                                                                                                           >  1:5038465/1‑150 (MQ=255)

                               gATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGa                                                                                                                      >  1:4061111/1‑150 (MQ=255)

                                aTGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCCTGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGCTGCCACGCCCgtt                                                                                                                     <  2:3316311/150‑3 (MQ=255)

                                aTGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGAc                                                                                                                     <  2:173520/150‑1 (MQ=255)

                                  gtgGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGCTGCCACGCCCgtaca                                                                                                                   <  2:6847336/150‑5 (MQ=255)

                                   tgGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGc                                                                                                                  >  2:10053/1‑150 (MQ=255)

                                   tgGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGc                                                                                                                  >  2:6591626/1‑150 (MQ=255)

                                       aTTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACg                                                                                                                                         >  1:3469646/1‑123 (MQ=255)

                                        tttGTTTTTTTGCCAGGCCTCTTAGAACACGGTGGTCTTGGGGTGCGAGATGTTTTCGAGAAAGGCTTGGGGGGCATGGTCGCTTTACTGCAGGTGGGGCTGGTGGGGCAGGTGGGTTTCGGGGGGTGGCAGCACGCCCGACGGCCCGAc                                                                                                             <  2:5846521/150‑1 (MQ=255)

                                         ttGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGTGAAGTTGTCGAGAAACGCTTGGGGGGCATCGTCGCTATACTGCAGGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGAcc                                                                                                            <  2:5130202/150‑1 (MQ=255)

                                          tGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCa                                                                                                           >  2:5191200/1‑150 (MQ=255)

                                           gTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAg                                                                                                          >  1:5931699/1‑150 (MQ=255)

                                             aTTTTGCCCAGGCCTCGTAGACCACCGGGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATCCTGCACGTGGCGCTCGCGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGc                                                                                                        <  2:2217262/150‑1 (MQ=255)

                                                tttGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCa                                                                                                     >  2:1089553/1‑150 (MQ=255)

                                                    ccAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGaa                                                                                                 >  2:5981153/1‑150 (MQ=255)

                                                     cAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATCCTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAg                                                                                                <  2:4552551/150‑1 (MQ=255)

                                                     cAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAg                                                                                                >  2:5045146/1‑150 (MQ=255)

                                                      aGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAgg                                                                                               >  2:6602176/1‑150 (MQ=255)

                                                       ggCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGt                                                                                              >  2:3602789/1‑150 (MQ=255)

                                                        gCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTg                                                                                             >  1:2972954/1‑150 (MQ=255)

                                                           tCGTAGAACACCGTGGTCATGGGGTGCGATATGATGTCGAGAAACGATGGGGCGGCATCGTCGCGATCCTGCAGGTGGCGCTCGAGCAGCAGCTGGGTGTCGGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGAcc                                                                                          <  1:4556241/150‑1 (MQ=255)

                                                           tCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGAcc                                                                                          >  2:3963417/1‑150 (MQ=255)

                                                           tCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGAcc                                                                                          >  1:3955695/1‑150 (MQ=255)

                                                           tCGTAGAACAACGTGGCCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGAcc                                                                                          >  1:5299739/1‑150 (MQ=255)

                                                                 aacaacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACt                                                                                    >  1:4704630/1‑150 (MQ=255)

                                                                 aacaacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACt                                                                                    >  1:518466/1‑150 (MQ=255)

                                                                    aacGTGGTCATGCGGTGCGAGAAGATGTCTAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCCGCTCGGTGTCCGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTcc                                                                                 <  2:4977767/150‑1 (MQ=255)

                                                                    aacGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCAGGAAGGTGACCTGCACTTcc                                                                                 >  2:5539038/1‑150 (MQ=255)

                                                                    aacGGGGTCATGGGGTGCGTGAAGTGGTCGATAAACGCATGGGCGGCATCGTCGCGATACGGCAGGTGGCGCTCGAGCAGCTGCGCGGTGTAGGGGCGTGGAAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTcc                                                                                 <  2:5390708/150‑1 (MQ=255)

                                                                               gCGGTGCTAGAAGATGTCGAGAACCGCATGGGCGGCATCGTCGCGCTACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTCCGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCaa                                                                      <  2:1595706/150‑1 (MQ=255)

                                                                               gCGGTGCCACAATATTTCCACAAACGCATGGGCGGCATCGTCGCTATACTCCACGTGGCCATCTACAACAAGCTCGTTTTACGGGCGTGGAACCACCCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCaa                                                                      <  2:1302503/150‑1 (MQ=255)

                                                                                  gTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgcc                                                                   >  2:6131777/1‑150 (MQ=255)

                                                                                   tGCGATAAGATTTCGAGAAACGCTTGGGCGGAATCGTCGCGATACTGCAGGTGGCGCTCGAGCAGCAGGGCGGTTTCCGGGGGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccg                                                                  <  2:6076012/150‑1 (MQ=255)

                                                                                   tGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccg                                                                  >  1:5722737/1‑150 (MQ=255)

                                                                                      gagaAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccggcc                                                               >  1:1353339/1‑150 (MQ=255)

                                                                                      gagaAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccggcc                                                               >  1:6431026/1‑150 (MQ=255)

                                                                                       ataagtTGTCGAGAAACGCTTGGGCGGAATCGGCGCTATACTGCAGGTGGCGCTCGCGCAGCTGCTGGGTGTAGGGGGGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAgccggccg                                                              <  1:2190555/148‑1 (MQ=255)

                                                                                            tggTCGTGAAAGGCTGGGGCGGCTTCGTGGCTATACTGCCGGGGGCGCTCGAGCCGCCGCTGGGTGTCGGGGGGGGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGac                                                         <  1:5504250/148‑1 (MQ=255)

                                                                                            aTGCCGAGAAACGCTTGGGCGGCATCGTCGCGATACTGCACGGGGCGCTCGAGCAGCAGCTGGGTGTAGGGGCGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGac                                                         <  2:3227220/150‑1 (MQ=255)

                                                                                             tGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGaca                                                        >  1:4686249/1‑150 (MQ=255)

                                                                                               tCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACAcc                                                      >  1:580104/1‑150 (MQ=255)

                                                                                               tCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCAGGCCGGCGACAcc                                                      >  1:4671757/1‑150 (MQ=255)

                                                                                                 tccaaccGCTTGGGGGGAATCGTGGCTATCGTGCCCGTGGCGCTCGAGCCGCAGCTGGGTGTCGGGGGGTGGCACCCCGCCCCACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    <  1:1239059/144‑1 (MQ=255)

                                                                                                 gagaAACGCTTGGGGGGCATGGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    <  2:4873041/150‑1 (MQ=255)

                                                                                                 gagaAACGCTTGGGCGGCATCGTCGCTATACTGCACGTGGCGCTCGAGCAGCAGCTGGTTGTAGGGGCGGGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    <  2:5794403/150‑1 (MQ=255)

                                                                                                 gagaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCGGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    >  2:2056889/1‑150 (MQ=255)

                                                                                                 gagaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    >  1:2075897/1‑150 (MQ=255)

                                                                                                 gagaAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCtt                                                    >  1:1555991/1‑150 (MQ=255)

                                                                                                       cgctggggCGGCATCGTGGCGATACTGCACGTGGCGCTGGAGCAGCTGCTGGGTGTCCGGGCGTGGCTGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTc                                              <  1:6297352/145‑1 (MQ=255)

                                                                                                        gCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcg                                             >  1:858739/1‑150 (MQ=255)

                                                                                                        gCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcg                                             >  2:2936302/1‑150 (MQ=255)

                                                                                                        gCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCAATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcg                                             <  2:3376270/150‑1 (MQ=255)

                                                                                                        gCATGGGCGGCATCGTCGCGACACGGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCAATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcg                                             <  2:1115751/150‑1 (MQ=255)

                                                                                                         cttGGGCGGAATCGTCGCGATACTGCAGGTGGCGCTCGAGCAGCAGCTGGGTGTACGGGCGTGGAAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcgc                                            <  2:3192872/148‑1 (MQ=255)

                                                                                                         ctgggggggCAGGGTCGCTATACTGCCGGTGGCGCTGGAGCAGCTGCTGGGTTTTGGGGGGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcgc                                            <  1:2572436/147‑1 (MQ=255)

                                                                                                         cATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTcgc                                            >  2:6501207/1‑150 (MQ=255)

                                                                                                         cATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGACGGCGACACCTTGCCTTcgc                                            >  2:1947379/1‑150 (MQ=255)

                                                                                                                ggcATCGTCGCGATACGGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGGGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                     <  2:5239410/150‑1 (MQ=255)

                                                                                                                ggcATCGGCGCGATACTGCAGGTGGCGCTCGAGAAGCTGCTCGGTGTCCGGGCGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                     <  1:6745930/150‑1 (MQ=255)

                                                                                                                ggaaTCGTCGCGTTACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGaa                                     <  2:4542884/147‑1 (MQ=255)

                                                                                                                  cATGGTCGCGATACTGCACGGGGCGCTCGAGCAGCAGCTGGGTGTACGGGCGTGGCTGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGt                                   <  1:6244066/150‑1 (MQ=255)

                                                                                                                  cATCGTCGCGATACTGCAGGTGGCGCTCGAGCAGCAGGTGGGTGTCCGGGCGTGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGt                                   <  1:1056316/150‑1 (MQ=255)

                                                                                                                      gtcgCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAg                               >  1:6687409/1‑150 (MQ=255)

                                                                                                                      gtcgCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAg                               >  1:199585/1‑150 (MQ=255)

                                                                                                                           gATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGc                          >  2:2992173/1‑150 (MQ=255)

                                                                                                                                  cACGTGGCGCTCGAGCAGCAGCTCGGGGTCCGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTc                   <  2:4772186/150‑1 (MQ=255)

                                                                                                                                   aCGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCg                  <  2:3740356/150‑1 (MQ=255)

                                                                                                                                    cGTGGCGCTCGAGCAGCTGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGc                 <  2:5599874/150‑1 (MQ=255)

                                                                                                                                      tGGCGCTCGAGCAGCAGCTCGGTGTAGGGGGGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTg               <  2:3706194/150‑1 (MQ=255)

                                                                                                                                      tGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTg               <  2:6751107/150‑1 (MQ=255)

                                                                                                                                         ggcTCGAGCCGCTGCTGGGTGTCGGGGGGGGGCAGCCCGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGcc            <  1:2394278/149‑1 (MQ=255)

                                                                                                                                           cTCGAGCAGCTGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCgg          <  2:4201491/150‑1 (MQ=255)

                                                                                                                                           cTCGAGCAGCTGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCACTCGAAGTGCAGGTCGCTGACCTCGCTGGCCgg          <  2:6392359/150‑1 (MQ=255)

                                                                                                                                           accGAGCTGCTGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCgg          >  1:1539408/3‑150 (MQ=255)

                                                                                                                                              gAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCCTGAAGGTGACCTGCACTTCCGACAGCGGCACGCCGGCCGGCGACACCTTGCCTTCGCGCTCGACGTGCAGGTCGCTGACCTCGCTGGCCGGGac       >  2:4059555/1‑149 (MQ=255)

                                                                                                                                                gcagcagcTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGACCg     >  1:6471134/1‑150 (MQ=255)

                                                                                                                                                   gcagcTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCa  <  2:2801406/150‑1 (MQ=255)

                                                                                                                                                   gcagcTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCAGGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCa  >  2:3601117/1‑150 (MQ=255)

CGAAGCCCGAGGTGCCATTGCGCTCGTGCTCGATGTGGAATTTGTATTTTTGCCAGGCCTCGTAGAACAACGTGGTCATGCGGTGCGAGAAGATGTCGAGAAACGCATGGGCGGCATCGTCGCGATACTGCACGTGGCGCTCGAGCAGCAGCTCGGTGTACGGGCGTGGCAGCACGCCCGACGGCCCGACCAGGCCCATGAAGGTGACCTGCACTTCCGACAGCGGCAAGCCGGCCGGCGACACCTTGCCTTCGCGCTCGAAGTGCAGGTCGCTGACCTCGCTGGCCGGGAACGCCA  >  NC_002947/3496933‑3497229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`