BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,612,408	A→T	5.1%	K29M (AAG→ATG) ‡	PP_4079 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,612,408	0	A	T	5.1%	1035.5 / 27.8	303	K29M (AAG→ATG) ‡	PP_4079	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (153/134); new base T (6/10); total (159/144)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 306 total aligned reads displayed.

GACCAGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCAGCC  >  NC_002947/4612260‑4612557

gACCAGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAg                                                                                                                                                      >  1:4829299/1‑150 (MQ=255)

    aGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTgg                                                                                                                                                  <  2:4719875/150‑1 (MQ=255)

    aGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTgg                                                                                                                                                  <  2:2870860/150‑1 (MQ=255)

      cTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGgcg                                                                                                                                                <  2:1849079/150‑1 (MQ=255)

          gtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgc                                                                                                                                            <  2:2525657/150‑2 (MQ=255)

          gtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgc                                                                                                                                            <  2:2222553/150‑2 (MQ=255)

          gtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgc                                                                                                                                            <  1:3959404/150‑2 (MQ=255)

          gtcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTgg                                                                                                                                            >  1:3806962/1‑150 (MQ=255)

           tcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGa                                                                                                                                           <  2:1690268/150‑1 (MQ=255)

           tcgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGa                                                                                                                                           <  1:2497605/150‑1 (MQ=255)

            cgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGAc                                                                                                                                          <  2:1126518/150‑1 (MQ=255)

            cgATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCACTGGAc                                                                                                                                          <  2:832947/150‑1 (MQ=255)

             gATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGCGCCACCTTGGTGGCTCTgctca                                                                                                                                         <  2:5585439/150‑5 (MQ=255)

                gACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACt                                                                                                                                      <  1:1326661/150‑1 (MQ=255)

                gACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACt                                                                                                                                      <  1:297845/150‑1 (MQ=255)

                   cACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   >  1:1122400/1‑150 (MQ=255)

                   cACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGt                                                                                                                                   >  1:5504350/1‑150 (MQ=255)

                    aCGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGtt                                                                                                                                  <  1:6266855/150‑1 (MQ=255)

                     cGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTa                                                                                                                                 <  2:722944/150‑1 (MQ=255)

                      gATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTAc                                                                                                                                <  2:5363638/150‑1 (MQ=255)

                        tGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCa                                                                                                                              >  1:5425620/1‑150 (MQ=255)

                           ccAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATc                                                                                                                           >  1:72537/1‑150 (MQ=255)

                            cAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCg                                                                                                                          >  1:3599530/1‑150 (MQ=255)

                                   gTCCCGTTGGCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGgccgcc                                                                                                                   <  2:5757938/150‑1 (MQ=255)

                                    tCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGccc                                                                                                                  >  2:1254909/1‑150 (MQ=255)

                                     cccGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCa                                                                                                                 <  2:6209003/150‑1 (MQ=255)

                                       cGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAg                                                                                                               >  1:5064605/1‑150 (MQ=255)

                                            tCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaa                                                                                                          <  2:3807279/150‑1 (MQ=255)

                                             ccGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaac                                                                                                         <  2:6761661/150‑1 (MQ=255)

                                                 ttCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaacccaa                                                                                                     >  2:5061985/1‑150 (MQ=255)

                                                 ttCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGAcaacccaa                                                                                                     >  2:607951/1‑150 (MQ=255)

                                                      ggggCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAAcccaacccaa                                                                                                <  2:3764085/150‑1 (MQ=255)

                                                        ggCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGt                                                                                              <  1:3459539/150‑1 (MQ=255)

                                                             tCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGt                                                                                         >  2:4308365/1‑150 (MQ=255)

                                                               aTGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTc                                                                                       <  2:4902460/150‑1 (MQ=255)

                                                                tGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCa                                                                                      <  1:3019699/150‑1 (MQ=255)

                                                                  aGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGc                                                                                    <  2:366371/150‑1 (MQ=255)

                                                                     cgccTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATc                                                                                 <  2:6334921/150‑1 (MQ=255)

                                                                         tGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACg                                                                             >  2:2459727/1‑150 (MQ=255)

                                                                           gCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTc                                                                           >  2:1002350/1‑150 (MQ=255)

                                                                              gTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGc                                                                        >  1:6105462/1‑150 (MQ=255)

                                                                              gTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGc                                                                        >  2:1980561/1‑150 (MQ=255)

                                                                                cTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGccc                                                                      >  1:3905723/1‑150 (MQ=255)

                                                                                  ggATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGGCCTTGAGCAGAGCCACCCAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCACGTCGCGTTCAGCATCAACGTCAGCCCaa                                                                    <  1:372303/150‑1 (MQ=255)

                                                                                     ttGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTTGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGc                                                                 >  1:4688636/1‑150 (MQ=255)

                                                                                     ttGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGc                                                                 >  2:3539682/1‑150 (MQ=255)

                                                                                       gcgcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTc                                                               <  2:3176199/150‑1 (MQ=255)

                                                                                        cgcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCa                                                              <  1:1011624/150‑1 (MQ=255)

                                                                                        cgcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGT‑CTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCaa                                                             <  2:1436085/150‑1 (MQ=255)

                                                                                         gcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCaa                                                             <  1:6386004/150‑1 (MQ=255)

                                                                                         gcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCaa                                                             >  2:1589263/1‑150 (MQ=255)

                                                                                         gcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCaa                                                             >  2:6016317/1‑150 (MQ=255)

                                                                                         gcCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCaa                                                             <  1:2738039/150‑1 (MQ=255)

                                                                                          ccTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAAc                                                            <  1:501303/150‑1 (MQ=255)

                                                                                           ctctGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACa                                                           >  2:4585972/1‑150 (MQ=255)

                                                                                           ctctGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACa                                                           <  1:4632742/150‑1 (MQ=255)

                                                                                            tctGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAg                                                          >  2:4342724/1‑150 (MQ=255)

                                                                                            tctGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAg                                                          >  2:5101219/1‑150 (MQ=255)

                                                                                                ctggtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCacc                                                      >  2:4847230/1‑148 (MQ=255)

                                                                                                ctggtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAAc                                                      >  1:1945698/1‑150 (MQ=255)

                                                                                                ctggtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAAc                                                      >  1:6719577/1‑150 (MQ=255)

                                                                                                 tggtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAAcc                                                     <  2:6501095/150‑1 (MQ=255)

                                                                                                   gtcgtgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCg                                                   >  2:5917040/1‑150 (MQ=255)

                                                                                                       tgctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACa                                               >  2:3424601/1‑150 (MQ=255)

                                                                                                        gctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAg                                              >  2:2289784/1‑150 (MQ=255)

                                                                                                         ctggtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAgt                                             <  1:6202939/150‑1 (MQ=255)

                                                                                                            gtcgGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTc                                          <  2:4472537/150‑1 (MQ=255)

                                                                                                               ggACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGAc                                       >  1:1483016/1‑150 (MQ=255)

                                                                                                               ggACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGAc                                       >  1:704181/1‑150 (MQ=255)

                                                                                                                     ccAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggcg                                 >  2:5209385/1‑150 (MQ=255)

                                                                                                                      cAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggcgg                                >  1:2235163/1‑150 (MQ=255)

                                                                                                                      cAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggcgg                                >  2:5252139/1‑150 (MQ=255)

                                                                                                                      cAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTggcgg                                >  2:4820558/1‑150 (MQ=255)

                                                                                                                         gTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCa                             >  1:4054591/1‑150 (MQ=255)

                                                                                                                           tGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATc                           <  1:3150987/150‑1 (MQ=255)

                                                                                                                               cGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTg                       >  2:1164787/1‑150 (MQ=255)

                                                                                                                                gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  2:3599530/150‑1 (MQ=255)

                                                                                                                                gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  1:2784255/150‑1 (MQ=255)

                                                                                                                                gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      >  1:5653268/1‑150 (MQ=255)

                                                                                                                                gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      <  2:5832310/150‑1 (MQ=255)

                                                                                                                                gTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTga                      >  1:6325399/1‑150 (MQ=255)

                                                                                                                                  ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCACCCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    <  2:4965035/150‑1 (MQ=255)

                                                                                                                                  ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    >  2:2649651/1‑150 (MQ=255)

                                                                                                                                  ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    <  1:6056792/150‑1 (MQ=255)

                                                                                                                                  ccTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTgaga                    <  2:4208128/150‑1 (MQ=255)

                                                                                                                                   cTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGagag                   >  1:772841/1‑150 (MQ=255)

                                                                                                                                   cTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGagag                   <  1:5950483/150‑1 (MQ=255)

                                                                                                                                    ttGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGc                  >  1:2347284/1‑150 (MQ=255)

                                                                                                                                    ttGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGc                  >  1:5186086/1‑150 (MQ=255)

                                                                                                                                       aGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATc               <  1:846668/150‑1 (MQ=255)

                                                                                                                                        gCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATcc              >  1:1580919/1‑150 (MQ=255)

                                                                                                                                        gCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATcc              <  1:4933525/150‑1 (MQ=255)

                                                                                                                                          agagCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTg            <  2:1903959/150‑1 (MQ=255)

                                                                                                                                          agagCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTg            <  2:5348975/150‑1 (MQ=255)

                                                                                                                                           gcgCCACCTTGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTgg           >  2:3146834/3‑150 (MQ=255)

                                                                                                                                            agCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGa          >  2:1763803/1‑150 (MQ=255)

                                                                                                                                             gCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGaa         <  1:4214481/150‑1 (MQ=255)

                                                                                                                                              ccaccaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAAc        >  2:4149166/1‑150 (MQ=255)

                                                                                                                                                accaAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAAccc      >  2:3014886/1‑150 (MQ=255)

                                                                                                                                                  caAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCAg    >  2:4804720/1‑150 (MQ=255)

GACCAGCTTCGTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGAGCCGCCTGGCCGTCTGGATTGCGCCTCTGCTGGTCGTGCTGGTCGGACTGCCAGGTTGCACGTCCTTGAGCAGAGCCACCAAGGTGGCGCTGGACCACTCGTTACCAATCGGGCCGCCCAAGGACAACCCAACCCAAGTCGCGTTCAGCATCAACGTCAGCCCAACGCTCAACAGCAACCCGAACAGTGTCGACGTGGCGGCCATCCCTGAGAGCATCCTGGAACCCAGCC  >  NC_002947/4612260‑4612557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`