Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,680,332 G→T 9.1% intergenic (+36/+68) PP_4137 → / ← chrR outer membrane siderophore receptor/chromate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,680,3320GT 9.1% 484.1 / 2.6 202intergenic (+36/+68)PP_4137/chrRouter membrane siderophore receptor/chromate reductase
Reads supporting (aligned to +/- strand):  ref base G (53/112);  new base T (5/21);  total (64/138)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CGCTATGCAGCGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAG  >  NC_002947/4680183‑4680480
                                                                                                                                                        |                                                                                                                                                    
cgcTATGCAGCGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTg                                                                                                                                                      <  2:194580/150‑1 (MQ=255)
  cTATGCAGCGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGaa                                                                                                                                                    <  1:3701409/150‑1 (MQ=255)
       cAGCGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑TCCCCCGAATGCGGGA‑GTGGATTTGc                                                                                                                                               >  1:2737080/1‑150 (MQ=255)
          cGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGa                                                                                                                                            <  1:3952809/150‑1 (MQ=255)
           gACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGAc                                                                                                                                           <  1:909842/150‑1 (MQ=255)
           gACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGAc                                                                                                                                           >  2:5112288/1‑150 (MQ=255)
           gACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGG‑TT‑ACCCGCGCATGCGTCA‑ATGAATTTGCCGAc                                                                                                                                           >  2:5086964/1‑150 (MQ=255)
             ccGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGc                                                                                                                                         <  2:4817206/150‑1 (MQ=255)
             ccGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑AACCGCCAAAGCGTCA‑TTGAAATTTCCCAccc                                                                                                                                         >  2:3793092/1‑148 (MQ=255)
              cGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCa                                                                                                                                        <  1:6367274/150‑1 (MQ=255)
               gTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTTCCGGCGCaa                                                                                                                                       >  2:4932629/1‑149 (MQ=255)
               gTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCAt                                                                                                                                       <  1:6001027/150‑1 (MQ=255)
                   cGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTcgc                                                                                                                                   <  2:6265329/150‑1 (MQ=255)
                   cGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTcgc                                                                                                                                   <  2:1842073/150‑1 (MQ=255)
                    gCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTcgcg                                                                                                                                  <  2:1075272/150‑1 (MQ=255)
                    gCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTcgcg                                                                                                                                  <  1:1542125/150‑1 (MQ=255)
                     ccGGCCTCCAACGACGCCGGCCAGGACAATGCCCGGTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCGTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGACTTTGCCGACGCATTCGCgg                                                                                                                                 <  2:4631441/150‑1 (MQ=255)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCG‑GCAAATTCAATGACGCATTCGCgg                                                                                                                                 <  1:4843140/150‑1 (MQ=37)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCgg                                                                                                                                 <  2:3114805/150‑1 (MQ=255)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCgg                                                                                                                                 >  2:2816952/1‑150 (MQ=255)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCgg                                                                                                                                 <  2:2266519/150‑1 (MQ=255)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCgg                                                                                                                                 <  2:4821610/150‑1 (MQ=255)
                     ccGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCgg                                                                                                                                 <  2:5612238/150‑1 (MQ=255)
                          cTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGG‑TT‑AACCGCGACATCGTCA‑TTTTATTTTCCGACGCAATAGCgggggaa                                                                                                                            >  2:4096490/1‑146 (MQ=255)
                           tACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑TAACGCGAAATAGGCAGTTTAATTTG‑CGACCCAATAGCGGGTaaaa                                                                                                                           >  1:1111469/1‑149 (MQ=255)
                           tACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAAc                                                                                                                           <  2:5202341/150‑1 (MQ=255)
                             cAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑TAACGCCCCAGCGTCA‑TTGGATTTTGCGACGCCTTGGCGGGGTAAccc                                                                                                                         >  2:6319011/1‑150 (MQ=255)
                              aaCGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGG‑TT‑TACCGCGAATTCGTCA‑TTGAATTTTGCGGCGCCATGGCGGGTGAAcccc                                                                                                                        >  2:6502570/1‑149 (MQ=255)
                                     ccGGCCAGGACAATGCCCGTTCGACACCGGGCGATGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTCCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTTAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTac                                                                                                                 <  1:5655277/150‑1 (MQ=255)
                                           aGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGCCGGGTGGGCGCGGGT‑TT‑ACCCGCGAAGGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAggg                                                                                                           <  2:447438/150‑1 (MQ=255)
                                           aGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAggg                                                                                                           <  2:5522253/150‑1 (MQ=255)
                                                actGCCCGTTCGACACCGGGCGAAGGATTGGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGATTTTGCCGACGCATTCGCGGTTAAACCCGCTCCTACACAGGGGCGtt                                                                                                      <  2:5945696/148‑1 (MQ=255)
                                                aaTGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑TCCCGCGGATGCGTGC‑TTGAATTTTGCGACGCATTAGCGGGTAAACCCGCTACTACACAGGGGCGtt                                                                                                      >  2:6658291/1‑150 (MQ=255)
                                                     ccGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGcaca                                                                                                 <  2:1507388/150‑1 (MQ=255)
                                                       gTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCCCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAACACCGCTCCTACACAGGGGCGGTGCAccgc                                                                                               >  2:5640403/1‑150 (MQ=39)
                                                        ttCGACACCGGGCGACGGATTGGGCGTGTATGCCGGGGTTTCCTACCGCTTCCGCTGAGGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTGGCCGACGCATTCGCGGGAAAACCCGCTCCTACACAGGGGCGTTGCAcagca                                                                                              <  1:6813040/150‑1 (MQ=39)
                                                        ttCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCAcagca                                                                                              <  2:5559510/150‑1 (MQ=255)
                                                        ttCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCAcagca                                                                                              <  2:5437786/150‑1 (MQ=255)
                                                         tccacacCGGGCTAAGGATTTGGCGTGTATGCGGGGGTTTCCTACAGCTTCCGCTTACGGGTGGGGGCGGTT‑TT‑ACCCGCGAATGCGTAT‑TTTAATTTGCCGACGCATTCGCGGTAAAACCCGCTCCTACACAGGGGCGTTGCAcagcag                                                                                             <  1:6114304/147‑1 (MQ=37)
                                                             cacCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGGATTTTCCGACGCATTAGCGGGTAAAACCGCTCCAACACAGGGGCGTTGCACAGCAGGAtt                                                                                         >  2:6129991/1‑150 (MQ=39)
                                                              acCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAAAGCGTCA‑TTGAAATTTCCGACGCATTAGCGGGTAAAACCGCACCTAAACAGGGGCGGTGCACAGCAGGATTa                                                                                        >  2:2197337/1‑150 (MQ=255)
                                                               ccGGGCGACGGTTTTGGCGTGTATGCCGGGTTTTCCTACAGCTTCCGCTGAGGGGTGGGCGCGGGT‑TT‑ACCCGCTAATGCGTAA‑TTGAATTTGCCGACGCATTCGGGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAg                                                                                       <  1:1012901/150‑1 (MQ=255)
                                                                cGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGAGGGGTGGGCGGGGTT‑TT‑ACCCGCGAATGCGTCT‑TTGAATTTGCCGACGAATTCGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAgg                                                                                      <  1:3359398/150‑1 (MQ=255)
                                                                 gggCGAAGGATTGGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TG‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAggg                                                                                     <  2:5038428/150‑1 (MQ=255)
                                                                  ggCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGG‑TG‑TCCCGCGAATGCGTCA‑TTGTCTTTGCCGACGCATTCGCGGGGAAACCCGCTCCGACACAGGGGCGGGGCACCGCGGGGTTAgggg                                                                                    >  2:1093970/1‑150 (MQ=255)
                                                                         gATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGAc                                                                             <  2:1119964/150‑1 (MQ=255)
                                                                             tGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGcc                                                                         <  2:4421176/150‑1 (MQ=255)
                                                                               gCGTGTCGGCCGGGGTTTCCTACAGCTTCCGCTGAGGGGGGGGCGCGGGT‑TT‑ACCCGCTACTGCGTAA‑TTGAATTTGCCGACGCATACGGGGGAAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCt                                                                       <  1:5399111/150‑1 (MQ=255)
                                                                               gCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑AACCGCGAATGGGTCA‑TTGAAATTTCCGGCGCATTCGCGGGTTAACCCCCGCCGACACCGGGGGGGTGCACCGCAGGCTTTGGGGTTCAGACCGcccg                                                                       >  2:5879723/1‑149 (MQ=255)
                                                                                 gtttctGCGGGGGTTTCCTACCGCTTCCGCTGCGGGGGGGGCGCGGTT‑TT‑ACCCGCTATTGCGAAA‑TTTATTTGGCCGCCGCTTGGGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGt                                                                     <  1:1813995/145‑1 (MQ=255)
                                                                                 gtgtATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCG‑GCAAATTCAATGACGCATTCGCGGGt                                                                                                                               <  1:5568077/92‑1 (MQ=17)
                                                                                   gttggggggggTTTCCTCCTGCTTCCGGTG‑CGGGGGGGGGGGGGT‑TTAACCGGCGATTGCGTCT‑TTTATTTGGCCGCCGCTTTGGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTc                                                                   <  1:5237141/143‑1 (MQ=255)
                                                                                    tATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCa                                                                  <  2:3365592/150‑1 (MQ=255)
                                                                                     aTGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCaa                                                                 <  2:5364892/150‑1 (MQ=255)
                                                                                     aTGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCaa                                                                 <  2:5813010/150‑1 (MQ=255)
                                                                                           gggTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGTGTT‑ACCC‑CCAAAGCGTCC‑TTTAAATTGCCGGCGCATTCGCGGGGAAAACCCCGGCGACACCCGGGGGGTGCACACCCGGTTTAGGGGTTCACCCCCCCCTGGTGAACATCAc                                                           >  2:4323877/1‑150 (MQ=255)
                                                                                                tCCTACAGCTTCCGCTGCGGGGGGGGCGGGGTT‑TT‑CCCCGCGAAGGCGTAA‑TTTAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATg                                                      <  1:4149189/150‑1 (MQ=38)
                                                                                                tCCTACAGCTGCCGCTGACGGGGGGGCGCGGGT‑TT‑ACCCGCGACTGCGTCA‑TTGAATTTGCCGACGCATTCGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATg                                                      <  2:3650512/150‑1 (MQ=255)
                                                                                                 ccTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGa                                                     <  1:5031639/150‑1 (MQ=255)
                                                                                                 ccTACAGCTCCCGGTGCGGGGTGGGGGCGGTT‑TT‑ACCCTCTATTGGTTAA‑TTTATTTTGCCCACGATTTGGGGGTTAAACCCGCTCCTACACAGGGGCGTTACACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGa                                                     <  1:2476652/150‑1 (MQ=255)
                                                                                                  cTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑AACCGCCAATGCGTCA‑TTGAATTTGCCGACGCATTAGCGGGTAAAACCGCTCCTACACAGGGGCGTTGGACAGCAGGATTAGGGGGTCAGAACGCCCTGTTCAACTACACCCAAGAc                                                    >  2:6792190/1‑150 (MQ=255)
                                                                                                   tACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACg                                                   <  2:709696/150‑1 (MQ=255)
                                                                                                       gCTTCCGCTGAGGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCT‑TTGAATTTGCCGACGCTTTCGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCaaa                                               <  1:3237153/150‑1 (MQ=255)
                                                                                                            cgcgGAGGGGTGGGGGCGGTT‑TT‑ACCCGCGAATGCTTCT‑TTTATTTTGCCGACGATTTGGGGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGt                                          <  1:3995505/150‑1 (MQ=255)
                                                                                                            cGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGt                                          <  1:6731764/150‑1 (MQ=255)
                                                                                                             ggtgcggggTGGGGGGGGTT‑TT‑ACCCGCGATTGCGTCT‑TTGATTTTGCCGACGCTTTCGGGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTc                                         <  1:3134423/144‑1 (MQ=255)
                                                                                                              cTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAAATCGTCA‑TTGAAATTTCCGGAGCATTAGCGGGTAAACCCCCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCATAACCCCCGGTTAAACATGAACCATGACGCACAACAGTCg                                        >  2:3908272/1‑150 (MQ=255)
                                                                                                                        ggCGCGGTT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCt                              <  2:776123/150‑1 (MQ=255)
                                                                                                                          cgcgGGT‑TT‑ACCCGCGAATGCGTCT‑TTTAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGc                            <  1:6807426/150‑1 (MQ=255)
                                                                                                                            gggtt‑tt‑ACCGGCTAATGCGACA‑TTTATTTTGCCGACGAATTGGCGGTAAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCaa                          <  1:1907729/146‑1 (MQ=255)
                                                                                                                             gggT‑TT‑ACCCGCGAATGCGTCA‑TTGATTTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAg                         <  2:5182074/150‑1 (MQ=255)
                                                                                                                               gT‑TT‑ACCCGCGAATGCGTCG‑GCAAATTCAATGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                       >  1:2222396/1‑150 (MQ=37)
                                                                                                                               gT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGaa                       <  2:2507707/150‑1 (MQ=255)
                                                                                                                                t‑tt‑ACCCGCGAATGCGTCA‑TTTAATTTGCCGACGCATTCGGGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAAc                      <  1:4782311/150‑1 (MQ=255)
                                                                                                                                  tt‑ACCCGCTAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACg                     <  2:5564939/150‑1 (MQ=255)
                                                                                                                                  tt‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACg                     <  2:5667351/150‑1 (MQ=255)
                                                                                                                                     aCCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGt                   >  1:3832269/1‑150 (MQ=255)
                                                                                                                                     aCCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGt                   >  2:1714900/1‑150 (MQ=255)
                                                                                                                                      cccGGGATTGCGTCA‑TTGAATTTGCCGACGCTTTCGGGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                  <  1:5415452/150‑1 (MQ=255)
                                                                                                                                      cccGCGAATGCGTCG‑GCAAATTCAATGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                  >  1:4872495/1‑150 (MQ=37)
                                                                                                                                      cccGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGTTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                  <  1:4405331/150‑1 (MQ=255)
                                                                                                                                      cccGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTc                  >  1:3224236/1‑150 (MQ=255)
                                                                                                                                       ccGCGAATGCGTCG‑GCAAATTCAATGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcg                 >  2:6413572/1‑150 (MQ=37)
                                                                                                                                       ccGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcg                 >  2:4399593/1‑150 (MQ=255)
                                                                                                                                        cgcgAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTcgc                <  2:3217886/150‑1 (MQ=255)
                                                                                                                                           gAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTc             >  1:654810/1‑150 (MQ=255)
                                                                                                                                            aaTGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCt            >  2:4402291/1‑150 (MQ=255)
                                                                                                                                             aTGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCtt           <  2:3576403/150‑1 (MQ=255)
                                                                                                                                             aTGCGACA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCtt           <  2:1272120/150‑1 (MQ=255)
                                                                                                                                              tGCGTCA‑TGGAATGTGCCGACGCATTCGGGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTg          <  1:6177879/150‑1 (MQ=255)
                                                                                                                                               gCGTCT‑TTGAATTTGCCGACTCTTTCGGGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGt         <  1:4932629/150‑1 (MQ=255)
                                                                                                                                                 gTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCa       >  1:4571160/1‑150 (MQ=255)
                                                                                                                                                 gTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCa       >  2:4173174/1‑150 (MQ=255)
                                                                                                                                                   cA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt     <  2:5610775/150‑1 (MQ=255)
                                                                                                                                                    ctttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAtt     <  1:3507625/148‑1 (MQ=255)
                                                                                                                                                    a‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTg    >  1:4326466/1‑150 (MQ=255)
                                                                                                                                                    a‑TTGAAGTTGCCGCCGCATTCGGGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTg    <  1:3793092/150‑1 (MQ=255)
                                                                                                                                                      ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGa   <  2:3248450/150‑1 (MQ=255)
                                                                                                                                                      ttGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCCGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGa   <  1:1663480/150‑1 (MQ=255)
                                                                                                                                                       tGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAg  >  1:5333585/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
CGCTATGCAGCGACCGTGACGCCGGCCTACAACGACGCCGGCCAGGACAATGCCCGTTCGACACCGGGCGAAGGATTTGGCGTGTATGCCGGGGTTTCCTACAGCTTCCGCTGACGGGTGGGCGCGGGT‑TT‑ACCCGCGAATGCGTCA‑TTGAATTTGCCGACGCATTCGCGGGTAAACCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAG  >  NC_002947/4680183‑4680480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑