Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,857,453	A→T	10.7%	intergenic (‑130/+167)	recR ← / ← ybaB	recombination protein RecR/nucleoid‑associated broad specificity regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,857,453	0	A	T	10.7%	152.7 / 7.1	54	intergenic (‑130/+167)	recR/ybaB	recombination protein RecR/nucleoid‑associated broad specificity regulator
Reads supporting (aligned to +/- strand):  ref base A (26/22);  new base T (2/4);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGTTT  >  NC_002947/4857305‑4857602                                                                                                                                                      |                                                                                                                                                      tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:1269297/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:5953701/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:1790495/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:1790551/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:1928781/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  2:3767196/150‑1 (MQ=255) tGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGaa                                                                                                                                                      <  1:5991058/150‑1 (MQ=255)  gagaGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAag                                                                                                                                                     <  2:2360093/150‑1 (MQ=255)    gagGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagca                                                                                                                                                   <  2:3771091/150‑1 (MQ=255)    gagGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagca                                                                                                                                                   <  2:3104641/150‑1 (MQ=255)      gggCTGAAGCTCTTGGGATAGGCCTGACAATTCAATCAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagcagc                                                                                                                                                 <  2:6022284/150‑1 (MQ=255)         cTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGcccc                                                                                                                                              <  1:2797248/150‑1 (MQ=255)         cTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGcccc                                                                                                                                              <  1:421970/150‑1 (MQ=255)         cTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTAGACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGcccc                                                                                                                                              <  2:3531090/150‑1 (MQ=255)          tGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCCGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCg                                                                                                                                             <  2:3850594/150‑1 (MQ=255)            aaGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttc                                                                                                                                           <  1:3093070/150‑5 (MQ=255)              gCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttcgc                                                                                                                                         <  1:5260507/150‑7 (MQ=39)              gCTCACGGGAAAGCCCTGACAATACACTAAGAGGCGGTTTATACCCACCGGCGGCGCTGGCGTCAAATCACCACGCCCAAA‑GCCTGCAAGGACTGCTTTATTGTGGGAGCGGCCTTCTGTCGCGAAAGGGCTGCGTCGCAGccctttcgc                                                                                                                                         <  2:1897654/150‑7 (MQ=255)                    gggAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttc                                                                                                                                           >  1:3671732/1‑138 (MQ=255)                    gggAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttc                                                                                                                                           <  2:3671732/142‑5 (MQ=255)                                       aaTACGGGGCGGTTCACCCCCGGGGGGGGGGGGGGCG‑CAAAACCCCCCCCCCAAACGCCGGCTCGGTCTTTTTTTTTGGGGGCGCGGCGTTGTGTGGCCAAGGGGCTGCCACGCCGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCg                                                                                                                <  2:167076/150‑1 (MQ=255)                                       aaTACGCGGGGGTTAATCCCCCGGGGGGGGGGTGGCGAAAACCCACCGCGCCCACC‑GCCTGCAGGGTCTGTTTTTTTGGGGGAGGGGCGTTGGGTCGCGAAGGGGCTGCGACGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCg                                                                                                                <  2:1496588/150‑1 (MQ=255)                                       aaTAAGCGGCGGTTTATCCCCTGGGGGGGGGGTGGGGTCAAATCACCTCGCCCACC‑GCCTGCAAGTTCTGCTTTTTTGTGGGTGCGGCGTTGTGTGGCGAAAGGGCTGCTAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCg                                                                                                                <  2:6185800/150‑1 (MQ=255)                                        aTAAGAGGCGTTTTATACCCAGGGGGGGCGGTGGCGTCAAAACACCTCGCCAAAA‑GCCTGAAAGTTATGATTTTTTGTGGGAGCGGCCTTGTGTCGCTAATGGGCTGCTAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCgg                                                                                                               <  2:3374180/150‑1 (MQ=37)                                        aTAAGAGGCGGTTTTTACCCCGGGGGGGGGGTGGCGTAAAATCACCTCGCCCAAC‑GCCTGAATGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCgg                                                                                                               <  2:5569405/150‑1 (MQ=37)                                         tatggggCGGTTTAGCCCCTGGGGGGGCGGGGGCTGCAAATCACCCCCCCCCCC‑GCCGCCACGGGCTGCTTTTTTGGGGGGGCGGCCTTGTGTGGCGAATGGGCGGCGACGCCGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCggg                                                                                                              <  2:6386052/145‑1 (MQ=255)                                         tAAGCGGGGGTTTATACCCTGGGGGGGGGGTGGCGTAAAAACACCTCGCCCAAA‑GCCTGAAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAATGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCggg                                                                                                              <  2:2200737/150‑1 (MQ=37)                                                 ggTTTATCCCCCGGGGGGGCGGTGGCCCAAAACCCCCCCGCCCACA‑GCCTGCAAGGTCGGTTTTTTGGTGGGCGCGGCTTTTTGTGGCTAACGGGCTGCGACCCCCCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTc                                                                                                      <  1:4965919/150‑1 (MQ=255)                                                 ggTTTACCCCCCGGGGGGGGGGGGGCGAAAACACCCCCCCCCCACC‑GCCTCCACGGTGTGATTTTTTGTGGGAGGGGCCTTGTGGCGCGAAGGGGCGGCGACCCCCCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTc                                                                                                      <  2:1384251/150‑1 (MQ=255)                                                   ttaacaCCCCGGGGGGGGGGGGGCGTAAAAACCCCGCCCCCAAC‑GCCTGCATGGTCTGATTTTTTGGGGGAGCGGCGTGGTGTCGCCAATGGGGGGCGAAGCCGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTcgc                                                                                                    <  2:971161/145‑1 (MQ=255)                                                            cgggggggcgggggcGAAAAACCACCTCGCCCAAC‑GCCTGAACGGTCTGTTTTTTTGTGGGAGGGGCGTTTTGTCGCGAATGGGCTGCTACGCTGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTc                                                                                           <  2:6626911/147‑1 (MQ=255)                                                                       gggCTTCAAAACCCCGCGCCAAAC‑GCCTGAATGGTGTGATTTTTTGTGGGTGCGGCGTTGTGTCGCGACCGGGCTGCGACGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAgg                                                                                <  2:4832627/149‑1 (MQ=21)                                                                                           cAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGccc                                                                                                                                               <  2:1117394/67‑1 (MQ=255)                                                                                           cAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGccc                                                                                                                                               >  1:1117394/1‑67 (MQ=255)                                                                                                                                cTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCaaaaaaaaacc                        >  1:5630203/1‑147 (MQ=255)                                                                                                                                    tgtCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCAc                    >  2:6338430/1‑150 (MQ=255)                                                                                                                                     gtCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACt                   >  1:5799167/1‑150 (MQ=255)                                                                                                                                        gcgAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgtt                >  1:1723632/1‑150 (MQ=255)                                                                                                                                        gcgAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgtt                >  1:1723857/1‑150 (MQ=255)                                                                                                                                        gcgAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgtt                >  1:2327516/1‑150 (MQ=255)                                                                                                                                          gAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACACGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttgt              >  2:4599631/1‑150 (MQ=255)                                                                                                                                          gAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttgt              >  2:3439195/1‑150 (MQ=255)                                                                                                                                          gAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttgt              >  2:4599771/1‑150 (MQ=255)                                                                                                                                             ggggCTGCTTCGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAg           >  2:473927/2‑150 (MQ=255)                                                                                                                                                gCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcgg        >  1:474663/1‑150 (MQ=255)                                                                                                                                                gCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcgg        >  1:2974152/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCa                     >  1:2732021/1‑135 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCa                     <  2:2732021/135‑1 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  2:392675/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  1:619761/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  2:6267632/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  1:4654132/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  2:362406/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGGTGTCAgcggcg      >  2:4877633/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTGTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg      >  2:1005983/1‑150 (MQ=255)                                                                                                                                                  tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAAACCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTGGGCAgcggcg      >  2:3613485/1‑150 (MQ=255)                                                                                                                                                    cGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGt    >  2:1569347/1‑150 (MQ=255)                                                                                                                                                    cGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGt    >  2:1569270/1‑150 (MQ=255)                                                                                                                                                     gAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGtt   >  1:6618018/1‑150 (MQ=255)                                                                                                                                                     gAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGtt   >  2:3644164/1‑150 (MQ=255)                                                                                                                                                     gAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGtt   >  2:3946904/1‑150 (MQ=255)                                                                                                                                                      aaGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGtgt  >  2:5755811/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                      TGAGAGGGCTGAAGCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAA‑GCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCGGTTT  >  NC_002947/4857305‑4857602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑