BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,889,957	C→A	5.5%	E139* (GAA→TAA) ‡	yedI ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,889,957	0	C	A	5.5%	1082.5 / 34.7	319	E139* (GAA→TAA) ‡	yedI	inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (174/127); new base A (9/9); total (183/136)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

CACACCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATCAACAACG  >  NC_002947/4889808‑4890106

 acacCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCa                                                                                                                                                      >  1:3541099/1‑150 (MQ=255)

   acCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaa                                                                                                                                                    >  2:2327062/1‑150 (MQ=255)

   acCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaa                                                                                                                                                    >  1:5111339/1‑150 (MQ=255)

   acCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaa                                                                                                                                                    <  1:2694008/150‑1 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACTATGATCTGCTGGGTCAGCGGCGAGTCAGCAACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaaa                                                                                                                                                   <  2:6791448/150‑1 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCTTTGATCGTGGCTGTGTCaaag                                                                                                                                                   >  2:6070116/1‑149 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaaa                                                                                                                                                   <  2:3547755/150‑1 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaaa                                                                                                                                                   <  2:5177083/150‑1 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaaa                                                                                                                                                   <  1:5293974/150‑1 (MQ=255)

    ccAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCaaaa                                                                                                                                                   <  1:2596176/150‑1 (MQ=255)

     cAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAg                                                                                                                                                  >  2:6857097/1‑150 (MQ=255)

     cAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAg                                                                                                                                                  >  1:5085926/1‑150 (MQ=255)

      aaTGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGc                                                                                                                                                 >  2:2542170/1‑150 (MQ=255)

         ggTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTCGATCTTGGCTTTTGAAAAAGccaa                                                                                                                                              <  2:2120213/150‑2 (MQ=255)

           tcatcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGccacca                                                                                                                                            <  2:135225/150‑1 (MQ=255)

           tcatcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGccacca                                                                                                                                            >  1:2319479/1‑150 (MQ=255)

           tcatcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTGAAAAAGccaaga                                                                                                                                            <  1:5780070/150‑4 (MQ=255)

            catcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAg                                                                                                                                           <  2:6328139/150‑1 (MQ=255)

            catcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAg                                                                                                                                           >  2:6680822/1‑150 (MQ=255)

             atcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGccacca                                                                                                                                            >  2:3077267/1‑148 (MQ=255)

             atcaCCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGccacca                                                                                                                                            <  1:3077267/148‑1 (MQ=255)

               caccacCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTc                                                                                                                                        >  1:6439965/1‑150 (MQ=255)

                accaccGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCa                                                                                                                                       >  1:4010959/1‑150 (MQ=255)

                 ccaccGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCaa                                                                                                                                      >  2:1106847/1‑150 (MQ=255)

                     cGCAATGCCGGAAAGCACGATGATCTGCAGGGTCATCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACt                                                                                                                                  >  1:544660/1‑150 (MQ=255)

                      gCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTg                                                                                                                                 <  1:1879577/150‑1 (MQ=255)

                      gCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTg                                                                                                                                 <  1:6865098/150‑1 (MQ=255)

                             cGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCgg                                                                                                                          <  1:6621964/150‑1 (MQ=255)

                                  aGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACg                                                                                                                     <  1:1399790/150‑1 (MQ=255)

                                  aGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACg                                                                                                                     <  1:4846653/150‑1 (MQ=255)

                                    cACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGc                                                                                                                   >  1:3220655/1‑150 (MQ=255)

                                    cACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGc                                                                                                                   <  2:4005761/150‑1 (MQ=255)

                                    cACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGc                                                                                                                   >  2:4306919/1‑150 (MQ=255)

                                    cACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGc                                                                                                                   <  1:4612244/150‑1 (MQ=255)

                                     aCGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGcc                                                                                                                  <  1:4463522/150‑1 (MQ=255)

                                     aCGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGcc                                                                                                                  >  1:5618737/1‑150 (MQ=255)

                                     aCGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGcc                                                                                                                  >  1:2189533/1‑150 (MQ=255)

                                       gatgatCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGcctc                                                                                                                >  2:4428577/1‑150 (MQ=255)

                                           atCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTg                                                                                                            <  1:5708340/150‑1 (MQ=255)

                                              tgctgGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTgcgc                                                                                                         >  2:1577634/1‑150 (MQ=255)

                                                 tgGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGcc                                                                                                      <  2:3133942/150‑1 (MQ=255)

                                                   ggTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTc                                                                                                    >  2:4492430/1‑150 (MQ=255)

                                                    gTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCg                                                                                                   >  2:4933731/1‑150 (MQ=255)

                                                     tCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGt                                                                                                  <  2:1215075/150‑1 (MQ=255)

                                                       aGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGc                                                                                                >  1:3803665/1‑150 (MQ=255)

                                                         cggcgAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCtg                                                                                              >  2:4962567/1‑150 (MQ=255)

                                                         cggcgAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCtg                                                                                              >  1:4158361/1‑150 (MQ=255)

                                                          ggcgAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCtgt                                                                                             <  2:5880487/150‑1 (MQ=255)

                                                            cgAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGc                                                                                           <  2:2504609/150‑1 (MQ=255)

                                                                  aGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATc                                                                                     >  1:2474758/1‑150 (MQ=255)

                                                                        gATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTc                                                                               <  1:621716/150‑1 (MQ=255)

                                                                         aTACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTAGATCTTGGCTTTTTCAAACGCCACCAGGTCAACACTGGCATCGCCAACGGCCTCCTAGCGCGCCTCGGGCTGTGCCTCATCCACGTcc                                                                              >  2:4042425/1‑150 (MQ=255)

                                                                          tACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCt                                                                             <  1:3171799/150‑1 (MQ=255)

                                                                              cAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCt                                                                         >  2:1068325/1‑150 (MQ=255)

                                                                               aaGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCtg                                                                        >  2:2444959/1‑150 (MQ=255)

                                                                                  gTAATGGCGATGATTTCTGCCGACAGGATGAATTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGc                                                                     <  2:5085926/150‑1 (MQ=255)

                                                                                     aTGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAg                                                                  >  2:2063653/1‑150 (MQ=255)

                                                                                      tGGCGATGATTCCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGa                                                                 <  2:3743711/150‑1 (MQ=255)

                                                                                        gCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAAc                                                               <  1:6780585/150‑1 (MQ=255)

                                                                                          gatgatTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACtt                                                             >  1:783364/1‑150 (MQ=255)

                                                                                          gatgatTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACtt                                                             >  1:1366078/1‑150 (MQ=255)

                                                                                          gatgatTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACtt                                                             >  2:3392197/1‑150 (MQ=255)

                                                                                                ttCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTgcgc                                                       >  1:4663832/1‑150 (MQ=255)

                                                                                                ttCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTgcgc                                                       >  1:5823741/1‑150 (MQ=255)

                                                                                                 tCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCTCCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGcc                                                      <  2:624399/150‑1 (MQ=255)

                                                                                                   tGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAg                                                    >  1:5972753/1‑150 (MQ=255)

                                                                                                    gCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGc                                                   <  2:6127347/150‑1 (MQ=255)

                                                                                                     ccGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCt                                                  <  1:1109552/150‑1 (MQ=255)

                                                                                                            gATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGaa                                           >  2:6586530/1‑150 (MQ=255)

                                                                                                            gATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGaa                                           >  1:374918/1‑150 (MQ=255)

                                                                                                             aTGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGaaa                                          <  1:2225737/150‑1 (MQ=255)

                                                                                                                 aGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCt                                      <  1:4894475/150‑1 (MQ=255)

                                                                                                                  gTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTc                                     <  1:1559500/150‑1 (MQ=255)

                                                                                                                  gTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCACCAGCTTCTCGAAACCCTc                                     <  1:2930939/150‑1 (MQ=255)

                                                                                                                    cGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGa                                   >  2:195433/1‑150 (MQ=255)

                                                                                                                     ggTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGaa                                  >  2:1001103/1‑150 (MQ=255)

                                                                                                                     ggTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGaa                                  <  1:5194403/150‑1 (MQ=255)

                                                                                                                      gTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAg                                 <  1:4310038/150‑1 (MQ=255)

                                                                                                                       tACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGc                                <  2:2847639/150‑1 (MQ=255)

                                                                                                                        aCGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGca                               <  1:3564565/150‑1 (MQ=255)

                                                                                                                          gCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGACAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAAccc                                       >  2:3319564/1‑140 (MQ=255)

                                                                                                                          gCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGACAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAAccc                                       <  1:3319564/140‑1 (MQ=255)

                                                                                                                           cACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAg                            <  2:1810314/150‑1 (MQ=255)

                                                                                                                              cGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGaa                         >  2:5353010/1‑150 (MQ=255)

                                                                                                                              cGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGaa                         <  1:4978961/150‑1 (MQ=255)

                                                                                                                               gCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAAc                        <  2:4245813/150‑1 (MQ=255)

                                                                                                                                 ccccTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAAcgc                      <  1:1003872/150‑1 (MQ=255)

                                                                                                                                    cTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGcc                   >  1:2454844/1‑150 (MQ=255)

                                                                                                                                      tGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCAc                 <  1:6148781/150‑1 (MQ=255)

                                                                                                                                         tggtGGCTTTTGAAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCa              >  2:3951094/4‑150 (MQ=255)

                                                                                                                                          ggtGGCTTTTGAAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAg             >  1:1642699/3‑150 (MQ=255)

                                                                                                                                           ttGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGc            >  2:27331/1‑150 (MQ=255)

                                                                                                                                             ggCTTTTGAAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCAt          <  2:3880139/150‑1 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       >  2:4757386/1‑150 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       <  2:464194/150‑1 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       >  1:6719690/1‑150 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       <  2:4158361/150‑1 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       >  2:3644182/1‑150 (MQ=255)

                                                                                                                                                tttttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaa       >  2:3052004/1‑150 (MQ=255)

                                                                                                                                                  tttCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATcaaca     >  2:348283/1‑150 (MQ=255)

CACACCAATGGTCATCACCACCGCAATGCCGGAAAGCACGATGATCTGCTGGGTCAGCGGCGAGTCAGCCACGATACCCAAGGTAATGGCGATGATTTCTGCCGACAGGATGAAGTCGGTACGCACCGCCCCCTTGATCTTGGCTTTTTCAAAAGCCACCAGGTCAACACTGGCATCGGCAACGGCCTCCTTGCGCGCCTCGTGCTGTGCCTCATCCTCTTCCTTGCTGTGCAAGAACTTGTGCGCCAGCTTCTCGAAACCCTCGAAGCACAGGAACGCGCCACCCAGCATCAACAACG  >  NC_002947/4889808‑4890106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`