BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,084,426	G→T	12.6%	intergenic (‑51/+24)	PP_t55 ← / ← PP_t54	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,084,426	0	G	T	12.6%	497.4 / 48.8	181	intergenic (‑51/+24)	PP_t55/PP_t54	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (94/62); new base T (8/16); total (103/78)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

AGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGC  >  NC_002947/5084278‑5084572

aGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGc                                                                                                                                                   <  2:495027/150‑1 (MQ=255)

  cagttccACTTCAAGATAGTGTCTGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCaa                                                                                                                                                 <  1:4670960/145‑1 (MQ=255)

  cAGAACCACTTCAAGCCAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCaa                                                                                                                                                 <  2:2363097/150‑1 (MQ=255)

  cAGAACCACTTCAAGATAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCaa                                                                                                                                                 <  1:4886205/150‑1 (MQ=255)

   aGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGGTTGACTGAAATGGCTTTGCAAg                                                                                                                                                >  2:6621229/1‑150 (MQ=255)

    gAACCACTTCACGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGc                                                                                                                                               <  2:2006323/150‑1 (MQ=255)

    gAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGTCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACGGGTGGTGGATTGAGATGGCTTTGCAAGc                                                                                                                                               >  2:2652119/1‑150 (MQ=14)

    gAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTATACCAGATTACGGATGGTTCACTGAAAAGGCTTTGaaacc                                                                                                                                               >  2:4451671/1‑148 (MQ=14)

    gAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAAACTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGc                                                                                                                                               <  2:4268526/150‑1 (MQ=255)

     caCCACTTCAAGACAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCa                                                                                                                                              <  1:1501055/149‑1 (MQ=255)

     aaCCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTGAAACCAGATTACCGCGGGGTGACTGAAATGGCTTTGCTAGCa                                                                                                                                              >  2:2151075/1‑150 (MQ=255)

        cACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGTTTGAATGAAATGGCTTTGAAAgaagaa                                                                                                                                           >  2:5159004/1‑150 (MQ=14)

           ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTATCCGAGTACGCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGATATGGCTTTGCAAGCAGAACCa                                                                                                                                        >  1:6294507/1‑150 (MQ=255)

           ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGACGAGTACTCTATCCAGCTGAGCTATGAGTCCGCTATGGGCAGTTTTATACCAGATAACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGACCCa                                                                                                                                        >  1:6005361/1‑150 (MQ=17)

             cAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGGTTGACTGAAAAGGCTTTGCAAGCAGAACCACt                                                                                                                                      >  2:1330974/1‑150 (MQ=255)

                gattgTGGCTGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCa                                                                                                                                   <  1:4645904/146‑1 (MQ=255)

                     tGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGaaa                                                                                                                              <  1:1813596/150‑1 (MQ=255)

                        cGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTg                                                                                                                           <  1:2601525/150‑1 (MQ=255)

                        cGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTg                                                                                                                           <  2:5478978/150‑1 (MQ=255)

                         ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAg                                                                                                                                 >  2:3922442/1‑143 (MQ=255)

                          gACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGc                                                                                                                         <  1:6311952/150‑1 (MQ=255)

                          gACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAGGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGc                                                                                                                         <  2:5709739/150‑1 (MQ=255)

                             tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTGCTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                                                                      <  2:4123447/150‑1 (MQ=255)

                             tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTCCCGCGGGTGGACTGAAATGGCTTTGCAAGCAGCCCCACTGCAAGAAAGTGGCggc                                                                                                                      >  2:1357844/1‑149 (MQ=17)

                                 cagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                                                                  <  1:3009122/150‑1 (MQ=255)

                                    gATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGTTTGACTGAAATGGCTTTGCAAGCAGAACCACATCAAGAAAGTGGCGGACTcagca                                                                                                               >  2:6824851/1‑150 (MQ=255)

                                    gATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagca                                                                                                               <  1:5036977/150‑1 (MQ=255)

                                       tCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGa                                                                                                            <  1:1202218/150‑1 (MQ=255)

                                        cGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGAt                                                                                                           <  1:1881137/150‑1 (MQ=255)

                                         gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGCTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGAtt                                                                                                          >  2:5039932/1‑150 (MQ=255)

                                         gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCGGGTTGACTGACATGGCTTTGCAAGCAGACCCACTGCAAGAAAGAGGCGGACTCAGCAggcat                                                                                                          >  2:4027095/1‑147 (MQ=17)

                                          tcccTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc                                                                                                         <  1:1907961/148‑1 (MQ=255)

                                              tGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTTAACACGATTACCGCTGGTTGAGTGAAATGGATTTGCAACCAGAACAACTCCAAGAACTTGGCGGACACAGCAGGATTCGaga                                                                                                     >  2:1907961/1‑148 (MQ=17)

                                              tGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAAc                                                                                                     <  2:2341522/150‑1 (MQ=255)

                                                 gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTACACCAGATTACCTCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCta                                                                                                  <  2:2794674/150‑2 (MQ=255)

                                                 gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGGTGGTTTACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGTATTCGAACCTg                                                                                                  >  2:2403792/1‑150 (MQ=255)

                                                     gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGTCAGTTTTAAACCAGATTACCGCTGGTTGACTGATATGGCTTTGCAATCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGAc                                                                                              <  1:3975583/150‑1 (MQ=255)

                                                     gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGAGTGGCAGTTTTAAACCAGATACCCGCGGGTTGACTGAACGGGCGATCCAAGCCGAACCACTGCCAGAACGTGGCGGACTCAGCCGGAGTCGAACCGGCGAc                                                                                              >  2:6375370/1‑150 (MQ=17)

                                                          gctCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATTGATTTTCTTGCAGAAACACGTGATGTATGTGGCTGACTCAGCAGGATTCGAACCTGCGACCGCTc                                                                                         <  1:4246490/148‑1 (MQ=17)

                                                          gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTc                                                                                         >  2:4372125/1‑150 (MQ=255)

                                                          gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTc                                                                                         >  2:939661/1‑150 (MQ=255)

                                                            tCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGGTTGACTGAAATGGCTTTGCAAGCACTACCACTTCAAGAAAGTGGCGGACTCAGAAGGAATCGAACCTGCGACCGCTCgg                                                                                       >  2:4844593/1‑150 (MQ=17)

                                                                   cGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAg                                                                                >  2:2035459/1‑150 (MQ=255)

                                                                         ctctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt                                                                          >  1:4895019/1‑150 (MQ=255)

                                                                         ctctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCGGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt                                                                          >  1:3711783/1‑150 (MQ=255)

                                                                          tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                                                                         >  1:3082879/1‑150 (MQ=255)

                                                                            tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                                                                       >  1:3826573/1‑150 (MQ=255)

                                                                              tCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTActct                                                                     >  2:6112456/1‑150 (MQ=255)

                                                                                 aGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                  >  1:4514777/1‑150 (MQ=255)

                                                                                    tGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                               >  1:6090432/1‑150 (MQ=255)

                                                                                      aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                             >  2:5883816/1‑150 (MQ=255)

                                                                                        cTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGATCGTAGCCGAGTACTCTCTCCAGCTGa                                                           >  2:6248147/1‑150 (MQ=255)

                                                                                         tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                          >  1:1098807/1‑150 (MQ=255)

                                                                                          aTGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                         <  2:2018981/150‑1 (MQ=255)

                                                                                           tGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                        >  1:6439858/1‑150 (MQ=255)

                                                                                           tGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGCTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                        <  1:5039932/150‑1 (MQ=255)

                                                                                             actacgcgCTTGGCAGTTTTACACCAGCTTACCTCTGGGTGACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                                                      <  2:2221884/146‑1 (MQ=11)

                                                                                             aGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                                                      <  1:1704/150‑1 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    <  1:6568912/150‑1 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    >  2:1701479/1‑150 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    >  1:4183952/1‑150 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    >  1:4896319/1‑150 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    >  2:2871833/1‑150 (MQ=255)

                                                                                               tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa                                                    >  1:5975603/1‑150 (MQ=255)

                                                                                                ccGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg                                                   >  1:1257835/1‑150 (MQ=255)

                                                                                                  gcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTc                                                 >  1:4538781/1‑150 (MQ=255)

                                                                                                          cAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGAAAATg                                         >  2:5194182/1‑150 (MQ=255)

                                                                                                           aGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTgg                                        >  1:2957986/1‑150 (MQ=255)

                                                                                                            gTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGTGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGc                                       <  2:6206946/150‑1 (MQ=255)

                                                                                                                      aGATTAGCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                             >  1:4735661/1‑150 (MQ=255)

                                                                                                                      aGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGAAAGTGGCAGATATAGAc                             >  2:3149938/1‑150 (MQ=255)

                                                                                                                             cGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATAc                      >  2:1862217/1‑150 (MQ=255)

                                                                                                                             cGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATAc                      >  2:5325192/1‑150 (MQ=255)

                                                                                                                                  gatgtcTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTg                 <  1:137243/145‑1 (MQ=255)

                                                                                                                                    ttaCTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGa                         <  2:1488779/138‑1 (MQ=11)

                                                                                                                                     taCTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGtt              <  2:1706036/149‑1 (MQ=25)

                                                                                                                                     taCTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGtt              <  1:5652503/149‑1 (MQ=25)

                                                                                                                                     taCTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGtt              <  2:1394255/149‑1 (MQ=40)

                                                                                                                                       cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttga            <  1:943700/150‑1 (MQ=40)

                                                                                                                                       cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttga            <  2:4514777/150‑1 (MQ=40)

                                                                                                                                       cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttga            <  1:6820031/150‑1 (MQ=40)

                                                                                                                                       cTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttga            >  2:5947986/1‑150 (MQ=40)

                                                                                                                                        tGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAGACCAGATACCGCTGGttga            <  1:5867678/149‑1 (MQ=14)

                                                                                                                                        tGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAGACCAGATACCGCTGGttga            >  2:5867678/1‑149 (MQ=14)

                                                                                                                                        tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat           >  2:3077605/1‑150 (MQ=255)

                                                                                                                                        tGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgat           >  2:1088850/1‑150 (MQ=255)

                                                                                                                                          aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                             >  1:532801/1‑130 (MQ=17)

                                                                                                                                          aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                             <  2:1161113/130‑1 (MQ=17)

                                                                                                                                          aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                             >  1:831135/1‑130 (MQ=17)

                                                                                                                                          aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAc                             <  2:831135/130‑1 (MQ=17)

                                                                                                                                          aaaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgattg         >  2:1822525/1‑150 (MQ=255)

                                                                                                                                          aaaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGATATAGACCAGATACCGCTCGttgagag         >  1:4975179/1‑147 (MQ=255)

                                                                                                                                           aaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGttgattga        <  1:5414298/150‑1 (MQ=38)

                                                                                                                                           aaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCAGGttgattga        >  1:6549532/1‑150 (MQ=255)

                                                                                                                                            aTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAg                               <  2:4985381/126‑1 (MQ=11)

                                                                                                                                            aTGGCTTTGCAAGCAGAACCTCTTCAAGTAAGTTGCGGTCTCTGCAGGATTCGAAGCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa       <  1:4844593/150‑1 (MQ=14)

                                                                                                                                               gCTTTGCAAGAAGTACCACATCAAGAAAGTGGCGTACTCAGCAGTATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAagca    <  1:4930838/150‑1 (MQ=14)

                                                                                                                                                cTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGaa       >  2:490078/1‑146 (MQ=38)

                                                                                                                                                 ttttCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGa                         >  1:1071332/1‑127 (MQ=17)

                                                                                                                                                 tttGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCCAGTGGCAGGGGTAGACCAGAAACCGCTGGTTGATTGAagcagc  >  1:2261771/1‑150 (MQ=14)

AGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACCGCTGGTTGATTGAAGCAGC  >  NC_002947/5084278‑5084572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`