BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,228	G→C	8.4%	intergenic (+222/+70)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,228	0	G	C	8.4%	451.2 / 26.2	143	intergenic (+222/+70)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (78/51); new base C (2/10); total (80/63)
Fisher's exact test for biased strand distribution p-value = 4.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 149 total aligned reads displayed.

TGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATA  >  NC_002947/5102079‑5102376

  cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGa                                                                                                                                                      >  2:5278987/1‑150 (MQ=255)

     aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                   <  1:1734015/150‑1 (MQ=255)

       aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAATGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGGCAGCt                                                                                                                                                 >  2:1704489/1‑150 (MQ=255)

         tGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                              <  2:4947621/150‑1 (MQ=255)

            tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                           <  1:6114791/150‑1 (MQ=255)

              aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                         <  1:5936365/150‑1 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACCGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTAGCCCGCGCATGCGGGAGGTCAGGGGCCAGCG‑TAGCCTGaa                                                                                                                                       >  2:6430789/1‑150 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTgta                                                                                                                                       >  2:3163541/1‑148 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTGAGGGGACAGCT‑TAGCCTGaa                                                                                                                                       >  2:3163627/1‑150 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                       <  2:5148412/150‑1 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                       <  2:2275505/150‑1 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                       >  2:760574/1‑146 (MQ=255)

                tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTCCGGGGCC‑GGTGACAGAACAGTAACCGATGACAGACGGGTAACAATGCAATTGCGGCCGTTCCCGCGAAAGCCTGAGGGCCCGGGACAGCT‑AAGccataa                                                                                                                                       >  2:4034613/1‑146 (MQ=255)

                  cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGGCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TGTCCGGAACt                                                                                                                                     >  1:2328048/1‑150 (MQ=255)

                     tGCACCGAAACGGGCCGGACTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                  <  2:5444305/150‑1 (MQ=255)

                     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                  <  1:1470676/150‑1 (MQ=255)

                     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                  <  1:5790312/150‑1 (MQ=255)

                     tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                  <  2:4340414/150‑1 (MQ=255)

                        aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCa                                                                                                                               <  2:3896620/150‑1 (MQ=255)

                        aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCa                                                                                                                               >  2:6778825/1‑150 (MQ=255)

                              aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                         <  1:6001794/150‑1 (MQ=21)

                              aCGGGCCGGAATTGGGTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                         <  2:4766572/150‑1 (MQ=21)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        <  2:4732994/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        <  1:6425583/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        <  2:6639158/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        <  2:3673350/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        >  2:3578442/1‑150 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                        <  1:3416542/150‑1 (MQ=255)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                        <  2:2122742/150‑1 (MQ=21)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                        <  1:2184440/150‑1 (MQ=21)

                               cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                        <  1:4371051/150‑1 (MQ=21)

                                gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                       >  2:5997961/1‑150 (MQ=255)

                                 ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGc                                                                                                                      <  1:5015328/150‑1 (MQ=21)

                                 ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGATGTAAGGGGCCAGCG‑TAGCCGGAACTGACGCAATAGCGGGc                                                                                                                      >  1:353528/1‑150 (MQ=255)

                                   ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                    <  2:2287833/150‑1 (MQ=255)

                                    cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGAAGCATTAGCGGGCAA‑g                                                                                                                  >  2:1444565/1‑150 (MQ=255)

                                      gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑Gcc                                                                                                                <  2:1870415/150‑1 (MQ=255)

                                            tttCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCAATTCGGGAGGTGAGGGGACAGCT‑TATCCGGAACGTACGCAATCGCGGGCAA‑GCCCGCGcc                                                                                                          >  1:330212/1‑150 (MQ=255)

                                             ttCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGACCGCCACTGCGGGAGGTCAGGGGGCAGCG‑TAGCCGGAACCGACGCAGTCGCGGgccc‑gcccccaccg                                                                                                         >  1:6488073/1‑144 (MQ=255)

                                               ccTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGc                                                                                                       >  2:5610052/1‑150 (MQ=255)

                                                 tGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAg                                                                                                     >  2:738482/1‑150 (MQ=255)

                                                     gcCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACGGACGCATTCGCGGGCCA‑GCCCGCTCCTGCAggggg                                                                                                 >  1:1125420/1‑150 (MQ=255)

                                                     gcCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑GAGCCGGAACTTAAGCAGTCGCGGGCAA‑GCCCCCACCAGCAggggg                                                                                                 >  1:3922413/1‑150 (MQ=255)

                                                     gcCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCCAAGCCTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCTGCAggggg                                                                                                 >  2:4197127/1‑150 (MQ=255)

                                                        ccGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGCAAGGGGGAGGTCCGGGGCCAGCG‑TAGGCTGAACAGAACCATTAGCGGGCAA‑GCCCGCGCCAGCCggggggcg                                                                                              >  1:621663/1‑147 (MQ=255)

                                                        ccGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGGGAGGTCAGGGGACCCCG‑TAGCCGGCACAGACGCAATCCCGGGCCA‑GCCCGCGCCAGCAGGGGGTAt                                                                                              >  1:6162449/1‑150 (MQ=255)

                                                             ccTGTGCGGGGCG‑GGTGACAGAACATTAACCGATGATTGTCGGTTAAAAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCg                                                                                         <  2:6229551/150‑1 (MQ=39)

                                                                 tgCGGGGCC‑GGTGACAGAACAGTACCCGATGTTTGGCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGAGGTCAGGGGACAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                     <  2:3952833/150‑1 (MQ=255)

                                                                 tgCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAAAGCGTGGGGTGAGGGGACAGCT‑TTGGCGGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCGGGCGGGGGGGTTGCCggggc                                                                                     >  1:4624211/1‑148 (MQ=255)

                                                                    ggggCCGGGTCACACAACTGTACCGGATGTTTGTCGTTTAAAACTGCAAACGGGGGCTTGCCGGCCATTGGGTGTGGTACGGGTACAGCT‑TCGCCTGAAGGGACGATTTGGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                   <  2:1503259/150‑1 (MQ=255)

                                                                    ggggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGGTTGCCGGGAAggg                                                                                  >  2:1893176/1‑150 (MQ=255)

                                                                    ggggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCGCATGCGGGCGGGCCGGGGGCCGCG‑TAGCCGGGACAGAAGCAGTCGCGGGCCA‑GCCCCCGCCGGCAGGGGGGATTGCCggggcggg                                                                                  >  1:3001811/1‑145 (MQ=255)

                                                                     gggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTGCCGCGAATGCGTGAGGGGCGGGGGCAGCG‑TAGGCGGGACTGACGCAGTCGCGGGGAA‑GCCCCCGCCGGCCGGGGGGGTTGCCGGGGAgggg                                                                                 >  1:3626055/1‑149 (MQ=255)

                                                                     ggccc‑GGTTAAAGAACCTTAACCTATTATTGTCGGTAAACAATGAAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTATGGGGACAGAT‑TACCCTGAACTGACGCATTCGCGGGAAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGt                                                                                 <  2:1612100/147‑1 (MQ=255)

                                                                      ggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAA‑GCCCGCTCCTTCAGGGGGGATTGCCGGGAAGGGtt                                                                                >  2:3064226/1‑150 (MQ=255)

                                                                         cgggTGACATAACATTAACCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGTGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                              <  2:2740430/148‑1 (MQ=255)

                                                                                 aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCCAATTCGTGAGGTCAGGGGGCACCT‑TATCCGGAACGGACGCAATAGCGGGCCA‑CCCCCCGCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAg                                                                      >  1:111352/1‑150 (MQ=255)

                                                                                 aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGACGGGTTTACGCCGAAg                                                                      >  1:5057228/1‑150 (MQ=255)

                                                                                 aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTCTTGCCGGGCAGGGTTTAAGCCGTAg                                                                      >  2:6500934/1‑150 (MQ=39)

                                                                                 aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCAGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTTCCGGGAAGGGTTTAAGCCGAAg                                                                      >  2:24955/1‑150 (MQ=255)

                                                                                     ctgTAACCGATGATGGTCGGTTAACAATGCAAATGCGGGCTGGCCCGCGATTGCGTGTGGCCTGGGGACAGCT‑TAGCCTGAACTGAGGCATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                  <  2:1057323/148‑1 (MQ=255)

                                                                                        tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                               >  2:5042795/1‑150 (MQ=255)

                                                                                        tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                               >  2:3837473/1‑150 (MQ=255)

                                                                                          aCCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGTGGTCTGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                             <  2:2805912/150‑1 (MQ=255)

                                                                                          aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                             >  2:6090232/1‑150 (MQ=255)

                                                                                             gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCg                                                          >  2:1484756/1‑150 (MQ=255)

                                                                                             gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGGGGGCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCAGGTTCaaag                                                          >  2:229852/1‑147 (MQ=255)

                                                                                                 atTGTCGGGTACCACCCCAAACGCGGGCTGGCCCGCTATTGGGTGCGGTCAGGGGACAGCT‑TCGCCTGAACTCCCCCATTCGGGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                      <  2:2639282/150‑1 (MQ=255)

                                                                                                    gtgggttAACAATGCAAACGGGGGCTTGCCCGCGAATGCGTGCGGTCTGGGGACAGCT‑TAGCCTCAACGGACGAATCCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgtg                                                   <  2:4347900/144‑1 (MQ=255)

                                                                                                    gTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGTCAGCT‑TAGCCTGAACGGTCGCGTTCGCGGGCAA‑GCCCGCTGCGGCAGGGGGTGTTGCCGGGCAGGGTTTACGCCGAAGCCAGGGTAATCGCCTtgtg                                                   >  2:5357049/1‑150 (MQ=255)

                                                                                                     tCGGTTAACATTGCAAACGCGGGCTTGCCCGCTAATGCGTTAGGTCTGGGTACAGAT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                  <  2:2119238/150‑1 (MQ=255)

                                                                                                     tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                  >  2:6791274/1‑150 (MQ=255)

                                                                                                        gttaaataTGCAAAAGGGGGTTTGCCCGGTTTTGCTTGTGTTCGGGTTAATGAT‑TCGCCTCAAATGCCGAATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                               <  1:5711540/143‑1 (MQ=255)

                                                                                                        ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                               >  2:2202833/1‑150 (MQ=255)

                                                                                                                  cAAAAGCGGGCTTGCCCCCGAATGCGTGATGTCAGGGGACAGCG‑TAGCCGGCAAATACGCATTAGCGGGCAA‑GCCCCCACCATCCGGGGGTATTTCCGGGAAGGGTTTACGCCGAACCCACGTTCATAGCCTTGTGGGTATCGATCATGt                                     >  2:5170096/1‑150 (MQ=255)

                                                                                                                   attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                    >  2:6105896/5‑150 (MQ=255)

                                                                                                                           gCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAA‑GCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                            >  2:1024597/1‑150 (MQ=18)

                                                                                                                             ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                          >  1:966987/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  2:5131677/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  1:2707981/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  2:4581113/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  1:4213730/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  1:5444030/1‑150 (MQ=255)

                                                                                                                                cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                       >  1:1980240/1‑150 (MQ=255)

                                                                                                                                 ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      >  1:5949125/1‑150 (MQ=255)

                                                                                                                                 ccGCGAAGCCGTGAGGTCAGGGGACCGCT‑CACCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                      <  2:5318345/150‑1 (MQ=255)

                                                                                                                                  cgcgAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                     <  2:6588364/150‑1 (MQ=255)

                                                                                                                                   gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                    >  1:1639150/1‑150 (MQ=255)

                                                                                                                                   gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                    >  1:3963743/1‑150 (MQ=255)

                                                                                                                                    gttttGCTTGTGGTCGGGGGCCTGCT‑TCGCCCCAAGTGCCGC‑TTCCGGGGCAACCCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                   <  1:1061555/146‑1 (MQ=255)

                                                                                                                                     gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                  >  1:2014613/1‑150 (MQ=255)

                                                                                                                                         gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                   >  1:550024/1‑81 (MQ=255)

                                                                                                                                                gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg       >  2:2404806/1‑150 (MQ=255)

                                                                                                                                                 tctggggACATCT‑TAGCCTTAACTGACGAATTCGCTGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCATGTGCTGTACCACACCCGTGTCAGCCAAGGtata      <  2:5054101/147‑4 (MQ=255)

                                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga      >  2:6100636/1‑150 (MQ=255)

                                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga      >  2:4841083/1‑150 (MQ=255)

                                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga      >  1:4518366/1‑150 (MQ=255)

                                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga      >  2:2965814/1‑150 (MQ=255)

                                                                                                                                                 tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCAAAGGTCga      >  2:4840930/1‑150 (MQ=255)

                                                                                                                                                     gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata  >  1:1727633/1‑150 (MQ=255)

TGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATA  >  NC_002947/5102079‑5102376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`