BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,237	A→C	13.8%	intergenic (+231/+61)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,237	0	A	C	13.8%	390.3 / 34.0	154	intergenic (+231/+61)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (86/42); new base C (2/21); total (91/63)
Fisher's exact test for biased strand distribution p-value = 1.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

TGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5102088‑5102386

   tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                     <  2:4914340/150‑1 (MQ=255)

   tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                     <  1:6114887/150‑1 (MQ=255)

     aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                   <  1:5936346/150‑1 (MQ=255)

     aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                   <  1:5936365/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTgta                                                                                                                                                 >  2:3163541/1‑148 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTGAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                 >  2:3163627/1‑150 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                 <  2:6264366/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                 <  2:5148412/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                 <  2:2275505/150‑1 (MQ=255)

       tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                                 >  2:760574/1‑146 (MQ=255)

            tGCACCGAAACGGGCCGGACTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  2:5444305/150‑1 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  2:244196/150‑1 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  1:1470676/150‑1 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  2:4340418/150‑1 (MQ=255)

            tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                            <  1:1470480/150‑1 (MQ=255)

               aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCa                                                                                                                                         >  2:6778825/1‑150 (MQ=255)

                    aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                                                                                                                                    <  1:2381229/150‑1 (MQ=21)

                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                   <  1:6001794/150‑1 (MQ=21)

                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                   <  1:3005047/150‑1 (MQ=21)

                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                   <  1:6001761/150‑1 (MQ=21)

                     aCGGGCCGGAATTGGGTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                   <  2:4766572/150‑1 (MQ=21)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  2:3673350/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  >  2:3578442/1‑150 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  1:6425583/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  2:6639158/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  1:3416542/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  2:4732994/150‑1 (MQ=255)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                  <  1:2184440/150‑1 (MQ=21)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                  <  2:2122742/150‑1 (MQ=21)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                  <  1:4371051/150‑1 (MQ=21)

                      cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                  <  1:1706720/150‑1 (MQ=255)

                       gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                                 >  2:5997961/1‑150 (MQ=255)

                        ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGATGTAAGGGGCCAGCG‑TAGCCGGAACTGACGCAATAGCGGGc                                                                                                                                >  1:353528/1‑150 (MQ=255)

                          ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                              <  2:1996520/150‑1 (MQ=255)

                            ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAA‑Gc                                                                                                                           >  2:1450492/1‑150 (MQ=255)

                                      ccTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGc                                                                                                                 >  2:5610052/1‑150 (MQ=255)

                                        tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAg                                                                                                               >  2:738482/1‑150 (MQ=255)

                                            gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑GAGCCGGAACTTAAGCAGTCGCGGGCAA‑GCCCCCACCAGCAggggg                                                                                                           >  1:3922413/1‑150 (MQ=255)

                                            gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCCAAGCCTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCTGCAggggg                                                                                                           >  2:4197127/1‑150 (MQ=255)

                                               ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGGGAGGTCAGGGGACCCCG‑TAGCCGGCACAGACGCAATCCCGGGCCA‑GCCCGCGCCAGCAGGGGGTAt                                                                                                        >  1:6162449/1‑150 (MQ=255)

                                                    ccTGTGCGGGGCGGGTGACAGAACATTAACCGATGATTGTCGGTTAAAAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCg                                                                                                   <  2:6229551/150‑1 (MQ=39)

                                                     cgggggggggCCGGTCACACAACATTAACCTTGGATGGTCGGTTAACACTGCAAATGGGGGGTGGCCGGCTATGGCTTGTGGTCCGGGGACAGGT‑TCGCCTGAACTGACGCATCCGGGGGCAC‑CCCCGCTCCTGCAGGGGGTATTGCCgg                                                                                                  <  2:2175859/144‑1 (MQ=255)

                                                        tgCGGGGCCGGTGACAGAACAGTACCCGATGTTTGGCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGAGGTCAGGGGACAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                               <  2:3952833/150‑1 (MQ=255)

                                                        tgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAAAGCGTGGGGTGAGGGGACAGCT‑TTGGCGGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCGGGCGGGGGGGTTGCCggggc                                                                                               >  1:4624211/1‑148 (MQ=255)

                                                           ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGGTTGCCGGGAAggg                                                                                            >  2:1893176/1‑150 (MQ=255)

                                                            gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTGCCGCGAATGCGTGAGGGGCGGGGGCAGCG‑TAGGCGGGACTGACGCAGTCGCGGGGAA‑GCCCCCGCCGGCCGGGGGGGTTGCCGGGGAgggg                                                                                           >  1:3626055/1‑149 (MQ=255)

                                                             ggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACAGACGCATTCGCGGGCAA‑GCCCGCTCCTTCAGGGGGGATTGCCGGGAAGGGtt                                                                                          >  2:3064226/1‑150 (MQ=255)

                                                               ccGGTTACACAACAGTACCCGATGATTGTCGGTTAAAAATGCAAACGGGGGCTGGCCCGCTAATGCTTGAGGTCAGGGGACAGCT‑TCGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                        <  2:2740326/150‑1 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCCAATTCGTGAGGTCAGGGGGCACCT‑TATCCGGAACGGACGCAATAGCGGGCCA‑CCCCCCGCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAg                                                                                >  1:111352/1‑150 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGACGGGTTTACGCCGAAg                                                                                >  1:5057228/1‑150 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAg                                                                                >  2:1560024/1‑150 (MQ=255)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTCTTGCCGGGCAGGGTTTAAGCCGTAg                                                                                >  2:6500934/1‑150 (MQ=39)

                                                                       aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCCCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCAGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTTCCGGGAAGGGTTTAAGCCGAAg                                                                                >  2:24955/1‑150 (MQ=255)

                                                                           ctgTACCCTATGTTTGTCGGGTAACAATGCAAACGCGGGCGTGCCCGCGATTGCGTGCGTTCAGGTTACAGCT‑TCGCCTGACCTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                            <  2:1057310/148‑1 (MQ=255)

                                                                           ctgTAACCGATGATGGTCGGTTAACAATGCAAATGCGGGCTGGCCCGCGATTGCGTGTGGCCTGGGGACAGCT‑TAGCCTGAACTGAGGCATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                            <  2:1057323/148‑1 (MQ=255)

                                                                              tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                         >  2:3837473/1‑150 (MQ=255)

                                                                              tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAATCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                         >  2:518363/1‑150 (MQ=255)

                                                                                aCCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGTGGTCTGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                       <  2:2805912/150‑1 (MQ=255)

                                                                                 ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCCCTCCTGCAGGGGGTATTGCCGGGAAGGGGTTAAGCCGCAGCCAGGTTCaa                                                                      >  2:1834126/1‑149 (MQ=255)

                                                                                   gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAtag                                                                    >  2:933672/1‑148 (MQ=255)

                                                                                   gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCCGGTTCATCg                                                                    >  2:1484756/1‑150 (MQ=255)

                                                                                   gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCGAAGCCAGGTTAAtag                                                                    >  2:229483/1‑148 (MQ=255)

                                                                                   gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGGGGGCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCAGGTTCaaag                                                                    >  2:229852/1‑147 (MQ=255)

                                                                                       ttggTCGGTAAACACTGCAACCGGGGGCTGGCCCGCTATTGGGTGCGGTCCGGGGACCGCT‑TCGCCTTACCTGCCGCATTCGGGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                                <  2:6062555/147‑1 (MQ=255)

                                                                                       atTGTCGGGTACCACCCCAAACGCGGGCTGGCCCGCTATTGGGTGCGGTCAGGGGACAGCT‑TCGCCTGAACTCCCCCATTCGGGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                                <  2:2639282/150‑1 (MQ=255)

                                                                                       atTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCCGGGGGTATTGCCGGGGAGGGTTTACGCCGAAGCCCGGTTCATCGCCtt                                                                >  2:4092368/1‑150 (MQ=255)

                                                                                         tGTCGGGAAACAATGAAAACGCGGGCTGGCCGGCGATTGCGTGTGTTCCGGGGACCGGT‑TCGCCTGACCTGACGAATGCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgt                                                              <  2:3922413/150‑1 (MQ=255)

                                                                                          gtgggttAACAATGCAAACGGGGGCTTGCCCGCGAATGCGTGCGGTCTGGGGACAGCT‑TAGCCTCAACGGACGAATCCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgtg                                                             <  2:4347900/144‑1 (MQ=255)

                                                                                          gTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGTCAGCT‑TAGCCTGAACGGTCGCGTTCGCGGGCAA‑GCCCGCTGCGGCAGGGGGTGTTGCCGGGCAGGGTTTACGCCGAAGCCAGGGTAATCGCCTtgtg                                                             >  2:5357049/1‑150 (MQ=255)

                                                                                           tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                            >  2:6791274/1‑150 (MQ=255)

                                                                                              gttaaataTGCAAAAGGGGGTTTGCCCGGTTTTGCTTGTGTTCGGGTTAATGAT‑TCGCCTCAAATGCCGAATTCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                         <  1:5711540/143‑1 (MQ=255)

                                                                                                         attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                              >  2:6105863/5‑150 (MQ=255)

                                                                                                                   ttGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaca                                    >  1:966987/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  1:2707981/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  1:1980240/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  1:4213730/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  2:5131755/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  1:5444030/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                 >  2:4581113/1‑150 (MQ=255)

                                                                                                                      cccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaacaag                                 >  1:4882449/1‑145 (MQ=18)

                                                                                                                       ccGCGAAGCCGTGAGGTCAGGGGACCGCT‑CACCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                <  2:5318345/150‑1 (MQ=255)

                                                                                                                        cgcgAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                               <  2:6588364/150‑1 (MQ=255)

                                                                                                                         gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                              >  1:3963743/1‑150 (MQ=255)

                                                                                                                          gttttGCTTGTGGTCGGGGGCCTGCT‑TCGCCCCAAGTGCCGC‑TTCCGGGGCAACCCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                             <  1:1061555/146‑1 (MQ=255)

                                                                                                                           gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                            >  1:2014613/1‑150 (MQ=255)

                                                                                                                               gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                             >  1:550024/1‑81 (MQ=255)

                                                                                                                                       tctggggACATCT‑TAGCCTTAACTGACGAATTCGCTGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCATGTGCTGTACCACACCCGTGTCAGCCAAGGtata                <  2:5054101/147‑4 (MQ=255)

                                                                                                                                       tccgggcACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                <  1:6778825/147‑1 (MQ=255)

                                                                                                                                       tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                >  2:6100636/1‑150 (MQ=255)

                                                                                                                                       tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                >  2:4841083/1‑150 (MQ=255)

                                                                                                                                       tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                >  2:2965814/1‑150 (MQ=255)

                                                                                                                                           gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata            >  1:1727633/1‑150 (MQ=255)

                                                                                                                                                aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc       >  2:1859506/1‑150 (MQ=255)

                                                                                                                                                 gCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                            >  1:3106697/1‑96 (MQ=255)

                                                                                                                                                  cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  1:5118258/1‑150 (MQ=255)

                                                                                                                                                  cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  1:2465942/1‑150 (MQ=255)

                                                                                                                                                  cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa     >  1:2864388/1‑150 (MQ=255)

                                                                                                                                                   t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                            >  1:446711/1‑94 (MQ=255)

                                                                                                                                                   t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg    >  2:4982820/1‑150 (MQ=255)

                                                                                                                                                      aGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGATGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAggg  >  2:5010776/1‑150 (MQ=255)

TGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGG  >  NC_002947/5102088‑5102386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`