BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,102,238	G→C	9.0%	intergenic (+232/+60)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,102,238	0	G	C	9.0%	430.1 / 27.8	155	intergenic (+232/+60)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (86/51); new base C (2/12); total (92/63)
Fisher's exact test for biased strand distribution p-value = 8.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01

Only 100 of 156 total aligned reads displayed.

TGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGC  >  NC_002947/5102091‑5102387

tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                      <  2:4914340/150‑1 (MQ=255)

tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                      <  1:6114791/150‑1 (MQ=255)

  aCTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                                    <  1:5936346/150‑1 (MQ=255)

    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGGATGCGTGAGGTCAGGGGACAGCT‑TAGCCTgta                                                                                                                                                  >  2:3163541/1‑148 (MQ=255)

    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                  <  2:5148412/150‑1 (MQ=255)

    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                                  <  2:6264366/150‑1 (MQ=255)

    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGACAGCG‑TAGccggga                                                                                                                                                  >  2:760574/1‑146 (MQ=255)

    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTCCGGGGCC‑GGTGACAGAACAGTAACCGATGACAGACGGGTAACAATGCAATTGCGGCCGTTCCCGCGAAAGCCTGAGGGCCCGGGACAGCT‑AAGccataa                                                                                                                                                  >  2:4034613/1‑146 (MQ=255)

         tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                             <  2:4340414/150‑1 (MQ=255)

         tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                             <  1:1470480/150‑1 (MQ=255)

         tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                             <  1:1470676/150‑1 (MQ=255)

         tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                             <  2:244196/150‑1 (MQ=255)

         tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                             <  1:5790312/150‑1 (MQ=255)

            aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCa                                                                                                                                          <  2:3896620/150‑1 (MQ=255)

            aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGCATGCGTGAGGTCAGGGGCCAGCT‑TAGCCTGAACTGACGCa                                                                                                                                          >  2:6778825/1‑150 (MQ=255)

                 aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc                                                                                                                                     <  1:2381229/150‑1 (MQ=21)

                  aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                    <  1:3005047/150‑1 (MQ=21)

                  aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                                                                    <  1:6001761/150‑1 (MQ=21)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  1:3416542/150‑1 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   >  2:3578442/1‑150 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  2:3673350/150‑1 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  2:3338779/150‑1 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  2:4732994/150‑1 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  2:6639158/150‑1 (MQ=255)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                   <  2:2122742/150‑1 (MQ=21)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                   <  1:4371051/150‑1 (MQ=21)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                                                                   <  1:2184440/150‑1 (MQ=21)

                   cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCGGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                                   <  1:1706720/150‑1 (MQ=255)

                    gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                                  >  2:5997961/1‑150 (MQ=255)

                       ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                               <  2:2287833/150‑1 (MQ=255)

                       ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCaa                                                                                                                               <  1:342006/150‑1 (MQ=21)

                        cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGAAGCATTAGCGGGCAA‑g                                                                                                                             >  2:1444565/1‑150 (MQ=255)

                         ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAA‑Gc                                                                                                                            >  2:1450492/1‑150 (MQ=255)

                          gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑Gcc                                                                                                                           <  2:1870415/150‑1 (MQ=255)

                                 ttCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGACCGCCACTGCGGGAGGTCAGGGGGCAGCG‑TAGCCGGAACCGACGCAGTCGCGGgccc‑gcccccaccg                                                                                                                    >  1:6488073/1‑144 (MQ=255)

                                   ccTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGc                                                                                                                  >  2:5610052/1‑150 (MQ=255)

                                     tGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAg                                                                                                                >  2:738482/1‑150 (MQ=255)

                                         gcCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑GAGCCGGAACTTAAGCAGTCGCGGGCAA‑GCCCCCACCAGCAggggg                                                                                                            >  1:3922413/1‑150 (MQ=255)

                                         gcCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCCAAGCCTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCGCGCCTGCAggggg                                                                                                            >  2:4197127/1‑150 (MQ=255)

                                            ccGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGCAAGGGGGAGGTCCGGGGCCAGCG‑TAGGCTGAACAGAACCATTAGCGGGCAA‑GCCCGCGCCAGCCggggggcg                                                                                                         >  1:621663/1‑147 (MQ=255)

                                            ccGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAAGCGGGAGGTCAGGGGACCCCG‑TAGCCGGCACAGACGCAATCCCGGGCCA‑GCCCGCGCCAGCAGGGGGTAt                                                                                                         >  1:6162449/1‑150 (MQ=255)

                                                 ccTGTGCGGGGCG‑GGTGACAGAACATTAACCGATGATTGTCGGTTAAAAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCg                                                                                                    <  2:6229551/150‑1 (MQ=39)

                                                        ggggCCGGGTCACACAACTGTACCGGATGTTTGTCGTTTAAAACTGCAAACGGGGGCTTGCCGGCCATTGGGTGTGGTACGGGTACAGCT‑TCGCCTGAAGGGACGATTTGGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                              <  2:1503259/150‑1 (MQ=255)

                                                        ggggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGGTTGCCGGGAAggg                                                                                             >  2:1893176/1‑150 (MQ=255)

                                                         gggCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTGCCGCGAATGCGTGAGGGGCGGGGGCAGCG‑TAGGCGGGACTGACGCAGTCGCGGGGAA‑GCCCCCGCCGGCCGGGGGGGTTGCCGGGGAgggg                                                                                            >  1:3626055/1‑149 (MQ=255)

                                                         ggccc‑GGTTAAAGAACCTTAACCTATTATTGTCGGTAAACAATGAAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTATGGGGACAGAT‑TACCCTGAACTGACGCATTCGCGGGAAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGt                                                                                            <  2:1612100/147‑1 (MQ=255)

                                                             cgggTGACATAACATTAACCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGTGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                         <  2:2740430/148‑1 (MQ=255)

                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCCAATTCGTGAGGTCAGGGGGCACCT‑TATCCGGAACGGACGCAATAGCGGGCCA‑CCCCCCGCCTGCAGGGGGTATTGCCGGGGAGGGTTTACGCCGAAg                                                                                 >  1:111352/1‑150 (MQ=255)

                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCAAGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGGATTGCCGGGACGGGTTTACGCCGAAg                                                                                 >  1:5057228/1‑150 (MQ=255)

                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTCTTGCCGGGCAGGGTTTAAGCCGTAg                                                                                 >  2:6500934/1‑150 (MQ=39)

                                                                         ctgTACCCTATGTTTGTCGGGTAACAATGCAAACGCGGGCGTGCCCGCGATTGCGTGCGTTCAGGTTACAGCT‑TCGCCTGACCTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAg                                                                             <  2:1057310/148‑1 (MQ=255)

                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                          >  2:5042795/1‑150 (MQ=255)

                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAATCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGtt                                                                          >  2:518363/1‑150 (MQ=255)

                                                                            tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCCCCAATGCCTGAGGTCAGGGGACACCT‑TAGCCGGAACTGACGCAATCGCCGGCAA‑GCCCCCGCCAGCACGGGGTAGTGCCCGGCAGGGTTTACGCCGAAGCCacggt                                                                          >  2:4620677/1‑146 (MQ=255)

                                                                              aCCGTTGTTTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGTGGTCTGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCa                                                                        <  2:2805912/150‑1 (MQ=255)

                                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAATCGTGAGGTAAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCCA‑GCCCCCTCCTGCAGGGGGTATTGCCGGGAAGGGGTTAAGCCGCAGCCAGGTTCaa                                                                       >  2:1834126/1‑149 (MQ=255)

                                                                                cGATGATTGTCGGGTAACAATGCAAAAGAGGGCTTGCCCGCGAATGCGTGTGGTCAGGGGACAGCA‑TAGCCAGAACTGACGCATTCGCGGGCAA‑GCCCGCACCAGCAGGGGGTCTGGCCGGGAAGGGTTTACGACGAAACCAGGGTCATc                                                                      >  1:5679547/1‑150 (MQ=255)

                                                                                 gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCAtag                                                                     >  2:933672/1‑148 (MQ=255)

                                                                                 gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAAGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCGAAGCCAGGTTAAtag                                                                     >  2:229483/1‑148 (MQ=255)

                                                                                     ttggTCGGTAAACACTGCAACCGGGGGCTGGCCCGCTATTGGGTGCGGTCCGGGGACCGCT‑TCGCCTTACCTGCCGCATTCGGGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                                 <  2:6062555/147‑1 (MQ=255)

                                                                                     atTGTCGGGTACCACCCCAAACGCGGGCTGGCCCGCTATTGGGTGCGGTCAGGGGACAGCT‑TCGCCTGAACTCCCCCATTCGGGGGCAA‑CCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCtt                                                                 <  2:2639282/150‑1 (MQ=255)

                                                                                       tGTCGGGAAACAATGAAAACGCGGGCTGGCCGGCGATTGCGTGTGTTCCGGGGACCGGT‑TCGCCTGACCTGACGAATGCGCGGGCAC‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgt                                                               <  2:3922413/150‑1 (MQ=255)

                                                                                        gtgggttAACAATGCAAACGGGGGCTTGCCCGCGAATGCGTGCGGTCTGGGGACAGCT‑TAGCCTCAACGGACGAATCCGGGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgtg                                                              <  2:4347900/144‑1 (MQ=255)

                                                                                        gTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGTCAGCT‑TAGCCTGAACGGTCGCGTTCGCGGGCAA‑GCCCGCTGCGGCAGGGGGTGTTGCCGGGCAGGGTTTACGCCGAAGCCAGGGTAATCGCCTtgtg                                                              >  2:5357049/1‑150 (MQ=255)

                                                                                         tCGGTTAACATTGCAAACGCGGGCTTGCCCGCTAATGCGTTAGGTCTGGGTACAGAT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             <  2:2119238/150‑1 (MQ=255)

                                                                                         tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             >  2:6791274/1‑150 (MQ=255)

                                                                                            ggTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTa                                                          >  2:2202833/1‑150 (MQ=255)

                                                                                                       attcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:6105863/5‑150 (MQ=255)

                                                                                                       aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg                                               >  2:3707831/1‑150 (MQ=255)

                                                                                                               gCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAA‑GCCCGCTTCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                       >  2:1024597/1‑150 (MQ=18)

                                                                                                                    cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                  >  2:5131755/1‑150 (MQ=255)

                                                                                                                    cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                  >  1:1980240/1‑150 (MQ=255)

                                                                                                                    cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                  >  1:2707981/1‑150 (MQ=255)

                                                                                                                    cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                                  >  1:4213730/1‑150 (MQ=255)

                                                                                                                    cccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACcaacaag                                  >  1:4882449/1‑145 (MQ=18)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                 >  1:5949125/1‑150 (MQ=255)

                                                                                                                     ccGCGAAGCCGTGAGGTCAGGGGACCGCT‑CACCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                                 <  2:5318345/150‑1 (MQ=255)

                                                                                                                      cgcgAATGCGTGAGGTCTGGGGACAGCT‑TCGCCTGAACTGCCGCTTTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                                <  2:6588364/150‑1 (MQ=255)

                                                                                                                       gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                               >  1:3963743/1‑150 (MQ=255)

                                                                                                                        gttttGCTTGTGGTCGGGGGCCTGCT‑TCGCCCCAAGTGCCGC‑TTCCGGGGCAACCCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGt                              <  1:1061555/146‑1 (MQ=255)

                                                                                                                         gAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTc                             >  1:2014613/1‑150 (MQ=255)

                                                                                                                             gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                              >  1:550024/1‑81 (MQ=255)

                                                                                                                             gCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAgg                                                                                              <  2:550024/81‑1 (MQ=255)

                                                                                                                                    gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg                  >  2:2404806/1‑150 (MQ=255)

                                                                                                                                     tctggggACATCT‑TAGCCTTAACTGACGAATTCGCTGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCATGTGCTGTACCACACCCGTGTCAGCCAAGGtata                 <  2:5054101/147‑4 (MQ=255)

                                                                                                                                     tccgggcACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                 <  1:6778825/147‑1 (MQ=255)

                                                                                                                                     tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                 >  2:2965814/1‑150 (MQ=255)

                                                                                                                                     tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                 >  2:6100636/1‑150 (MQ=255)

                                                                                                                                     tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga                 >  1:4518366/1‑150 (MQ=255)

                                                                                                                                     tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCAAAGGTCga                 >  2:4840930/1‑150 (MQ=255)

                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata             >  1:1727633/1‑150 (MQ=255)

                                                                                                                                              aGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc        >  2:2506915/1‑150 (MQ=255)

                                                                                                                                               gCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             <  2:3106697/96‑1 (MQ=255)

                                                                                                                                               gCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             >  1:3106697/1‑96 (MQ=255)

                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa      >  1:2864388/1‑150 (MQ=255)

                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa      >  1:2465942/1‑150 (MQ=255)

                                                                                                                                                cT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCa      >  1:5118258/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             >  1:446711/1‑94 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                                             <  2:446711/94‑1 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg     >  2:4982820/1‑150 (MQ=255)

TGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCC‑GGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAA‑GCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGC  >  NC_002947/5102091‑5102387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`