BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,186,149	A→T	5.1%	L264Q (CTG→CAG)	PP_4560 ←	ribonuclease BN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,186,149	0	A	T	5.1%	1081.2 / 28.7	312	L264Q (CTG→CAG)	PP_4560	ribonuclease BN
Reads supporting (aligned to +/- strand): ref base A (139/157); new base T (11/5); total (150/162)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

CTATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGCCC  >  NC_002947/5186002‑5186298

 tATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAgcagcagca                                                                                                                                                    <  2:2544908/150‑1 (MQ=255)

  aTGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCAc                                                                                                                                                   >  2:3324935/1‑150 (MQ=255)

  aTGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCAc                                                                                                                                                   <  1:1770891/150‑1 (MQ=255)

     aGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGc                                                                                                                                                >  2:1730635/1‑150 (MQ=255)

       gCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAg                                                                                                                                              >  2:119678/1‑150 (MQ=255)

        cTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGc                                                                                                                                             <  2:2175466/150‑1 (MQ=255)

        cTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGc                                                                                                                                             >  1:6401142/1‑150 (MQ=255)

         ttGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGcc                                                                                                                                            >  2:3665160/1‑150 (MQ=255)

         ttGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGcc                                                                                                                                            <  1:459468/150‑1 (MQ=255)

         ttGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCGGACATGGGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGcc                                                                                                                                            <  1:3061478/150‑1 (MQ=255)

             tCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGaaa                                                                                                                                        <  1:6563020/150‑1 (MQ=255)

             tCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGaaa                                                                                                                                        >  1:2795062/1‑150 (MQ=255)

              cGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAat                                                                                                                                       >  2:755199/1‑150 (MQ=255)

              cGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAat                                                                                                                                       >  1:2198502/1‑150 (MQ=255)

                ggTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAatat                                                                                                                                     >  2:5336402/1‑150 (MQ=255)

                 gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata                                                                                                                                    <  1:5116588/150‑1 (MQ=255)

                 gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata                                                                                                                                    <  2:2416091/150‑1 (MQ=255)

                 gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata                                                                                                                                    <  1:2448786/150‑1 (MQ=255)

                 gTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAAtata                                                                                                                                    <  1:4487271/150‑1 (MQ=255)

                  tttGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAg                                                                                                                                   >  1:6035327/1‑150 (MQ=255)

                    tGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGaa                                                                                                                                 <  1:3029072/150‑1 (MQ=255)

                       gTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATa                                                                                                                              >  2:6712597/1‑150 (MQ=255)

                        tGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATac                                                                                                                             >  1:4358177/1‑150 (MQ=255)

                         gCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATaca                                                                                                                            <  1:2607066/150‑1 (MQ=255)

                         gCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATaca                                                                                                                            <  1:2713453/150‑1 (MQ=255)

                         gCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATaca                                                                                                                            <  2:5074881/150‑1 (MQ=255)

                           tCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagc                                                                                                                          >  1:3995130/1‑150 (MQ=255)

                             gTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATacagcaa                                                                                                                        <  1:5573996/150‑1 (MQ=255)

                                     gTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACga                                                                                                                <  1:4904670/150‑1 (MQ=255)

                                       aCAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACgatg                                                                                                              <  1:764208/150‑1 (MQ=255)

                                        cAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACgatga                                                                                                             <  1:3798006/150‑1 (MQ=255)

                                        cAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACgatga                                                                                                             <  1:905235/150‑1 (MQ=255)

                                          gTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGAtt                                                                                                           <  2:4816289/150‑1 (MQ=255)

                                           tCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTg                                                                                                          >  1:4788660/1‑150 (MQ=255)

                                              aGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCAc                                                                                                       <  1:962811/150‑1 (MQ=255)

                                                gTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCa                                                                                                     <  1:1450667/150‑1 (MQ=255)

                                                gTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCa                                                                                                     >  1:6024553/1‑150 (MQ=255)

                                                      ttGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtgctg                                                                                               <  2:1823643/150‑1 (MQ=255)

                                                      ttGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAAtgctg                                                                                               >  2:4014782/1‑150 (MQ=255)

                                                        gggCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGcc                                                                                             <  2:3909602/150‑1 (MQ=255)

                                                          gCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGt                                                                                           >  1:1062932/1‑150 (MQ=255)

                                                          gCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGt                                                                                           >  1:4262060/1‑150 (MQ=255)

                                                            ttttCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTAc                                                                                         >  1:3511294/1‑150 (MQ=255)

                                                            ttttCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTAGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCAGTAc                                                                                         >  2:647773/1‑150 (MQ=255)

                                                                ccccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATgg                                                                                     <  2:4151151/150‑1 (MQ=255)

                                                                 cccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGc                                                                                    >  1:5343962/1‑150 (MQ=255)

                                                                 cccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGc                                                                                    >  2:5829112/1‑150 (MQ=255)

                                                                  ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCg                                                                                   <  1:5549165/150‑1 (MQ=255)

                                                                  ccGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCg                                                                                   >  2:6795022/1‑150 (MQ=255)

                                                                    gTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGtt                                                                                 <  1:2528199/150‑1 (MQ=255)

                                                                    gTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGtt                                                                                 <  2:1977302/150‑1 (MQ=255)

                                                                    gTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGtt                                                                                 <  1:1907217/150‑1 (MQ=255)

                                                                     tCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTg                                                                                <  1:4129617/150‑1 (MQ=255)

                                                                      cGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGt                                                                               <  2:38603/150‑1 (MQ=255)

                                                                       gAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTa                                                                              <  2:1566760/150‑1 (MQ=255)

                                                                       gAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTa                                                                              <  2:6086139/150‑1 (MQ=255)

                                                                        aaGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAg                                                                             >  1:3342937/1‑150 (MQ=255)

                                                                        aaGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAg                                                                             >  2:4716944/1‑150 (MQ=255)

                                                                          gTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTc                                                                           <  1:3869024/150‑1 (MQ=255)

                                                                           tCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCg                                                                          <  1:6650959/150‑1 (MQ=255)

                                                                            cTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCTGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCgg                                                                         <  2:2808771/150‑1 (MQ=255)

                                                                             tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAatga                                                                                                                                     >  2:2666673/1‑87 (MQ=21)

                                                                             tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCgg                                                                         <  1:5806022/149‑1 (MQ=255)

                                                                             tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGc                                                                        >  1:5016990/1‑150 (MQ=255)

                                                                             tttCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGc                                                                        >  1:5856553/1‑150 (MQ=255)

                                                                                 tcGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAg                                                                    >  2:5238548/1‑150 (MQ=255)

                                                                                    cccGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTc                                                                 <  2:2051283/150‑1 (MQ=255)

                                                                                     ccGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCt                                                                >  1:2036208/1‑150 (MQ=255)

                                                                                      cGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCtt                                                               >  1:4391572/1‑150 (MQ=255)

                                                                                      cGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCtt                                                               <  2:135192/150‑1 (MQ=255)

                                                                                       gggTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTc                                                              >  1:5753939/1‑150 (MQ=255)

                                                                                           tCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgt                                                          >  1:1685716/1‑150 (MQ=255)

                                                                                               gCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagta                                                      <  1:4648949/150‑1 (MQ=255)

                                                                                               gCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCAGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACgtagta                                                      <  2:6605274/150‑1 (MQ=255)

                                                                                                ccTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATATAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGtagtag                                                     >  1:4623339/1‑150 (MQ=255)

                                                                                                 cTTCTGCTGACATGTGCTCGATCACATCATTCATTTCGGC‑GCCGAGCAGCAGCACTTCAGCCGAAATATAGAAATACATCAACAGCACGATGATTGCACCAATGCTTCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAgg                                                    <  1:544507/150‑1 (MQ=255)

                                                                                                  ttCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGaaa                                                                                                                                        >  2:6593882/1‑65 (MQ=14)

                                                                                                  ttCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGc                                                   >  1:125734/1‑150 (MQ=255)

                                                                                                      gCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAg                                               <  2:592981/150‑1 (MQ=255)

                                                                                                            aTGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAgg                                         <  2:4291502/150‑1 (MQ=255)

                                                                                                             tgtgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAggg                                        <  2:6643099/150‑1 (MQ=255)

                                                                                                              gtgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGa                                       <  2:3471148/150‑1 (MQ=255)

                                                                                                               tgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAg                                      <  1:482145/150‑1 (MQ=255)

                                                                                                               tgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAg                                      >  1:6193546/1‑150 (MQ=255)

                                                                                                               tgCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAg                                      >  1:1162406/1‑150 (MQ=255)

                                                                                                                gCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAgg                                     >  2:4547834/1‑150 (MQ=255)

                                                                                                                 cTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGc                                    <  2:258360/150‑1 (MQ=255)

                                                                                                                  tCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGcc                                   >  2:4948372/1‑150 (MQ=255)

                                                                                                                        aCCGCATTCATTTCGGC‑GCCGAGCAGCAGCACTGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATc                             <  2:5463375/150‑1 (MQ=255)

                                                                                                                        aCCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATc                             <  1:4972630/150‑1 (MQ=255)

                                                                                                                        aCCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATc                             <  1:610947/150‑1 (MQ=255)

                                                                                                                         ccGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATcc                            >  2:5299763/1‑150 (MQ=255)

                                                                                                                          cGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATcca                           >  2:3554255/1‑150 (MQ=255)

                                                                                                                             attcattTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacac                        >  2:4211605/1‑150 (MQ=255)

                                                                                                                              tatattTCGGCTGCCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATccacacc                       >  1:631007/4‑150 (MQ=38)

                                                                                                                                     cggctgcCGTGCTGCTGCTCGGC‑GCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCg                >  2:1180531/6‑150 (MQ=255)

                                                                                                                                     cGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCg                >  1:2888752/1‑150 (MQ=255)

                                                                                                                                      ggC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGc               <  1:6712597/150‑1 (MQ=255)

                                                                                                                                            cGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCa          <  1:2283175/150‑1 (MQ=255)

                                                                                                                                                    agcaCGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGccc  >  2:618659/1‑150 (MQ=255)

CTATGAGGCTTGCTCGGGTTTGGGTGCTCGTGACCCAGTACAGTCAAGGTTTCCTTGGGCTTTTCCCCGTCGAAGTCTTTCTCGCCCGGGTTCTTGCCTTCGGCTGACATGTGCTCGATCACCGCATTCATTTCGGC‑GCCGAGCAGCAGCACGGCAGCCGAAATATAGAAATACAGCAACAGCACGATGATTGCACCAATGCTGCCGTACATGGCGTTGTAGTCGGCAAAGGTCTTCACGTAGTAGGCAAAGCCCAGGGAGGCCACGATCCACACCACAACCGCCAGCACCGAGCCC  >  NC_002947/5186002‑5186298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`