BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,189,114	C→T	6.9%	A85V (GCC→GTC)	PP_4564 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,189,114	0	C	T	6.9%	946.0 / 39.2	305	A85V (GCC→GTC)	PP_4564	lipoprotein
Reads supporting (aligned to +/- strand): ref base C (146/138); new base T (8/13); total (154/151)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 310 total aligned reads displayed.

GGTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGG  >  NC_002947/5188965‑5189260

ggTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGc                                                                                                                                                    <  2:381923/150‑1 (MQ=255)

   caggtagTCGCTCAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcga                                                                                                                                                 >  2:3650206/7‑150 (MQ=255)

      gTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgacga                                                                                                                                              <  1:2966708/150‑1 (MQ=255)

       tGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCgacgac                                                                                                                                             >  1:3157207/1‑150 (MQ=255)

          tCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg                                                                                                                                          <  1:1682305/150‑1 (MQ=255)

            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGt                                                                                                                                        <  1:2113374/150‑1 (MQ=255)

            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                        <  1:121185/150‑1 (MQ=255)

            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                        <  1:4262227/150‑1 (MQ=255)

            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                        <  1:6677346/150‑1 (MQ=255)

            gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                        >  2:3196528/1‑150 (MQ=255)

             cTGAGCGACTACCTGGACATGAACCGCGAGCAACGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                       <  2:6711614/150‑1 (MQ=255)

             cTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                       <  1:2205026/150‑1 (MQ=255)

             cTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                       <  2:3724762/150‑1 (MQ=255)

              tGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCa                                                                                                                                      <  2:618217/150‑1 (MQ=255)

              tGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGTCGGCGACCATGTCa                                                                                                                                      <  1:39580/150‑1 (MQ=255)

               gAGCGACTCCCTCGACCCGACCCGCGAGCCCCGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCAc                                                                                                                                     <  2:6362648/150‑1 (MQ=255)

               gAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAc                                                                                                                                     <  1:1622762/150‑1 (MQ=255)

                  cGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCAcgta                                                                                                                                  <  1:487187/150‑4 (MQ=255)

                      tACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAg                                                                                                                              <  2:2467226/150‑1 (MQ=255)

                        ccTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGc                                                                                                                            >  2:6090433/1‑150 (MQ=255)

                         cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc                                                                                                                           >  1:2387025/1‑150 (MQ=255)

                         cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc                                                                                                                           <  1:940263/150‑1 (MQ=255)

                          tGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGccc                                                                                                                          >  2:2693426/1‑150 (MQ=255)

                            gACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTg                                                                                                                        <  1:3025631/150‑1 (MQ=255)

                             aCATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACGGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgc                                                                                                                       <  2:4592397/150‑1 (MQ=255)

                              cATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgca                                                                                                                      <  2:5575624/150‑1 (MQ=255)

                                 gAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgcagca                                                                                                                   >  1:4359887/1‑150 (MQ=255)

                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                 <  2:1892237/150‑1 (MQ=255)

                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                 <  1:408923/150‑1 (MQ=255)

                                   aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa                                                                                                                 <  1:6225957/150‑1 (MQ=255)

                                      gcgAGCACAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCa                                                                                                              <  2:1037647/150‑1 (MQ=255)

                                         aGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCAccc                                                                                                           <  1:639890/150‑1 (MQ=255)

                                            aaaGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCcgcg                                                                                                        >  1:1845475/1‑150 (MQ=255)

                                              aGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAg                                                                                                      <  2:2143413/150‑1 (MQ=255)

                                                ggCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGt                                                                                                    >  2:5341210/1‑150 (MQ=255)

                                                    ctgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCACAGCACCCGCGAGGTCCGt                                                                                                >  2:5186317/1‑150 (MQ=255)

                                                    ctgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                <  1:3355807/150‑1 (MQ=255)

                                                    ctgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                <  2:1505796/150‑1 (MQ=255)

                                                      gctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCGGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                              <  2:254525/150‑1 (MQ=255)

                                                      gctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                              >  1:2780008/1‑150 (MQ=255)

                                                        tgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                            >  1:4984108/1‑150 (MQ=255)

                                                                cGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGt                                                                                    <  1:4427749/150‑1 (MQ=255)

                                                                     gcgcGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                               >  2:506314/1‑150 (MQ=255)

                                                                      cgcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTg                                                                              <  2:4514639/150‑1 (MQ=255)

                                                                            cACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAg                                                                        >  1:5836726/1‑150 (MQ=255)

                                                                             aCCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                                       >  1:5477316/1‑150 (MQ=255)

                                                                             aCCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                                       >  1:2621012/1‑150 (MQ=255)

                                                                              cctggcctggCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGa                                                                      <  1:1457458/150‑1 (MQ=255)

                                                                                  gcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATc                                                                  <  2:2186837/150‑1 (MQ=255)

                                                                                    ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                >  2:2844130/1‑150 (MQ=255)

                                                                                     tggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                               <  1:5026978/150‑1 (MQ=255)

                                                                                     tggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                               >  1:5297821/1‑150 (MQ=255)

                                                                                      ggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAccc                                                              <  1:2738961/150‑1 (MQ=255)

                                                                                          ttGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTc                                                          <  1:5065297/150‑1 (MQ=255)

                                                                                             cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGACATCACCCCTTCGGc                                                       >  1:4687386/1‑150 (MQ=255)

                                                                                              aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                      >  2:2247124/1‑150 (MQ=255)

                                                                                              aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                      >  2:1275866/1‑150 (MQ=255)

                                                                                              aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                      >  1:3472007/1‑150 (MQ=255)

                                                                                              aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                      >  1:3827338/1‑150 (MQ=255)

                                                                                              aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGcc                                                      >  1:6479220/1‑150 (MQ=255)

                                                                                               aGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCa                                                     >  1:793032/1‑150 (MQ=255)

                                                                                                 aCTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                                   >  2:1447460/1‑150 (MQ=255)

                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGa                                                 >  2:5704750/1‑150 (MQ=255)

                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGa                                                 >  2:4212251/1‑150 (MQ=255)

                                                                                                   tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGa                                                 >  2:3276037/1‑150 (MQ=255)

                                                                                                     aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc                                               <  1:1325234/150‑1 (MQ=255)

                                                                                                     aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc                                               >  2:5541311/1‑150 (MQ=255)

                                                                                                       cTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActg                                             >  2:1774909/1‑150 (MQ=255)

                                                                                                        tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAACTCACCCCTTCGGCCACCGAActgc                                            >  2:6306075/1‑150 (MQ=255)

                                                                                                        tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                         <  1:1668752/137‑1 (MQ=255)

                                                                                                         gCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgct                                           <  2:4430976/150‑1 (MQ=255)

                                                                                                           ccGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACtgctgc                                         >  2:1153308/1‑150 (MQ=255)

                                                                                                           ccGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACtgctgc                                         >  2:3920714/1‑150 (MQ=255)

                                                                                                                 aCCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCa                                   <  1:6582899/150‑1 (MQ=255)

                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATg                                 >  2:5794306/1‑150 (MQ=255)

                                                                                                                   cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATg                                 >  2:2609031/1‑150 (MQ=255)

                                                                                                                    tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                >  1:1941276/1‑150 (MQ=255)

                                                                                                                    tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                >  1:4927354/1‑150 (MQ=255)

                                                                                                                    tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa                                <  1:3637524/150‑1 (MQ=255)

                                                                                                                     cGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAg                               <  2:2019227/150‑1 (MQ=255)

                                                                                                                      agggggCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGc                              <  2:1688363/146‑1 (MQ=255)

                                                                                                                         tGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGAc                           >  1:875454/1‑150 (MQ=255)

                                                                                                                            cTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAc                        <  2:4276218/150‑1 (MQ=255)

                                                                                                                                 ccGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGt                   >  2:807422/1‑150 (MQ=255)

                                                                                                                                 ccGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGt                   <  1:2647238/150‑1 (MQ=255)

                                                                                                                                       aCGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCa             <  2:5007391/150‑1 (MQ=255)

                                                                                                                                       aCGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCa             >  2:3023437/1‑150 (MQ=255)

                                                                                                                                        ggTGACCTGGTCGTCGGCGACCATGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCACTTTGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAg            >  2:4704105/2‑150 (MQ=255)

                                                                                                                                          tGACCTGGTCGTCGGCGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGAt          >  2:652297/1‑150 (MQ=255)

                                                                                                                                           gACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATg         <  1:1125736/150‑1 (MQ=255)

                                                                                                                                              aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt      >  2:4053714/1‑150 (MQ=255)

                                                                                                                                              aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGATCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt      <  2:3440069/150‑1 (MQ=255)

                                                                                                                                               tGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTg     >  2:5397671/1‑150 (MQ=255)

                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa    <  2:927888/150‑1 (MQ=255)

                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa    >  2:6280422/1‑150 (MQ=255)

                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa    >  2:4878626/1‑150 (MQ=255)

                                                                                                                                                ggTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGa    <  1:6090433/150‑1 (MQ=255)

                                                                                                                                                 gTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAg   <  1:1544891/150‑1 (MQ=255)

                                                                                                                                                  tCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTCGCGCAGATGCGTGAgg  >  2:6431853/1‑150 (MQ=255)

                                                                                                                                                  tCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAgg  <  1:3273941/150‑1 (MQ=255)

GGTGCCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGG  >  NC_002947/5188965‑5189260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`