Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,199,885 C→A 10.8% intergenic (+30/+23) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,199,8850CA10.8% 690.0 / 63.5 242intergenic (+30/+23)PP_4574/PP_4575hypothetical protein/urea amidolyase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (126/90);  new base A (15/11);  total (141/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01

Only 100 of 245 total aligned reads displayed.

CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCGC  >  NC_002947/5199736‑5200033
                                                                                                                                                     |                                                                                                                                                    
cccAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTc                                                                                                                                                      >  2:5833213/1‑150 (MQ=255)
 ccAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCg                                                                                                                                                     >  2:3730103/1‑150 (MQ=255)
    aGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGccc                                                                                                                                                  <  2:5165053/150‑1 (MQ=255)
     gCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCcg                                                                                                                                                 >  2:3813870/1‑150 (MQ=255)
       aGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCcgcg                                                                                                                                               <  2:6579605/150‑1 (MQ=255)
        ggCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGa                                                                                                                                              <  1:1584836/150‑1 (MQ=255)
          ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGCTGTATCCGCTGACCCAGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGaaa                                                                                                                                            <  1:3199380/150‑1 (MQ=255)
          ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGaaa                                                                                                                                            <  1:531202/150‑1 (MQ=255)
          ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                            <  1:2001544/150‑1 (MQ=255)
          ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                            <  1:637472/150‑1 (MQ=255)
          ccTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                            <  1:3848068/150‑1 (MQ=255)
           cTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACTGGCCCTTTCGCGGGCGAGCCCGCGAAAg                                                                                                                                           <  1:1020683/150‑1 (MQ=255)
           cTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCGACCTGTCCAAGTGAGGTTGCCTGTACTGGCCCTTTCGCGGGCGAGCCCGCGAAAg                                                                                                                                           <  2:4167642/150‑1 (MQ=255)
            tGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAgg                                                                                                                                          <  1:400410/150‑1 (MQ=255)
              ggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGc                                                                                                                                        >  1:5848802/1‑150 (MQ=255)
                aGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCca                                                                                                                                      <  2:2954298/150‑1 (MQ=255)
                 gTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcag                                                                                                                                     <  2:3367005/150‑1 (MQ=255)
                 gTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcag                                                                                                                                     >  1:833446/1‑150 (MQ=255)
                   acacCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcagca                                                                                                                                   <  2:5753092/150‑1 (MQ=255)
                     acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGt                                                                                                                                 >  2:5239984/1‑150 (MQ=255)
                     acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGt                                                                                                                                 >  1:4506080/1‑150 (MQ=255)
                     acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGt                                                                                                                                 >  2:6848969/1‑150 (MQ=255)
                      ccGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTc                                                                                                                                >  1:2902036/1‑150 (MQ=255)
                      ccGCCTCGACCCATCTGGGGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGTGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACTGGCCCTTTCGCGGGCGAGCCCGCGAAAGGGccggtacag                                                                                                                                <  2:6013411/150‑8 (MQ=38)
                        gCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCtt                                                                                                                              <  1:6241388/150‑1 (MQ=255)
                          cTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTAc                                                                                                                            <  2:2709441/150‑1 (MQ=255)
                          cTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTAc                                                                                                                            >  1:1417520/1‑150 (MQ=255)
                          cTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTAc                                                                                                                            <  2:936783/150‑1 (MQ=255)
                              aCCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAcc                                                                                                                        <  2:3687062/150‑1 (MQ=255)
                               cccATCTGGGGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCACGTGAGGTTGCCTGTCCCGGCCCTTGCGCGGGCGCGCCCGCGAAAGGGCCAGCAGTCTTACGACCa                                                                                                                       <  1:167037/150‑1 (MQ=255)
                                 cATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAAc                                                                                                                     <  1:5452686/150‑1 (MQ=255)
                                  aTCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCCGCAGTCTTACGACCAACg                                                                                                                    >  1:1239942/1‑150 (MQ=255)
                                  aTCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACg                                                                                                                    >  1:4349480/1‑150 (MQ=255)
                                                  gATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgc                                                                                                    <  2:2797210/150‑1 (MQ=255)
                                                   aTCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgcggct                                                                                                   >  2:2493840/1‑150 (MQ=255)
                                                    tCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgctg                                                                                                  >  2:3216294/1‑150 (MQ=255)
                                                           cAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCCGCAGTCTTACCACCAACGCCCCAGGTAAAGCTGCTGCTCTCGc                                                                                           >  1:901181/1‑150 (MQ=255)
                                                           cAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGc                                                                                           <  1:5117857/150‑1 (MQ=255)
                                                            aaGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCAGCAGGCGTACGCCCAACGCCCCAGGTAAAGCGGCTGCTCTCGcc                                                                                          >  1:2607248/1‑150 (MQ=255)
                                                              gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                        <  1:6705595/150‑1 (MQ=255)
                                                                   aTCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTc                                                                                   >  2:6109829/1‑150 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCg                                                                                  >  1:2801389/1‑150 (MQ=255)
                                                                       gCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTcc                                                                               >  2:1343832/1‑150 (MQ=255)
                                                                       gCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTcc                                                                               <  2:833446/150‑1 (MQ=255)
                                                                        cTGACCCTGCATGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCt                                                                              <  1:329436/150‑1 (MQ=255)
                                                                        cTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCt                                                                              <  1:3465541/150‑1 (MQ=255)
                                                                          gACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGc                                                                            >  2:6484033/1‑150 (MQ=255)
                                                                             ccTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcacc                                                                         >  1:6268272/1‑150 (MQ=255)
                                                                              cTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGGAAGGGCCAGCAGTCGTACGGCCAACGCCCCAGGTAAAGCTGCTGCGCTCGCCAGGCCGCGTCCTGcacca                                                                        >  1:3215113/1‑150 (MQ=255)
                                                                              cTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcacca                                                                        >  1:2010623/1‑150 (MQ=255)
                                                                               tGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGcaccac                                                                       >  2:1757506/1‑150 (MQ=255)
                                                                                         ggacgccGGGATGCGCTACCTGTCCAAGGCAGGTTGCCTGTACCGGCCCTCTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAAc                                                             <  1:116027/144‑1 (MQ=255)
                                                                                             gaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTGGCGGGCTCGCCCCCGCAAGGGCCCGCAGGCTTACGACCCACGCCCCCGGTAAAGCGGCTGCGCTCGCCCGGCCACGGCCGGCACCACCCCCCGGAAccccc                                                         >  1:783335/1‑147 (MQ=255)
                                                                                                 ggATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGcaccac                                                     <  1:5393494/150‑1 (MQ=255)
                                                                                                  gATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCTAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGc                                                                            <  1:2275172/126‑1 (MQ=255)
                                                                                                  gATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGc                                                                            >  2:2275172/1‑126 (MQ=255)
                                                                                                  gATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGATCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGTACCGCaccacc                                                    >  2:3526242/1‑150 (MQ=255)
                                                                                                        cTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCACCAGTCTTACGACCAACGCCCCACGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCAGCACCACCCCCCAGAACCCCACCACCCTcccc                                              >  1:5648528/1‑150 (MQ=255)
                                                                                                        cTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTcccc                                              <  2:4645591/150‑1 (MQ=255)
                                                                                                           ccTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCCGGCCTCGTCCTGCACCACCGCCCTGACCCGCACCACCGTCCCCCGc                                           >  1:4942416/1‑150 (MQ=255)
                                                                                                            cTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGACa                                          >  1:5067760/1‑150 (MQ=255)
                                                                                                               tCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGc                                       >  2:430069/1‑150 (MQ=255)
                                                                                                                 cAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCAGCAGTCTTACGACCAACGCCCCACGGAAAGCAGCAGCTCTCGCCAGGCCTCGTCCAGCACCACCGCCCAGAACCCCACCCCCCTCCCCGGCCAGCAc                                     >  1:6658076/1‑150 (MQ=255)
                                                                                                                 cAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGCGCCCGCGCAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCGCGTCCTGCACCACCGCCCAGAACCGCACCACCCTCCCCGGCATGCAc                                     >  1:2629337/1‑150 (MQ=255)
                                                                                                                    gTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTgc                                  >  2:2110446/1‑150 (MQ=255)
                                                                                                                    gTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCACTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTgc                                  >  2:311873/1‑150 (MQ=255)
                                                                                                                         gTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAg                             <  2:5464850/150‑1 (MQ=255)
                                                                                                                          ttGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCGTTCGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCCGGCCTCGTCCTGCCCCCCCGCCCTGAACCGCCCCACCGTCCCCGGCATGCCCTGCGCCAGt                            >  2:158896/1‑150 (MQ=255)
                                                                                                                             ccTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgc                         <  2:5024063/150‑1 (MQ=255)
                                                                                                                               tGTACCGGCCCTTTCGCGGGCTCGCCCGCGCAAGGGCCAGCACTCGTACGACCAACGCCCCCCGTAAAGCGGCGGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCAGAACCGCACCACCGTCCCCCGCAAGCACTGCGCCAGTTtcgc                       >  2:21356/1‑150 (MQ=255)
                                                                                                                                gTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgcc                      <  2:3437640/150‑1 (MQ=255)
                                                                                                                                 tACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgcca                     <  2:1846219/150‑1 (MQ=255)
                                                                                                                                  aCCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCGTACGACCAACGCCCCAGGTAAAGCAGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCCCCCGGAACCGCACCACCGTCCCCGGCATGCACAGCGCCAGTTgcgcccg                    >  1:2167799/1‑148 (MQ=255)
                                                                                                                                   ccGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagc                   <  1:3961382/150‑1 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgcc                >  2:1865504/1‑150 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTAAGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgcc                >  1:3422576/1‑150 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgcc                >  1:3248230/1‑150 (MQ=255)
                                                                                                                                         cTTTCGCGGGCTCGCCCGCTAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc             <  1:2125744/150‑1 (MQ=255)
                                                                                                                                         cTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc             >  2:3800263/1‑150 (MQ=255)
                                                                                                                                         cTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCgccagcgccagc             >  2:4550390/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  2:6329294/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  2:4952468/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  2:657694/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  1:4234048/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCGAGCCCGCGAAAGGGGCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  2:1458458/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  2:4281469/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  1:5746044/1‑150 (MQ=255)
                                                                                                                                          tttCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg            >  1:6075933/1‑150 (MQ=255)
                                                                                                                                            tCGCTGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc          <  2:304748/150‑1 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGATAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc          <  2:2131869/150‑1 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc          <  2:4506080/150‑1 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc          <  1:3730103/150‑1 (MQ=255)
                                                                                                                                            tCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggc          <  2:6196304/150‑1 (MQ=255)
                                                                                                                                              gcgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGt        >  1:5250038/1‑150 (MQ=255)
                                                                                                                                               cgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTc       <  2:5848802/150‑1 (MQ=255)
                                                                                                                                                gggCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCa      >  2:6801367/1‑150 (MQ=255)
                                                                                                                                                gggCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCa      <  1:1810877/150‑1 (MQ=255)
                                                                                                                                                  gCTCGCCCTCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgc    <  2:2902036/150‑1 (MQ=255)
                                                                                                                                                  gCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgc    <  2:2010623/150‑1 (MQ=255)
                                                                                                                                                   cTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcg   >  2:3938238/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCGC  >  NC_002947/5199736‑5200033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑