Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,451,289 T→G 11.6% L146R (CTC→CGC)  thiE → thiamine‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,451,2890TG11.6% 516.6 / 7.3 267L146R (CTC→CGC) thiEthiamine‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (127/94);  new base G (32/7);  total (161/106)
Fisher's exact test for biased strand distribution p-value = 3.98e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GGCCGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCC  >  NC_002947/5451141‑5451438
                                                                                                                                                    |                                                                                                                                                     
    gTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGc                                                                                                                                                  <  2:1155230/150‑1 (MQ=255)
     tCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGcc                                                                                                                                                 >  2:1959732/1‑149 (MQ=255)
       aGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAgg                                                                                                                                               >  1:903214/1‑150 (MQ=255)
         gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGcc                                                                                                                                             >  2:739541/1‑150 (MQ=255)
         gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGGGCGGGcc                                                                                                                                             >  1:6192732/1‑150 (MQ=255)
         gCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGAGCAGGcc                                                                                                                                             >  2:819054/1‑150 (MQ=255)
            atcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCTGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCcgc                                                                                                                                          <  2:4432304/150‑1 (MQ=255)
            atcatcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGGGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCcgc                                                                                                                                          <  2:430885/150‑1 (MQ=255)
               atcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCGGTGGCCGCTGCTGCAAGTCTGTCACCAAGCCGGGCGCGCCCGCTGCCCCCGTCGGCCGGCGCGCGCCGGCCCGCGcc                                                                                                                                       >  2:4385468/1‑150 (MQ=255)
                tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGACGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCCGGCCCGCGccc                                                                                                                                      >  2:5748498/1‑150 (MQ=255)
                tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGGCTGCGCGCGCCGGCCCGCGccc                                                                                                                                      >  1:4184769/1‑150 (MQ=255)
                tcGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGccc                                                                                                                                      >  1:5573089/1‑150 (MQ=255)
                acGGCTCCACCTGCCACGCCACCATCGAACGGCCCGCCCAGGCGGCCAGCGAAGCCGCCCGCGACGTGGCATTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCCCCGACGGCCTGCTCGAGCAGGGCCGCGccc                                                                                                                                      <  2:636966/149‑1 (MQ=255)
                   gCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCacg                                                                                                                                   >  1:1121111/1‑148 (MQ=255)
                    cTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCacgg                                                                                                                                  >  1:2701424/1‑147 (MQ=255)
                       cACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAGGCCCCCGCCCAGGTGaa                                                                                                                               >  2:5721808/1‑150 (MQ=255)
                         ccTGCCACGCCTGCCTCTAAGGGGCCGCCCAGGCGGCCAGGGATGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCATTTCTGTCACCCAGCCGGGCGCGCCCGCGGCCACCGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAAc                                                                                                                             <  2:2266829/150‑1 (MQ=255)
                         ccTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCCGGCCCGCGCCCACGGGAAAc                                                                                                                             >  1:3779506/1‑150 (MQ=255)
                          cTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACt                                                                                                                            >  2:4748154/1‑150 (MQ=255)
                          cTGCCACGCCAGCCTCCAACTCGCCGCCCAGGCTGCCCGCGAAGGCGCCAGCTACGAGGCCTTTGGCCGCTTCTTCAACTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGCCCTGCTCGAGCAGGCCCGCCCCCAGCTGCAAca                                                                                                                            >  2:6055609/1‑149 (MQ=255)
                            gCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCCCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGc                                                                                                                          <  2:5843327/150‑1 (MQ=255)
                            gCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGc                                                                                                                          <  2:4522483/150‑1 (MQ=255)
                                   cAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCgcgc                                                                                                                                        <  1:6831312/129‑1 (MQ=255)
                                   cAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCgcgc                                                                                                                                        >  2:6831312/1‑129 (MQ=255)
                                        tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGGGa                                                                                                              >  1:6594196/1‑150 (MQ=255)
                                        tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCCTGa                                                                                                              >  2:3463288/1‑150 (MQ=255)
                                        tCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCTCGAGCACGCCCGCGCCCAGGTGACACAGCCCCTCGCCgggc                                                                                                              >  1:6654604/1‑147 (MQ=255)
                                        cttcgcTGGCAGCCTGGGCGGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGGACGCCGTgc                                                                                                              >  2:2050067/6‑149 (MQ=255)
                                              tGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTg                                                                                                        >  1:5536343/1‑150 (MQ=255)
                                              tGGCAGCCTGGGCGGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTg                                                                                                        >  2:5023205/1‑150 (MQ=255)
                                                           tGCCAGCGACGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCCGGCCCGCGCCCAGGTGAAACGGCCGAGCGCCGGGATCGGTGGCAGCACCCTCGa                                                                                           >  2:5961923/1‑150 (MQ=255)
                                                             ccAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACa                                                                                         >  2:2914063/1‑150 (MQ=255)
                                                              cAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACaa                                                                                        >  2:2412403/1‑150 (MQ=255)
                                                                gCGAAGGCGCCAGCTACGTTGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGAGCGCCCTCTGCCAACGTCGGCCAGCTCGAGAAGGCCCGCGACCAGGAGTAACTGCCCATCCACGGGATAGGTGGCATCAACCAAGACaaaa                                                                                      >  1:4990244/1‑148 (MQ=255)
                                                                gCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCGCCAGCAGGCCCGCCCCCAGGAGAAACAGCCGATCGCCGTGATCGGTGGCATCACCCGCGACAAcg                                                                                      >  2:3905387/1‑150 (MQ=255)
                                                                 cgccggCGCCAGCTACGGGGCCTTGGGCCGCTTCTTCAATTCTGTCACCACGCCGGGCGCGCCCGCGGCCACCGTCGGCCGGCCCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgc                                                                                     <  2:277654/146‑1 (MQ=255)
                                                                 cGATGGCGCCAGCTCCGTGGCCTTTGGCCGCTTCTTCAATTCTTTCACCTCGCCGGGCGCGCCCGCTGCCACCGGCGGCCGGCCCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgc                                                                                     <  1:1507899/150‑1 (MQ=255)
                                                                  gacggCGCCAGCTAGGTGGCCTTTGGCCGCTTCTTCAATTCGGTCACCACGCGGGGCGCGCCCGCCCCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgcc                                                                                    <  1:2745880/147‑1 (MQ=255)
                                                                  gAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCAGGCCCGCGCCCCGGTGAAACAGCCCCTCGCCGTGATCGGTGGCATCACCCACGACAAcccc                                                                                    >  1:4293031/1‑150 (MQ=255)
                                                                  gAAGGCGCCAGCTACGTGGCCTTGGGCCGCTTCTGCAAGTCGGTCACCACGCCGGGCGCGCCCGCGGCCAACGTCGGCCTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgcc                                                                                    <  2:5193888/150‑1 (MQ=255)
                                                                    cggCGCCAGCTCCGGGGCCTCTGGCCGCTTCTACAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGGCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAAcgccgc                                                                                  <  2:66587/149‑1 (MQ=255)
                                                                     ggCGCCCGCTACGTGGCCTTTGGCGGCTTCTTCACTTCTGTCCCCACGCGGGGCGCGCCCGCGGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACgccgcc                                                                                 <  1:4476923/150‑1 (MQ=255)
                                                                      gcgcCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGccc                                                                                >  2:202848/1‑150 (MQ=255)
                                                                         ccAGCTACGGGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGCCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCAc                                                                             <  2:411119/150‑1 (MQ=255)
                                                                           aGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCCTCACCCTCGACAACGCCGCCCCACTg                                                                           >  2:5106860/1‑150 (MQ=255)
                                                                              tACGTGGCCTTT‑GCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGCTCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACAGGTCg                                                                       >  2:3554893/1‑150 (MQ=255)
                                                                                ggTGGCCTGTGGCCGCTAATACATTTCGGCCACCCCGCCGGGCGCGCCCGCCCCCACCGCCGGCCTGCTCGCGCAGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGc                                                                      <  1:1959732/149‑1 (MQ=255)
                                                                                  tGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGccc                                                                    >  2:4586772/1‑150 (MQ=255)
                                                                                  tGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGAGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGccc                                                                    >  2:6817310/1‑150 (MQ=255)
                                                                                  tGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCAGCTCGAGCAGGCCCCCGCCCAGGTGAAACTGCCGATCGCCCTGATCGGTGGCATAACCCTCGACAACGCCGCCCCACAGGTCGccc                                                                    >  2:241656/1‑150 (MQ=255)
                                                                                   ggCCTTTGGCCGCTTCTTCACTTCGGTCACCACGCCGGGCGCGCCCGCTGCCACCGGCGGCCTGCTCGAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCa                                                                   <  2:1352571/150‑1 (MQ=255)
                                                                                      cTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCACGAGCAGGCCCGCCCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCAACACCCACGACAACGCCGCCCCACTGGTCCCCCACgg                                                                >  2:5000815/1‑150 (MQ=255)
                                                                                         tGGCCGCTTCTTCAATTCTGTCCCCACGCGGGGCGCGCCCGCTGCCACCGTGGGCCTGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGgcgc                                                             <  2:421478/150‑1 (MQ=255)
                                                                                         tGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCACGCCCGCCCCCACCAGAAACTGCCGATCCCCCTGAACGGTGGCATAACCCACGACAACCCCCCCCCACAGGTCGCCCAccccgc                                                             >  1:4823592/1‑145 (MQ=255)
                                                                                            ccGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCCGGTGAAACTGCCGAAAGCCGTGTTCGGTGGCATCAACCACGACAACGCCGACCCACGGGGCGCCCACGGCGCCGa                                                          >  1:3872644/1‑150 (MQ=255)
                                                                                             cGCTTGTTCACTCGTGTCCCCACGCCGGGCGCGCCCGCGGCCCCCGTCGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                         <  1:3230092/150‑1 (MQ=255)
                                                                                             cGCTTCTTCAATTCTGTCACCACGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                         <  2:3224917/150‑1 (MQ=255)
                                                                                             cGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGAc                                                         >  2:917612/1‑150 (MQ=255)
                                                                                             cGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCGCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGAc                                                         >  2:2500042/1‑150 (MQ=255)
                                                                                                   ttcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCCGCTCGAGCAGGCCCGCGCCCAGGTGAAACAGCCGATCGCCGTGAACGGTGGCATCCCCCTCGACAACGCCGCCCCACGGGTCGCCCACGGCGCCGACcagctg                                                   >  1:1624690/1‑150 (MQ=255)
                                                                                                    tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCCACGTCGGCCGGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTgg                                                  <  1:504975/150‑1 (MQ=255)
                                                                                                    tcAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCGCCCCACAGGTCGCCCACGGCGCCGACCGGCTgg                                                  >  1:2229526/1‑150 (MQ=255)
                                                                                                        ttCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCACGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCACGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCAGCAGGCGGt                                              >  1:4537611/1‑150 (MQ=255)
                                                                                                        ttCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCCCCCCCACGGGAAACAGCCGATCGCCCGGATCGGTGGCATCAACCACGACAACACCGCCCCACTCGTCGCCCACCGCGCCGACCAGCAGGAGGt                                              >  1:2335604/1‑150 (MQ=255)
                                                                                                        ttCTGCCACCACGCGGGGCGCGCCCGCTCCCCCCGCCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGt                                              <  1:2039939/150‑1 (MQ=255)
                                                                                                          cTGCCACCACGCCGGGCGCGCCGGCCGCCACCGTCGGCCTGCGCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGa                                            <  2:300668/150‑1 (MQ=255)
                                                                                                             tCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATcc                                         >  2:1511349/1‑150 (MQ=255)
                                                                                                              ccccccgCCGGGCGCGCCCGCCGCCACCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCa                                        <  2:3763834/144‑1 (MQ=255)
                                                                                                               aCCACGCGGGGCGCGCCCGCTGCCACCGTCGGCCTGATCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCAc                                       <  1:5626684/150‑1 (MQ=255)
                                                                                                               aCCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCGGCGCGAGCCGGCCCGCGCCCACGTGAAACGGCCGATCGCCGGGATCGGTGGCAGCACCCACGACAACGCCCCCCCACGCGTCGCCCACCGCGCCGCCCAGCAGGCGGGGATCCAc                                       >  1:3045338/1‑150 (MQ=255)
                                                                                                                ccAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCGCGAGCAGGCCCGCGCCCACGTGAAACTGCCGAACGCCGTGATCGGTGGCATCACCCTCGAAAAAGCCGCCCCACTGGTCGCCCACGGCGCCGACCGGCTGGCGGGGCTCCACg                                      >  1:1410842/1‑150 (MQ=255)
                                                                                                                 cccgccggccgCGCCCCCGGCCCCCGGCGGCCTGCGCGCCCCGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                     <  1:2859770/147‑1 (MQ=255)
                                                                                                                 cAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                     >  2:1539912/1‑150 (MQ=255)
                                                                                                                 cAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACgg                                     >  2:181546/1‑150 (MQ=255)
                                                                                                                         gcgcgcCCGCCGCCCCCGGCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCg                             <  1:6180818/150‑1 (MQ=255)
                                                                                                                             ccccGCTGCCCCCGGCGGCCGGCTCGCGCCGGCCCGCGCCCAGGTGAAACTGCCAATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGc                         <  1:3427254/149‑1 (MQ=255)
                                                                                                                                  cTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACa                    <  2:5619323/150‑1 (MQ=255)
                                                                                                                                    gCCACCGCCGGCCGGCGCCAGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgc                  <  1:1270022/150‑1 (MQ=255)
                                                                                                                                     ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg                 >  2:4983190/1‑150 (MQ=255)
                                                                                                                                     ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg                 >  1:1008560/1‑150 (MQ=255)
                                                                                                                                     ccAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGGCGCCCACGGCGCCGACCGGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcg                 >  2:1649975/1‑150 (MQ=255)
                                                                                                                                       aaCGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCAGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAgcgcg               >  1:5937746/1‑150 (MQ=255)
                                                                                                                                          gggggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAg            <  2:5411590/147‑1 (MQ=255)
                                                                                                                                          ggcGGCCTGCGCCCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAg            <  1:4600584/148‑1 (MQ=255)
                                                                                                                                          gcgggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAg            >  1:6097524/4‑150 (MQ=255)
                                                                                                                                          gcgggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAg            >  1:5203613/4‑150 (MQ=255)
                                                                                                                                             ggCCTGCTCGCGCCGCCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa         <  2:1236006/150‑1 (MQ=255)
                                                                                                                                             ggCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa         <  2:3344095/150‑1 (MQ=255)
                                                                                                                                             ggCCTGCGCGCGCCGGCCCGCGCCCAGGTGACACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGaa         <  2:3147116/150‑1 (MQ=255)
                                                                                                                                              gCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAg        <  2:6519832/150‑1 (MQ=255)
                                                                                                                                              gCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAg        >  1:5681130/1‑150 (MQ=255)
                                                                                                                                               ccTGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGt       <  2:5674968/150‑1 (MQ=255)
                                                                                                                                               ccTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGt       >  2:6140095/1‑150 (MQ=255)
                                                                                                                                                cTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCTGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTc      >  2:3611095/1‑150 (MQ=255)
                                                                                                                                                 tGCTCGCGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCa     <  1:492841/150‑1 (MQ=255)
                                                                                                                                                  gCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAc    >  2:4495990/1‑150 (MQ=255)
                                                                                                                                                  gCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAc    >  2:2200218/1‑150 (MQ=255)
                                                                                                                                                   cTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAcc   >  2:609342/1‑150 (MQ=255)
                                                                                                                                                    tCGCGCCGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAccc  <  1:4223542/150‑1 (MQ=255)
                                                                                                                                                    tCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCAccc  >  1:696393/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCCGTCAGGCAATCATCGGCTCCACCTGCCACGCCAGCCTCGAACTGGCCGCCCAGGCTGCCAGCGAAGGCGCCAGCTACGTGGCCTTTGGCCGCTTCTTCAATTCTGTCACCAAGCCGGGCGCGCCCGCTGCCAACGTCGGCCTGCTCGAGCAGGCCCGCGCCCAGGTGAAACTGCCGATCGCCGTGATCGGTGGCATCACCCTCGACAACGCCGCCCCACTGGTCGCCCACGGCGCCGACCTGCTGGCGGTGATCCACGGCTTGTTCGGTGCCGACAGCGCGCAGGAAGTCACCC  >  NC_002947/5451141‑5451438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑