BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	464,324	T→A	6.8%	H259L (CAC→CTC) ‡	davB ←	lysine 2‑monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	464,324	0	T	A	6.8%	1120.4 / 45.9	336	H259L (CAC→CTC) ‡	davB	lysine 2‑monooxygenase
Reads supporting (aligned to +/- strand): ref base T (144/169); new base A (10/13); total (154/182)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

CGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTG  >  NC_002947/464175‑464473

  gcggcgCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTg                                                                                                                                                      >  2:3250668/1‑149 (MQ=255)

  gcggcgCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTg                                                                                                                                                      <  1:3250668/149‑1 (MQ=255)

    ggcgCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtg                                                                                                                                                   <  2:26015/150‑1 (MQ=255)

    ggcgCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtg                                                                                                                                                   >  2:4890214/1‑150 (MQ=255)

     gcgcGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtgg                                                                                                                                                  <  2:5357103/150‑1 (MQ=255)

      cgcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggt                                                                                                                                                 <  1:6146730/150‑1 (MQ=255)

      cgcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggt                                                                                                                                                 <  2:2258606/150‑1 (MQ=255)

       gcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggtg                                                                                                                                                >  2:4810523/1‑150 (MQ=255)

         gggCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTggtggt                                                                                                                                              >  2:469948/1‑150 (MQ=255)

           gCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcg                                                                                                                                            <  1:4575885/150‑1 (MQ=255)

             aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                          <  2:6672174/150‑1 (MQ=255)

             aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                          >  2:4570797/1‑150 (MQ=255)

             aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcggg                                                                                                                                          <  2:355544/150‑3 (MQ=255)

             aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTGGTGGCAGTGGTGCCCCGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                          <  2:5236912/150‑1 (MQ=255)

              aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgggg                                                                                                                                         <  2:6295177/150‑4 (MQ=255)

                  gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgggggcgt                                                                                                                                     <  2:1996614/150‑8 (MQ=255)

                  gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgggggcgt                                                                                                                                     <  2:338944/150‑8 (MQ=255)

                        ccccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtca                                                                                                                               >  1:2416878/1‑150 (MQ=255)

                         cccGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtcat                                                                                                                              >  2:3159607/1‑150 (MQ=255)

                                     tGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGaa                                                                                                                  <  1:3884463/150‑1 (MQ=255)

                                       cgccgcCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGa                                                                                                                <  2:1335923/150‑1 (MQ=255)

                                         ccgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGAtt                                                                                                              >  2:1816172/1‑150 (MQ=255)

                                            ccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTcc                                                                                                           <  2:2709056/150‑1 (MQ=255)

                                            ccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTcc                                                                                                           <  1:4149296/150‑1 (MQ=255)

                                                  aGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCacc                                                                                                     >  1:4328226/1‑148 (MQ=255)

                                                    cGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGa                                                                                                   <  1:4457370/150‑1 (MQ=255)

                                                    cGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGa                                                                                                   <  2:6171623/150‑1 (MQ=255)

                                                     gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAg                                                                                                  >  1:3480057/1‑150 (MQ=255)

                                                     gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAg                                                                                                  >  1:3559430/1‑150 (MQ=255)

                                                      tGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGt                                                                                                 <  1:2565596/150‑1 (MQ=255)

                                                      tGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGt                                                                                                 <  2:5861843/150‑1 (MQ=255)

                                                       gCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                                >  1:2189459/1‑150 (MQ=255)

                                                        cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTg                                                                                               >  1:4826667/1‑150 (MQ=255)

                                                         tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                              <  1:1961938/150‑1 (MQ=255)

                                                         tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                              <  2:1357556/150‑1 (MQ=255)

                                                         tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                              >  2:2906747/1‑150 (MQ=255)

                                                         tCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                              >  1:6811712/1‑150 (MQ=255)

                                                          cAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTggg                                                                                             <  1:5226874/150‑1 (MQ=255)

                                                             gCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGaa                                                                                          >  2:5091582/1‑150 (MQ=255)

                                                               tGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGt                                                                                        >  2:2957088/1‑150 (MQ=255)

                                                                ggTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTc                                                                                       <  1:5548791/150‑1 (MQ=255)

                                                                   gCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCCCCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                    <  1:54091/150‑1 (MQ=255)

                                                                   gCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGa                                                                                    <  2:1252645/150‑1 (MQ=255)

                                                                    cccTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAg                                                                                   >  1:1894373/1‑150 (MQ=255)

                                                                      ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                 >  1:6073961/1‑150 (MQ=255)

                                                                      ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                 <  2:2055720/150‑1 (MQ=255)

                                                                      ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                 >  2:4661990/1‑150 (MQ=255)

                                                                      ctctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGCGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                                 >  2:749002/1‑150 (MQ=255)

                                                                       tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                         <  2:2326281/93‑1 (MQ=255)

                                                                       tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                                         >  1:2326281/1‑93 (MQ=255)

                                                                       tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTcc                                                                                >  1:901123/1‑150 (MQ=255)

                                                                       tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTcc                                                                                >  1:3124278/1‑150 (MQ=255)

                                                                         tGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCa                                                                              >  1:5693988/1‑150 (MQ=255)

                                                                           gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                            >  1:4042336/1‑150 (MQ=255)

                                                                           gCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                            >  2:6006877/1‑150 (MQ=255)

                                                                             cAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccg                                                                          >  1:1395664/1‑150 (MQ=255)

                                                                                    cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                   <  1:2576147/150‑1 (MQ=255)

                                                                                    cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                   <  1:6036330/150‑1 (MQ=255)

                                                                                    cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                   <  2:1082578/150‑1 (MQ=255)

                                                                                    cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                   <  1:5009256/150‑1 (MQ=255)

                                                                                     gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGcc                                                                  >  1:706229/1‑150 (MQ=255)

                                                                                     gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGcc                                                                  >  1:3655985/1‑150 (MQ=255)

                                                                                       aGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGa                                                                >  1:6677583/1‑150 (MQ=255)

                                                                                             ccGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGa                                                          >  1:6536731/1‑150 (MQ=255)

                                                                                              cGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAc                                                         <  2:3913320/150‑1 (MQ=255)

                                                                                                  aCGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGCCCTgg                                                     <  1:3178213/150‑1 (MQ=255)

                                                                                                       gcgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                                >  1:4027120/1‑150 (MQ=255)

                                                                                                        cgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAac                                               <  1:2702825/150‑1 (MQ=255)

                                                                                                         gcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAaca                                              <  1:3026984/150‑1 (MQ=255)

                                                                                                            aGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACtt                                           <  2:609203/150‑1 (MQ=255)

                                                                                                              aTGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcg                                         >  2:5171973/1‑150 (MQ=255)

                                                                                                              aTGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGATGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcg                                         >  2:3734296/1‑150 (MQ=255)

                                                                                                               tGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCACAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgc                                        <  2:6421969/150‑1 (MQ=255)

                                                                                                                 ccTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                      <  2:6845549/150‑1 (MQ=255)

                                                                                                                       ggCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                                <  2:166087/150‑1 (MQ=255)

                                                                                                                         cACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGaa                              <  1:1119559/150‑1 (MQ=255)

                                                                                                                         cACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGaa                              <  1:2979708/150‑1 (MQ=255)

                                                                                                                          aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGGCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCGGGAAg                             >  1:4742374/1‑150 (MQ=255)

                                                                                                                             tGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTc                          <  1:4642702/150‑1 (MQ=255)

                                                                                                                                 ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                      >  2:6546993/1‑150 (MQ=255)

                                                                                                                                 ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                      >  2:5638894/1‑150 (MQ=255)

                                                                                                                                  cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                     <  2:6612343/150‑1 (MQ=255)

                                                                                                                                  cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                     >  2:4212078/1‑150 (MQ=255)

                                                                                                                                  cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                     <  1:6501832/150‑1 (MQ=255)

                                                                                                                                  cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                     >  2:4715112/1‑150 (MQ=255)

                                                                                                                                   aCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTg                    >  2:5697079/1‑150 (MQ=255)

                                                                                                                                     gCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGc                  >  1:2724598/1‑150 (MQ=255)

                                                                                                                                       ccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                <  2:481268/150‑1 (MQ=255)

                                                                                                                                       ccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                >  2:4986095/1‑150 (MQ=255)

                                                                                                                                       ccccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa                <  2:6499047/150‑1 (MQ=255)

                                                                                                                                        cccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaa               >  1:1637059/1‑150 (MQ=255)

                                                                                                                                         ccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaa              >  2:1437975/1‑150 (MQ=255)

                                                                                                                                         ccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaa              <  1:6449122/150‑1 (MQ=255)

                                                                                                                                           gACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaata            <  1:5764352/150‑3 (MQ=255)

                                                                                                                                           gACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAaga            <  2:2416878/150‑1 (MQ=255)

                                                                                                                                           gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                   <  1:1437756/127‑1 (MQ=255)

                                                                                                                                           gACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                   >  2:1437608/1‑127 (MQ=255)

                                                                                                                                               accaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAgcgc        >  1:1494661/1‑150 (MQ=255)

                                                                                                                                                     tGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGTCAAAAGAGCGCGAGGTg  <  1:469635/150‑1 (MQ=255)

                                                                                                                                                     tGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAACGAGCGCGAGGTg  <  2:4031239/150‑1 (MQ=255)

CGGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAGGTG  >  NC_002947/464175‑464473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`